Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Streptomyces sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.28 | - |
coniferyl alcohol | recombinant enzyme, at pH 8.5 and 40°C | Streptomyces sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | dependent on | Streptomyces sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
4 * 39000, SDS-PAGE | Streptomyces sp. |
150000 | - |
gel filtration | Streptomyces sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. | M5AJW4 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Resource PHE column chromatography, and Mono Q Superdex 200 column chromatography | Streptomyces sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.27 | - |
cell-free extract, at pH 8.5 and 40°C | Streptomyces sp. |
10.7 | - |
after 40fold purification, at pH 8.5 and 40°C | Streptomyces sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
coniferyl alcohol + NADP+ | - |
Streptomyces sp. | coniferyl aldehyde + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 39000, SDS-PAGE | Streptomyces sp. |
homotetramer | 4 * 37845, calculated from amino acid sequence | Streptomyces sp. |
Synonyms | Comment | Organism |
---|---|---|
CADH | - |
Streptomyces sp. |
coniferyl alcohol dehydrogenase | - |
Streptomyces sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Streptomyces sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
25.8 | - |
coniferyl alcohol | recombinant enzyme, at pH 8.5 and 40°C | Streptomyces sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Streptomyces sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Streptomyces sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
93.3 | - |
coniferyl alcohol | recombinant enzyme, at pH 8.5 and 40°C | Streptomyces sp. |