Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Bacteroides fragilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
7-keto-lithocholic acid + NADH | Bacteroides fragilis | in the gastrointestinal tract of host organisms | cheno-deoxycholic acid + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacteroides fragilis | - |
strain ATCC 25285 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli by heat treatment at 55-60°C, polyethyleneimine treatment, ammonium sulfate fractionation, and gel filtration, followed by lyophilization | Bacteroides fragilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.32 | - |
purified recombinant enzyme, substrate methyl benzoylformate | Bacteroides fragilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7-keto-lithocholic acid + NADH | - |
Bacteroides fragilis | cheno-deoxycholic acid + NAD+ | - |
? | |
7-keto-lithocholic acid + NADH | in the gastrointestinal tract of host organisms | Bacteroides fragilis | cheno-deoxycholic acid + NAD+ | - |
? | |
ethyl (R)-2-hydroxy-2-(3,5-difluorophenyl)acetate + NAD+ | - |
Bacteroides fragilis | ethyl 2-oxo-2-(3,5-difluorophenyl)acetate + NADH | - |
? | |
ethyl (R)-2-hydroxy-3,3-dimethylbutyrate + NAD+ | - |
Bacteroides fragilis | ethyl 2-oxo-3,3-dimethylbutyrate + NADH | - |
? | |
ethyl (R)-2-hydroxy-3-methylbutyrate + NAD+ | - |
Bacteroides fragilis | ethyl 2-oxo-3-methylbutyrate + NADH | - |
? | |
ethyl 2-hydroxy-2-(3,4-dichlorophenyl)acetate + NAD+ | - |
Bacteroides fragilis | ethyl 2-oxo-2-(3,4-dichlorophenyl)acetate + NADH | - |
? | |
ethyl 2-hydroxy-2-(4-bromophenyl)acetate + NAD+ | - |
Bacteroides fragilis | ethyl 2-oxo-2-(4-bromophenyl)acetate + NADH | - |
? | |
ethyl 2-hydroxy-2-(4-chlorophenyl)acetate + NAD+ | - |
Bacteroides fragilis | ethyl 2-oxo-2-(4-chlorophenyl)acetate + NADH | - |
? | |
ethyl 2-hydroxy-2-(4-cyanophenyl)acetate + NAD+ | high activity | Bacteroides fragilis | ethyl 2-oxo-2-(4-cyanophenyl)acetate + NADH | - |
? | |
ethyl 2-hydroxy-2-(4-fluorophenyl)acetate + NAD+ | - |
Bacteroides fragilis | ethyl 2-oxo-2-(4-fluorophenyl)acetate + NADH | - |
? | |
ethyl 2-hydroxy-2-(4-methylphenyl)acetate + NAD+ | - |
Bacteroides fragilis | ethyl 2-oxo-2-(4-methylphenyl)acetate + NADH | - |
? | |
ethyl 2-hydroxy-2-cyclohexylacetate + NAD+ | best substrate | Bacteroides fragilis | ethyl 2-oxo-2-cyclohexylacetate + NADH | - |
? | |
ethyl 2-hydroxy-2-phenylacetate + NAD+ | - |
Bacteroides fragilis | ethyl 2-oxo-2-phenylacetate + NADH | - |
? | |
methyl 2-hydroxy-2-phenylacetate + NAD+ | - |
Bacteroides fragilis | methyl 2-oxo-2-phenylacetate + NADH | - |
? | |
methyl benzoylformate + NADH | - |
Bacteroides fragilis | ? | - |
? | |
additional information | substrate specificity and stereoselectivity towards various ketones, product determination, overview | Bacteroides fragilis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
7-HSDH | - |
Bacteroides fragilis |
7alpha-hydroxysteroid dehydrogenase | - |
Bacteroides fragilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Bacteroides fragilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the enzyme is thermostable | Bacteroides fragilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Bacteroides fragilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Bacteroides fragilis |