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Literature summary for 1.1.1.192 extracted from

  • Liu, X.; Dong, Y.; Zhang, J.; Zhang, A.; Wang, L.; Feng, L.
    Two novel metal-independent long-chain alkyl alcohol dehydrogenases from Geobacillus thermodenitrificans NG80-2 (2009), Microbiology, 155, 2078-2085.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
EDTA 1 mM, 105% of initial activity Geobacillus thermodenitrificans

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Geobacillus thermodenitrificans

Inhibitors

Inhibitors Comment Organism Structure
Al3+ 1 mM, 14.7% residual activity; 1 mM, 18.9% residual activity Geobacillus thermodenitrificans
Ca2+ 1 mM, no residual activity Geobacillus thermodenitrificans
Co2+ 1 mM, 51.9% residual activity; 1 mM, 68.7% residual activity Geobacillus thermodenitrificans
Cu2+ 1 mM, 14.0% residual activity; 1 mM, 44.2% residual activity Geobacillus thermodenitrificans
K+ 1 mM, 70.3% residual activity; 1 mM, 82.3% residual activity Geobacillus thermodenitrificans
Mg2+ 1 mM, 0.3% residual activity; 1 mM, 74.3% residual activity Geobacillus thermodenitrificans
Mn2+ 1 mM, 14.3% residual activity; 1 mM, 23.4% residual activity Geobacillus thermodenitrificans
Ni2+ 1 mM, 18.2% residual activity; 1 mM, 72.7% residual activity Geobacillus thermodenitrificans
sodium dodecylsulfate 1 mM, 3% residual activity; 1 mM, no residual activity Geobacillus thermodenitrificans
Zn2+ 1 mM, 14.1% residual activity; 1 mM, 30.5% residual activity Geobacillus thermodenitrificans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.84
-
NADPH cosubstrate octanal, pH 8.0, 60°C Geobacillus thermodenitrificans
1.15
-
octanal cosubstrate NADPH, pH 8.0, 60°C Geobacillus thermodenitrificans
1.44
-
NAD+ cosubstrate 1-octanol, pH 8.0, 60°C Geobacillus thermodenitrificans
2.4
-
NADP+ cosubstrate 1-octanol, pH 8.0, 60°C Geobacillus thermodenitrificans
3.88
-
1-Octanol cosubstrate NADP+, pH 8.0, 60°C Geobacillus thermodenitrificans

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ 1 mM, 136% of initial activity Geobacillus thermodenitrificans
Fe2+ 1 mM, 168% of initial activity Geobacillus thermodenitrificans
additional information enzyme contains neither Fe nor other metals, and Fe is not required for the activity, although sequence analysis reveals that ADH1 belongs to the Fe-containing/activated long-chain alcohol dehydrogenases Geobacillus thermodenitrificans
Na+ 1 mM, 110% of initial activity Geobacillus thermodenitrificans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41664
-
8 * 45000, SDS-PAGE, 8 * 41664, calculated Geobacillus thermodenitrificans
45000
-
8 * 45000, SDS-PAGE, 8 * 41664, calculated Geobacillus thermodenitrificans
90000
-
PAGE Geobacillus thermodenitrificans
380000
-
PAGE Geobacillus thermodenitrificans

