Any feedback?
Literature summary for 1.1.1.159 extracted from
- Cloning and characterization of the NAD-dependent 7alpha-Hydroxysteroid dehydrogenase from Bacteroides fragilis (2003), Curr. Microbiol., 47, 475-484.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Bacteroides fragilis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| HgCl2 | complete inhibition at 7.5 mM | Bacteroides fragilis |  |
| p-hydroxymercuribenzoate | complete inhibition at 0.0075 mM | Bacteroides fragilis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.024 | - |
chenodeoxycholic acid | pH 8.5, 37°C | Bacteroides fragilis | |
| 0.177 | - |
cholic acid | pH 8.5, 37°C | Bacteroides fragilis | |
| 0.212 | - |
NAD+ | with cholic acid as substrate, pH 8.5, 37°C | Bacteroides fragilis | |
| 0.709 | - |
NAD+ | with chenodeoxycholic acid as substrate, pH 8.5, 37°C | Bacteroides fragilis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27400 | - |
SDS-PAGE | Bacteroides fragilis |
| 110000 | - |
gel filtration | Bacteroides fragilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacteroides fragilis | Q9S3U5 | strain ATCC 25285 | - |
| Escherichia coli | - |
- |
- |
| Paeniclostridium sordellii | - |
strain ATCC 9714 | - |
| [Clostridium] scindens | - |
formerly Eubacterium sp. strain VP1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacteroides fragilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 351 | - |
purified enzyme, 37°C | Bacteroides fragilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chenodeoxycholic acid + NAD+ | - |
Escherichia coli | 3alpha-hydroxy-7-oxo-5beta-cholan-24-oic acid + NADH | - |
r | |
| chenodeoxycholic acid + NAD+ | - |
Paeniclostridium sordellii | 3alpha-hydroxy-7-oxo-5beta-cholan-24-oic acid + NADH | - |
r | |
| chenodeoxycholic acid + NAD+ | - |
[Clostridium] scindens | 3alpha-hydroxy-7-oxo-5beta-cholan-24-oic acid + NADH | - |
r | |
| chenodeoxycholic acid + NAD+ | - |
Bacteroides fragilis | 3alpha-hydroxy-7-oxo-5beta-cholan-24-oic acid + NADH | - |
r | |
| cholic acid + NAD+ | - |
Escherichia coli | 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholan-24-oic acid + NADH | - |
r | |
| cholic acid + NAD+ | - |
Paeniclostridium sordellii | 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholan-24-oic acid + NADH | - |
r | |
| cholic acid + NAD+ | - |
[Clostridium] scindens | 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholan-24-oic acid + NADH | - |
r | |
| cholic acid + NAD+ | - |
Bacteroides fragilis | 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholan-24-oic acid + NADH | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 27400, subunit mass by SDS-PAGE, native mass by gel filtration | Bacteroides fragilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
complete loss of activity within 30 min | Escherichia coli |
| 65 | - |
complete loss of activity within 30 min | Paeniclostridium sordellii |
| 65 | - |
complete loss of activity within 30 min | [Clostridium] scindens |
| 65 | - |
95% of initial activity after 1 h | Bacteroides fragilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
NADH oxidation | Bacteroides fragilis |
| 8.5 | - |
NAD reduction | Bacteroides fragilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 10.5 | NAD reduction | Bacteroides fragilis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Escherichia coli | |
| NAD+ | - |
Paeniclostridium sordellii | |
| NAD+ | - |
[Clostridium] scindens | |
| NAD+ | - |
Bacteroides fragilis | |
| NADH | - |
Bacteroides fragilis | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
