KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
cholic acid | - |
Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
SDS-PAGE | Pseudomonas sp. |
108000 | - |
gel filtration | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chenodeoxycholic acid + NAD+ | - |
Pseudomonas sp. | 3alpha-hydroxy-7-oxo-5beta-cholan-24-oic acid + NADH | - |
? | |
cholic acid + NAD+ | - |
Pseudomonas sp. | 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholan-24-oic acid + NADH | - |
? | |
additional information | catalyzes the oxido-reduction of 7alpha-hydroxy bile acids including glycine and taurine conjugates | Pseudomonas sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 25000, subunit mass by SDS-PAGE, native mass by gel filtration | Pseudomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
7alpha-HSD | - |
Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | highly NAD+ dependent | Pseudomonas sp. | |
thio-NAD+ | utilized with the same activity as NAD+ | Pseudomonas sp. |