Organism

Organism UniProt Comment Textmining
Geobacillus thermodenitrificans A4IP64
-
-
Geobacillus thermodenitrificans A4ISB9
-
-
Geobacillus thermodenitrificans NG80-2 A4IP64
-
-
Geobacillus thermodenitrificans NG80-2 A4ISB9
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,3-propanediol + 2 NAD+ + H2O about 45% of the activity with ethanol Geobacillus thermodenitrificans ? + 2 NADH + 2 H+
-
r
1,3-propanediol + 2 NAD+ + H2O about 5% of the activity with 1-octanol Geobacillus thermodenitrificans ? + 2 NADH + 2 H+
-
r
1,3-propanediol + 2 NAD+ + H2O about 45% of the activity with ethanol Geobacillus thermodenitrificans NG80-2 ? + 2 NADH + 2 H+
-
r
1,3-propanediol + 2 NAD+ + H2O about 5% of the activity with 1-octanol Geobacillus thermodenitrificans NG80-2 ? + 2 NADH + 2 H+
-
r
1-decanol + 2 NAD+ + H2O about 20% of the activity with ethanol Geobacillus thermodenitrificans decanoic acid + 2 NADH + 2 H+
-
r
1-decanol + 2 NAD+ + H2O about 50% of the activity with 1-octanol Geobacillus thermodenitrificans decanoic acid + 2 NADH + 2 H+
-
r
1-decanol + 2 NAD+ + H2O about 20% of the activity with ethanol Geobacillus thermodenitrificans NG80-2 decanoic acid + 2 NADH + 2 H+
-
r
1-decanol + 2 NAD+ + H2O about 50% of the activity with 1-octanol Geobacillus thermodenitrificans NG80-2 decanoic acid + 2 NADH + 2 H+
-
r
1-dodecanol + 2 NAD+ + H2O about 17% of the activity with ethanol Geobacillus thermodenitrificans dodecanoic acid + 2 NADH + 2 H+
-
r
1-dodecanol + 2 NAD+ + H2O about 30% of the activity with 1-octanol Geobacillus thermodenitrificans dodecanoic acid + 2 NADH + 2 H+
-
r
1-dodecanol + 2 NAD+ + H2O about 17% of the activity with ethanol Geobacillus thermodenitrificans NG80-2 dodecanoic acid + 2 NADH + 2 H+
-
r
1-dodecanol + 2 NAD+ + H2O about 30% of the activity with 1-octanol Geobacillus thermodenitrificans NG80-2 dodecanoic acid + 2 NADH + 2 H+
-
r
1-eicosanol + 2 NAD+ + H2O about 25% of the activity with 1-octanol Geobacillus thermodenitrificans eicosanoic acid + 2 NADH + 2 H+
-
r
1-eicosanol + 2 NAD+ + H2O about 3% of the activity with ethanol Geobacillus thermodenitrificans eicosanoic acid + 2 NADH + 2 H+
-
r
1-eicosanol + 2 NAD+ + H2O about 3% of the activity with ethanol Geobacillus thermodenitrificans NG80-2 eicosanoic acid + 2 NADH + 2 H+
-
r
1-eicosanol + 2 NAD+ + H2O about 25% of the activity with 1-octanol Geobacillus thermodenitrificans NG80-2 eicosanoic acid + 2 NADH + 2 H+
-
r
1-hexadecanol + 2 NAD+ + H2O about 10% of the activity with ethanol Geobacillus thermodenitrificans hexadecanoic acid + 2 NADH + 2 H+
-
r
1-hexadecanol + 2 NAD+ + H2O about 25% of the activity with 1-octanol Geobacillus thermodenitrificans hexadecanoic acid + 2 NADH + 2 H+
-
r
1-octacosanol + 2 NAD+ + H2O about 40% of the activity with ethanol Geobacillus thermodenitrificans octacosanoic acid + 2 NADH + 2 H+
-
r
1-octacosanol + 2 NAD+ + H2O about 5% of the activity with 1-octanol Geobacillus thermodenitrificans octacosanoic acid + 2 NADH + 2 H+
-
r
1-octadecanol + 2 NAD+ + H2O about 15% of the activity with 1-octanol Geobacillus thermodenitrificans octadecanoic acid + 2 NADH + 2 H+
-
r
1-octadecanol + 2 NAD+ + H2O about 5% of the activity with ethanol Geobacillus thermodenitrificans octadecanoic acid + 2 NADH + 2 H+
-
r
1-octanol + 2 NAD+ + H2O
-
Geobacillus thermodenitrificans octanoic acid + 2 NADH + 2 H+
-
r
1-octanol + 2 NADP+ + H2O
-
Geobacillus thermodenitrificans octanoic acid + 2 NADPH + 2 H+
-
r
1-tetracosanol + 2 NAD+ + H2O about 10% of the activity with 1-octanol Geobacillus thermodenitrificans tetracosanoic acid + 2 NADH + 2 H+
-
r
1-tetracosanol + 2 NAD+ + H2O about 30% of the activity with ethanol Geobacillus thermodenitrificans tetracosanoic acid + 2 NADH + 2 H+
-
r
1-tetradecanol + 2 NAD+ + H2O about 15% of the activity with ethanol Geobacillus thermodenitrificans tetradecanoic acid + 2 NADH + 2 H+
-
r
1-tetradecanol + 2 NAD+ + H2O about 30% of the activity with 1-octanol Geobacillus thermodenitrificans tetradecanoic acid + 2 NADH + 2 H+
-
r
decanal + NAD+ + H+ about 10% of the activity with 1-octanol Geobacillus thermodenitrificans decanoic acid + NADH
-
r
dodecanal + NAD+ + H+ about 10% of the activity with 1-octanol Geobacillus thermodenitrificans dodecanoic acid + NADH
-
r
glycerol + 2 NAD+ + H2O about 45% of the activity with ethanol Geobacillus thermodenitrificans ? + 2 NADH + 2 H+
-
r
isopropanol + 2 NAD+ + H2O about 15% of the activity with 1-octanol Geobacillus thermodenitrificans ? + 2 NADH + 2 H+
-
r
additional information substrates are a broad range of alkyl alcohols from ethanol to 1-triacontanol Geobacillus thermodenitrificans ?
-
?
additional information substrates are a broad range of alkyl alcohols from ethanol to 1-triacontanol Geobacillus thermodenitrificans NG80-2 ?
-
?
octanal + NAD+ + H2O
-
Geobacillus thermodenitrificans octanoic acid + NADH + H+
-
r
octanal + NAD+ + H2O
-
Geobacillus thermodenitrificans NG80-2 octanoic acid + NADH + H+
-
r

