Cloned (Comment) | Organism |
---|---|
gene bdh, DNA and amino acid sequence determination and analysis, library construction of genes involved in camphor biosynthesis from different cultivars, sequence comparisons, phylogenetic tree of plant alcohol dehydrogenase, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), semi-quantitative RT-PCR enzyme expression analysis | Artemisia annua |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Artemisia annua | |
0.0483 | - |
(+)-borneol | pH 9.0, 33°C, recombinant enzyme | Artemisia annua |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31040 | - |
- |
Artemisia annua |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-borneol + NAD+ | Artemisia annua | - |
(+)-camphor + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Artemisia annua | A0A191ZDL6 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and desalting gel filtration | Artemisia annua |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-borneol + NAD+ | - |
Artemisia annua | (+)-camphor + NADH + H+ | - |
r | |
(+)-borneol + NADP+ | - |
Artemisia annua | (+)-camphor + NADPH + H+ | - |
r | |
additional information | substrate specificity, overview. No activity with other monoterpene alcohol, several kinds of monoterpene alcohols including (-)-artemisia alcohol, cis-carveol, trans-carveol, and trans-pinocarveol. The enzyme is specific for (+)-borneol | Artemisia annua | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 31040, sequence calculation | Artemisia annua |
Synonyms | Comment | Organism |
---|---|---|
AaBDH | - |
Artemisia annua |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
33 | - |
recombinant enzyme | Artemisia annua |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0058 | - |
(+)-borneol | pH 9.0, 33°C, recombinant enzyme | Artemisia annua |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
recombinant enzyme | Artemisia annua |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme AaBDH is active in the presence of cofactor NADP+ or NAD+, NAD+ is the preferred cofactor | Artemisia annua | |
NAD+ | - |
Artemisia annua | |
NADP+ | low activity | Artemisia annua |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Artemisia annua | sequence calculation | - |
6.16 |