Literature summary for 1.1.1.209 extracted from

El-Kabbani, O.; Ishikura, S.; Wagner, A.; Schulze-Briese, C.; Hara, A.
Crystallization and preliminary X-ray diffraction analysis of mouse 3(17)alpha-hydroxysteroid dehydrogenase (2005), Acta crystallogr. Sect. F, 61, 688-690.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression of isozyme AKR1C21 in Escherichia coli strain JM109	Mus musculus

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant isozyme AKR1C21, hanging drop vapour diffusion method, 16 mg/ml enzyme in 10 mM Tris-HCl, pH 7.5, and 2 mM 2-mercaptoethanol, mixed with an equal volume of 0.003 ml of reservoir solution containing 0.1 M HEPES, pH 7.5, 10% PEG 6000, 5% 2-methyl-2,4-pentanediol, equilibration against 1 ml reservoir solution, 22°C, 1 week, X-ray diffraction structure determination and analysis at 1.8 A resolution, molecular replacement	Mus musculus

Crystallization (Comment)

Organism

purified recombinant isozyme AKR1C21, hanging drop vapour diffusion method, 16 mg/ml enzyme in 10 mM Tris-HCl, pH 7.5, and 2 mM 2-mercaptoethanol, mixed with an equal volume of 0.003 ml of reservoir solution containing 0.1 M HEPES, pH 7.5, 10% PEG 6000, 5% 2-methyl-2,4-pentanediol, equilibration against 1 ml reservoir solution, 22°C, 1 week, X-ray diffraction structure determination and analysis at 1.8 A resolution, molecular replacement

Mus musculus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
39000	-	x * 39000, recombinant isozyme AKR1C21, SDS-PAGE	Mus musculus

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	-	isozyme AKR1C21	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant isozyme AKR1C21 from Escherichia coli strain JM109 to homogeneity	Mus musculus

Source Tissue

Source Tissue	Comment	Organism	Textmining
kidney	-	Mus musculus	-

Subunits

Subunits	Comment	Organism
?	x * 39000, recombinant isozyme AKR1C21, SDS-PAGE	Mus musculus

Synonyms

Synonyms	Comment	Organism
3(17)alpha-hydroxysteroid dehydrogenase	-	Mus musculus
AKR1C21	-	Mus musculus

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Release 2026.1 (March 2026)