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Literature summary for 1.1.1.21 extracted from
- NAD(P)H-dependent aldose reductase from the xylose-assimilating yeast Candida tenuis (1997), Biochem. J., 326, 683-692.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| bovine serum albumin | 10-15% increased activity | Yamadazyma tenuis | |
| Na2SO4 | - |
Yamadazyma tenuis |  |
| Tween | Tween-20, Tween-80, Tween-100, each 0.1% (w/v) | Yamadazyma tenuis | |
General Stability
| General Stability | Organism |
|---|---|
| 25°C, 0.3 M phosphate or Tris, pH 6.0, more than 2 months | Yamadazyma tenuis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AMP | - |
Yamadazyma tenuis | |
| ATP | - |
Yamadazyma tenuis | |
| dithiothreitol | - |
Yamadazyma tenuis | |
| EDTA | - |
Yamadazyma tenuis | |
| ionic detergents | - |
Yamadazyma tenuis | |
| Mn2+ | - |
Yamadazyma tenuis | |
| NADP+ | - |
Yamadazyma tenuis | |
| pyridoxal 5'-phosphate | - |
Yamadazyma tenuis | |
| Zn2+ | - |
Yamadazyma tenuis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | brief investigation of steady state kinetic | Yamadazyma tenuis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
gel filtration | Yamadazyma tenuis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Yamadazyma tenuis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Yamadazyma tenuis |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| alditol + NAD(P)+ = aldose + NAD(P)H + H+ | reaction mechanism | Yamadazyma tenuis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.5 | - |
- |
Yamadazyma tenuis |
| 28.64 | - |
purified enzyme | Yamadazyma tenuis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alditol + NAD(P)+ | 10% oxidation activity compared to reduction reaction | Yamadazyma tenuis | aldose + NAD(P)H | - |
r | |
| aldose + NAD(P)H | wide specificity | Yamadazyma tenuis | alditol + NAD(P)+ | - |
r | |
| D-xylose + NAD(P)H | - |
Yamadazyma tenuis | xylitol + NAD(P)+ | - |
r | |
| pentose + NADPH | - |
Yamadazyma tenuis | pentitol + NADP+ | - |
r | |
| xylitol + NAD(P)+ | - |
Yamadazyma tenuis | D-xylose + NAD(P)H + H+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 43000, SDS-PAGE | Yamadazyma tenuis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Yamadazyma tenuis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 50 | - |
Yamadazyma tenuis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 35 | after 48 h incubation activity decreases | Yamadazyma tenuis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
reduction of D-xylose, NADH | Yamadazyma tenuis |
| 8.9 | - |
oxidation of xylitol, NAD+ | Yamadazyma tenuis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | below pH 5.0 and above pH 8.0 inactivation at 25°C within 3-6 days | Yamadazyma tenuis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Yamadazyma tenuis | |
| NADP+ | 2.2fold higher activity than with NAD+ | Yamadazyma tenuis | |
| NADPH | 5.3fold higher activity than with NAD(H) | Yamadazyma tenuis | |
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