Subunits

Subunits Comment Organism
octamer 8 * 45000, SDS-PAGE, 8 * 41664, calculated Geobacillus thermodenitrificans

Synonyms

Synonyms Comment Organism
ADH1
-
Geobacillus thermodenitrificans
ADH2
-
Geobacillus thermodenitrificans
long-chain alkyl alcohol dehydrogenase
-
Geobacillus thermodenitrificans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Geobacillus thermodenitrificans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
14 h, more than 50% of initial activity Geobacillus thermodenitrificans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
38.5
-
NAD+ cosubstrate 1-octanol, pH 8.0, 60°C Geobacillus thermodenitrificans
283.1
-
NADP+ cosubstrate 1-octanol, pH 8.0, 60°C Geobacillus thermodenitrificans
4808
-
NADPH cosubstrate octanal, pH 8.0, 60°C Geobacillus thermodenitrificans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Geobacillus thermodenitrificans

pH Range

pH Minimum pH Maximum Comment Organism
6 9
-
Geobacillus thermodenitrificans

Cofactor

Cofactor Comment Organism Structure
NAD+ NAD+ is prefered over NADP+ Geobacillus thermodenitrificans
NAD+ NADP+ is prefered over NAD+ Geobacillus thermodenitrificans
NADH
-
Geobacillus thermodenitrificans
NADP+ NAD+ is prefered over NADP+ Geobacillus thermodenitrificans
NADP+ NADP+ is prefered over NAD+ Geobacillus thermodenitrificans
NADPH
-
Geobacillus thermodenitrificans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
26.7
-
NAD+ cosubstrate 1-octanol, pH 8.0, 60°C Geobacillus thermodenitrificans
67.2
-
1-Octanol cosubstrate NADP+, pH 8.0, 60°C Geobacillus thermodenitrificans
117.9
-
NADP+ cosubstrate 1-octanol, pH 8.0, 60°C Geobacillus thermodenitrificans
450.8
-
octanal cosubstrate NADPH, pH 8.0, 60°C Geobacillus thermodenitrificans
5724
-
NADPH cosubstrate octanal, pH 8.0, 60°C Geobacillus thermodenitrificans