Cloned (Comment) | Organism |
---|---|
expresed as a His-tagged fusion protein in Escherichia coli | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
crystal structure of AllD in its apo form is determined at 2.13 A resolution, as well as a binary complex at 1.64 A resolution with the NADH cofactor, and a ternary complex at 1.77 A resolution with NADH and glyoxylate, a product yielded from the spontaneous degradation of oxalurate | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D141A | Km ((S)-ureidoglycolate) increased, kcat (S-ureidoglycolate) or (NAD+) decreased compared to wild-type, Km (NAD+) similar to wo wild-type | Escherichia coli |
D141E | Km ((S)-ureidoglycolate) increased, kcat ((S)-ureidoglycolate) or (NAD+) decreased compared to wild-type, Km (NAD+) decreased compared to wild-type | Escherichia coli |
D141N | Km ((S)-ureidoglycolate) or (NAD+) increased, kcat ((S)-ureidoglycolate) or (NAD+) decreased compared to wild-type | Escherichia coli |
H116A | mutant(S)-ureidoglycolate shows no activity | Escherichia coli |
H44A | Km ((S)-ureidoglycolate) increased, kcat (S-ureidoglycolate) or (NAD+) decreased compared to wild-type, Km (NAD+) equal to wild-type | Escherichia coli |
M251A | Km ((S)-ureidoglycolate) or (NAD+) increased, kcat ((S)-ureidoglycolate) or (NAD+) decreased compared to wild-type | Escherichia coli |
R259A | Km ((S)-ureidoglycolate) or (NAD+) increased, kcat ((S)-ureidoglycolate) or (NAD+) decreased compared to wild-type | Escherichia coli |
R48A | Km ((S)-ureidoglycolate) or (NAD+) increased, kcat ((S)-ureidoglycolate) or (NAD+) decreased compared to wild-type | Escherichia coli |
S140A | Km ((S)-ureidoglycolate) or (NAD+) increased, kcat ((S)-ureidoglycolate) or (NAD+) decreased compared to wild-type | Escherichia coli |
S43A | Km ((S)-ureidoglycolate) or (NAD+) increased, kcat ((S)-ureidoglycolate) or (NAD+) decreased compared to wild-type | Escherichia coli |
Y52F | Km ((S)-ureidoglycolate) or (NAD+) increased, kcat ((S)-ureidoglycolate) or (NAD+) decreased compared to wild-type | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.37 | - |
NAD+ | mutant D141E, pH 8.1, 30°C | Escherichia coli | |
0.52 | - |
NAD+ | mutant D141A, pH 8.1, 30°C | Escherichia coli | |
0.56 | - |
NAD+ | wild-type, pH 8.1, 30°C | Escherichia coli | |
0.65 | - |
NAD+ | mutant H44A, pH 8.1, 30°C | Escherichia coli | |
0.81 | - |
NAD+ | mutant D141N, pH 8.1, 30°C | Escherichia coli | |
0.93 | - |
NAD+ | mutant M251A, pH 8.1, 30°C | Escherichia coli | |
1.06 | - |
(S)-ureidoglycolate | wild-type, pH 8.1, 30°C | Escherichia coli | |
1.26 | - |
NAD+ | mutant Y52F, pH 8.1, 30°C | Escherichia coli | |
1.39 | - |
NAD+ | mutant S140A, pH 8.1, 30°C | Escherichia coli | |
1.49 | - |
NAD+ | mutant R259A, pH 8.1, 30°C | Escherichia coli | |
2.28 | - |
NAD+ | mutant S43A, pH 8.1, 30°C | Escherichia coli | |
5.27 | - |
(S)-ureidoglycolate | mutant D141A, pH 8.1, 30°C | Escherichia coli | |
5.87 | - |
(S)-ureidoglycolate | mutant D141N, pH 8.1, 30°C | Escherichia coli | |
10.75 | - |
