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Literature summary for 1.1.1.209 extracted from
- Identification of two dihydrodiol dehydrogenases associated with 3(17)alpha-hydoxysteroid dehydrogenase activity in mouse kidney (1989), J. Biochem., 106, 633-638.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 17beta-estradiol | 5 mM, 77% inhibition | Mus musculus |  |
| 5alpha-Androstan-3beta-ol-17-one | 1 mM, 80% inhibition | Mus musculus | |
| 5alpha-pregnan-3beta-ol-21-one | 1 mM, 66% inhibition | Mus musculus | |
| 5beta-androstan-3beta-ol-17-one | 1 mM, 91% inhibition | Mus musculus | |
| 5beta-Pregnan-3beta-ol-20-one | 1 mM, 80% inhibition | Mus musculus | |
| Barbital | 100 mM, 11% inhibition | Mus musculus | |
| Dienstrol | 1 mM, 22% inhibition | Mus musculus | |
| Hexestrol | 1 mM, 77% inhibition | Mus musculus | |
| Stilbestrol | 10 mM, 75% inhibition | Mus musculus | |
| testosterone | 1 mM, 48% inhibition | Mus musculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0001 | - |
5beta-androstane-3alpha,17beta-diol | cofactor NADP+ | Mus musculus | |
| 0.0001 | - |
Epitestosterone | cofactor NADP+ | Mus musculus | |
| 0.0003 | - |
5beta-androstane-3alpha,17beta-diol | cofactor NADP+ | Mus musculus | |
| 0.0004 | - |
glycolithocholic acid | cofactor NADP+ | Mus musculus | |
| 0.0005 | - |
5beta-pregnane-3alpha,20alpha-diol | cofactor NADP+ | Mus musculus | |
| 0.0007 | - |
5beta-pregnane-3alpha-ol-20-one | cofactor NADP+ | Mus musculus | |
| 0.004 | - |
NADP+ | - |
Mus musculus | |
| 0.16 | - |
(R)-1-indanol | cofactor NADP+ | Mus musculus | |
| 0.26 | - |
NAD+ | - |
Mus musculus | |
| 0.77 | - |
trans-benzene dihydrodiol | cofactor NADP+ | Mus musculus | |
| 1.99 | - |
cis-benzene dihydrodiol | cofactor NADP+ | Mus musculus | |
| 2.23 | - |
cyclohex-2-ene-1-ol | cofactor NADP+ | Mus musculus | |
| 2.67 | - |
Naphthalene dihydrodiol | cofactor NADP+ | Mus musculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
gel filtration | Mus musculus |
| 39000 | - |
1 * 39000, SDS-PAGE | Mus musculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Q-Sepharose, Matrex Red A, HA-Ultrogel, Sephadex G-100, two enzyme preparations DD1 and DD2 with almost identical properties, products of post-translational or post-mortem modification rather than isoenzymes | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.27 | - |
- |
Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-1-indanol + NADP+ | 37% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene | Mus musculus | 1-indanone + NADPH | - |
? | |
| (S)-1-indanole + NADP+ | 7% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene | Mus musculus | 1-indanone + NADPH | - |
? | |
| 1-acenaphthenol + NADP+ | 10% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene | Mus musculus | 1-acenaphthenone + NADPH | - |
? | |
| 4-nitrobenzaldehyde + NADPH | 58% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene | Mus musculus | 4-nitrobenzyl alcohol + NADP+ | - |
? | |
| 5alpha-androstane-3alpha,17beta-diol + NADP+ | 7% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene | Mus musculus | 5alpha-androstane-17beta-ol-3-one + NADPH | - |
? | |
| 5alpha-pregnan-3alpha,20alpha-diol + NADP+ | 6% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene | Mus musculus | 5alpha-pregnan-20alpha-ol-3-one + NADPH | - |
? | |
| 5alpha-pregnan-3alpha,21-diol-20-one + NADP+ | 7% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene | Mus musculus | 5alpha-pregnan-21alpha-ol-3,20-dione + NADPH | - |
? | |
| 5alpha-pregnane-21-ol-3,20-dione + NADPH | 75% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene | Mus musculus | 5beta-pregnane-3alpha,21-diol-20-one + NADP+ | - |
? | |
| 5alpha-pregnane-3,20-dione + NADPH | 10% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene | Mus musculus | 5alpha-pregnane-3alpha-ol-20-one + NADP+ | - |
? | |
| 5beta-androstan-17beta-ol-3-one + NADPH | 10% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene | Mus musculus | 5beta-androstan-17beta,3alpha-diol + NADP+ | - |
? | |
| 5beta-androstane-3,17-dione + NADPH | 10% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene | Mus musculus | 5beta-androstan-3alpha-ol-17-one + NADP+ | - |
? | |
| 5beta-androstane-3alpha,17beta-diol + NADP+ | 11% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene | Mus musculus | 5beta-androstane-17beta-ol-3-one + NADPH | - |
? | |
| 5beta-androstane-3alpha-ol-17-one + NADP+ | 9% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene | Mus musculus | 5beta-androstane-3,17-dione + NADPH | - |
? | |
| 5beta-pregnan-3alpha,20alpha-diol + NADP+ | 26% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene | Mus musculus | 5beta-pregnan-3-one-20alpha-ol + NADPH | - |
? | |
| 5beta-pregnan-3alpha-ol-20-one + NADP+ | 19% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene | Mus musculus | 5beta-pregnan-3,20-dione + NADPH | - |
? | |
| 5beta-pregnane-3,20-dione + NADPH + H+ | 21% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene | Mus musculus | 5beta-pregnane-3alpha-ol-20-one + NADP+ | - |
? | |
| acenaphthenequinone + NADPH | 44% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene | Mus musculus | acenaphthenehydroquinone + NADP+ | - |
? | |
| androst-4-ene-3,17-dione + NADPH | 10% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene | Mus musculus | androst-4-ene-3alpha-ol-17-one + NADP+ | - |
? | |
| camphorquinone + NADPH + H+ | 52% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene | Mus musculus | camphorhydroquinone + NADP+ | - |
? | |
| cis-1,2-dihydroxy-3,5-cyclohexadiene + NADP+ | 60% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene | Mus musculus | catechol + NADPH | - |
? | |
| cyclohex-2-ene-1-ol + NADP+ | 9% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene | Mus musculus | cyclohex-2-en-1-one + NADPH | - |
? | |
| cyclohexanone + NADPH | 16% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene | Mus musculus | cyclohexanol + NADP+ | - |
? | |
| epitestosterone + NADP+ | 11% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene | Mus musculus | testosterone + NADPH + H+ | - |
? | |
| glycolithocholic acid + NADP+ | 56% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene | Mus musculus | N-[(5beta)-3,24-dioxocholan-24-yl]glycine + NADPH | - |
? | |
| naphthalene dihydrodiol + NADP+ | 49% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene | Mus musculus | naphthacatechol + NADPH | - |
? | |
| pyridine-4-ol + NADP+ | 75% of activity with trans-1,2-dihydroxy-3,5-cyclohexadiene | Mus musculus | 3H-pyridine-4-one + NADPH | - |
? | |
| tetrahydrodeoxycorticosterone + NAD+ | - |
Mus musculus | ? | - |
r | |
| trans-1,2-dihydroxy-3,5-cyclohexadiene + NADP+ | - |
Mus musculus | catechol + NADPH | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 39000, SDS-PAGE | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 6.8 | 5beta-pregnane-3,20-dione as substrate, cofactor NADPH | Mus musculus |
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