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Literature summary for 1.1.1.159 extracted from

  • Lou, D.; Wang, B.; Tan, J.; Zhu, L.
    Carboxyl-terminal and Arg38 are essential for activity of the 7alpha-hydroxysteroid dehydrogenase from Clostridium absonum (2014), Protein Pept. Lett., 21, 894-900.
    View publication on PubMed
Search for more literature for 1.1.1.159

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of wild-type and truncation mutant enzymes in Escherichia coli Clostridium sardiniense

Protein Variants

Protein Variants Comment Organism
additional information generation of a C-terminaly truncation enzyme mutant. When 2 and 6 amino acids of C-terminal are removed, the catalytic efficiency of Ca7alpha-HSDH remains 19.1% and 2.5%, respectively. No activity remains after deletion of 8, 14 and 17 amino acids Clostridium sardiniense
R38D site-directed mutagenesis, inactive mutant Clostridium sardiniense

Metals/Ions

Metals/Ions Comment Organism Structure
K+ activates by 105.7% at 500 mM Clostridium sardiniense
Mg2+ activates by 61.4% at 50 mM Clostridium sardiniense
Na+ activates by 64.7% at 200 mM Clostridium sardiniense

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cholate + NAD+ Clostridium sardiniense
-
 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholanate + NADH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Clostridium sardiniense G9FRD7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cholate + NAD+
-
 Clostridium sardiniense 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholanate + NADH + H+
-
 r

Synonyms

Synonyms Comment Organism
7alpha-HSDH
-
 Clostridium sardiniense
Ca7alpha-HSDH
-
 Clostridium sardiniense

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 37 purified recombinant enzyme, no change in activity after incubation at 4°C or 25°C for 108 h, but dramatic decrease in activity at 37°C Clostridium sardiniense

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Clostridium sardiniense

General Information

General Information Comment Organism
evolution 7alpha-hydroxysteroid dehydrogenase is a member of the short-chain dehydrogenase/reductase superfamily Clostridium sardiniense
additional information the C-terminus and residue Arg38 are essential for activity of the 7alpha-hydroxysteroid dehydrogenase from Clostridium absonum Clostridium sardiniense
physiological function the enzyme catalyzes the dehydrogenation of C7 hydroxyl group of the steroid skeleton of bile acids Clostridium sardiniense