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Literature summary for 1.1.1.202 extracted from
- High-level expression of the 1,3-propanediol oxidoreductase from Klebsiella pneumoniae in Escherichia coli (2005), Mol. Biotechnol., 31, 211-219.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene dhaT, cloning from genomic DNA, DNA sequence determination, inducible high-level expression of the mostly soluble enzyme in Escherichia coli strain BL21(DE3) | Klebsiella pneumoniae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.21 | - |
NAD+ | pH 9.5, 30°C, recombinant enzyme | Klebsiella pneumoniae |  |
| 8.5 | - |
Propane-1,3-diol | pH 9.5, 30°C, recombinant enzyme | Klebsiella pneumoniae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
x * 42000, recombinant enzyme, SDS-PAGE | Klebsiella pneumoniae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-hydroxypropionaldehyde + NADH + H+ | Klebsiella pneumoniae | the enzyme is important in vivo for convertion of glycerol into propane-1,3-diol as second step after dehydration of glycerol by coenzyme B12-dependent glycerol dehydratase | propane-1,3-diol + NAD+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Klebsiella pneumoniae | - |
gene dhaT | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant soluble enzyme from Escherichia coli strain BL21(DE3) | Klebsiella pneumoniae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-hydroxypropionaldehyde + NADH + H+ | the enzyme is important in vivo for convertion of glycerol into propane-1,3-diol as second step after dehydration of glycerol by coenzyme B12-dependent glycerol dehydratase | Klebsiella pneumoniae | propane-1,3-diol + NAD+ | - |
r | |
| 3-hydroxypropionaldehyde + NADH + H+ | - |
Klebsiella pneumoniae | propane-1,3-diol + NAD+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 42000, recombinant enzyme, SDS-PAGE | Klebsiella pneumoniae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 1,3-propanediol oxidoreductase | - |
Klebsiella pneumoniae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
recombinant enzyme | Klebsiella pneumoniae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
recombinant enzyme | Klebsiella pneumoniae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Klebsiella pneumoniae | |
| NADH | - |
Klebsiella pneumoniae | |
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