Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.198 extracted from

  • Tian, N.; Tang, Y.; Xiong, S.; Tian, D.; Chen, Y.; Wu, D.; Liu, Z.; Liu, S.
    Molecular cloning and functional identification of a novel borneol dehydrogenase from Artemisia annua L. (2015), Ind. Crops Prod., 77, 190-195.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
gene bdh, DNA and amino acid sequence determination and analysis, library construction of genes involved in camphor biosynthesis from different cultivars, sequence comparisons, phylogenetic tree of plant alcohol dehydrogenase, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), semi-quantitative RT-PCR enzyme expression analysis Artemisia annua

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics Artemisia annua
0.0483
-
(+)-borneol pH 9.0, 33°C, recombinant enzyme Artemisia annua

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31040
-
-
Artemisia annua

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(+)-borneol + NAD+ Artemisia annua
-
(+)-camphor + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Artemisia annua A0A191ZDL6
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and desalting gel filtration Artemisia annua

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-borneol + NAD+
-
Artemisia annua (+)-camphor + NADH + H+
-
r
(+)-borneol + NADP+
-
Artemisia annua (+)-camphor + NADPH + H+
-
r
additional information substrate specificity, overview. No activity with other monoterpene alcohol, several kinds of monoterpene alcohols including (-)-artemisia alcohol, cis-carveol, trans-carveol, and trans-pinocarveol. The enzyme is specific for (+)-borneol Artemisia annua ?
-
?

Subunits

Subunits Comment Organism
? x * 31040, sequence calculation Artemisia annua

Synonyms

Synonyms Comment Organism
AaBDH
-
Artemisia annua

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
33
-
recombinant enzyme Artemisia annua

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0058
-
(+)-borneol pH 9.0, 33°C, recombinant enzyme Artemisia annua

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
recombinant enzyme Artemisia annua

Cofactor

Cofactor Comment Organism Structure
additional information enzyme AaBDH is active in the presence of cofactor NADP+ or NAD+, NAD+ is the preferred cofactor Artemisia annua
NAD+
-
Artemisia annua
NADP+ low activity Artemisia annua

pI Value

Organism Comment pI Value Maximum pI Value
Artemisia annua sequence calculation
-
6.16