(S)-ureidoglycolate | mutant S43A, pH 8.1, 30°C | Escherichia coli | |
11.47 | - |
(S)-ureidoglycolate | mutant R259A, pH 8.1, 30°C | Escherichia coli | |
12.38 | - |
(S)-ureidoglycolate | mutant S140A, pH 8.1, 30°C | Escherichia coli | |
13.15 | - |
(S)-ureidoglycolate | mutant M251A, pH 8.1, 30°C | Escherichia coli | |
14.13 | - |
(S)-ureidoglycolate | mutant D141E, pH 8.1, 30°C | Escherichia coli | |
16.82 | - |
(S)-ureidoglycolate | mutant Y52F, pH 8.1, 30°C | Escherichia coli | |
16.94 | - |
(S)-ureidoglycolate | mutant H44A, pH 8.1, 30°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-ureidoglycolate + NAD+ | - |
Escherichia coli | oxalureate + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | gel filtration | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
(S)-ureidoglycolate dehydrogenase | - |
Escherichia coli |
AllD | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
NAD+ | mutant S140A, pH 8.1, 30°C | Escherichia coli | |
0.87 | - |
NAD+ | mutant D141E, pH 8.1, 30°C | Escherichia coli | |
1.03 | - |
NAD+ | mutant H44A, pH 8.1, 30°C | Escherichia coli | |
1.06 | - |
NAD+ | mutant D141A, pH 8.1, 30°C | Escherichia coli | |
1.29 | - |
(S)-ureidoglycolate | mutant S140A, pH 8.1, 30°C | Escherichia coli | |
1.37 | - |
(S)-ureidoglycolate | mutant S43A, pH 8.1, 30°C | Escherichia coli | |
1.47 | - |
NAD+ | mutant D141N, pH 8.1, 30°C | Escherichia coli | |
1.62 | - |
(S)-ureidoglycolate | mutant D141A, pH 8.1, 30°C | Escherichia coli | |
1.67 | - |
(S)-ureidoglycolate | mutant D141E, pH 8.1, 30°C | Escherichia coli | |
1.76 | - |
(S)-ureidoglycolate | mutant H44A, pH 8.1, 30°C | Escherichia coli | |
1.9 | - |
NAD+ | mutant Y52F, pH 8.1, 30°C | Escherichia coli | |
1.92 | - |
(S)-ureidoglycolate | mutant Y52F, pH 8.1, 30°C | Escherichia coli | |
1.97 | - |
(S)-ureidoglycolate | mutant D141N, pH 8.1, 30°C | Escherichia coli | |
2.51 | - |
NAD+ | mutant S43A, pH 8.1, 30°C | Escherichia coli | |
9.29 | - |
NAD+ | mutant R259A, pH 8.1, 30°C | Escherichia coli | |
11.98 | - |
(S)-ureidoglycolate | mutant R259A, pH 8.1, 30°C | Escherichia coli | |
30.65 | - |
NAD+ | mutant M251A, pH 8.1, 30°C | Escherichia coli | |
45.98 | - |
(S)-ureidoglycolate | mutant M251A, pH 8.1, 30°C | Escherichia coli | |
57 | - |
(S)-ureidoglycolate | wild-type, pH 8.1, 30°C | Escherichia coli | |
62 | - |
NAD+ | wild-type, pH 8.1, 30°C | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.1 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
NAD+ | mutant S140A, pH 8.1, 30°C | Escherichia coli | |
0.1 | - |
(S)-ureidoglycolate | mutant H44A, pH 8.1, 30°C | Escherichia coli | |
0.1 | - |
(S)-ureidoglycolate | mutant S140A, pH 8.1, 30°C | Escherichia coli | |
0.11 | - |
(S)-ureidoglycolate | mutant Y52F, pH 8.1, 30°C | Escherichia coli | |
0.12 | - |
(S)-ureidoglycolate | mutant D141E, pH 8.1, 30°C | Escherichia coli | |
0.13 | - |
(S)-ureidoglycolate | mutant S43A, pH 8.1, 30°C | Escherichia coli | |
0.31 | - |
(S)-ureidoglycolate | mutant D141A, pH 8.1, 30°C | Escherichia coli | |
0.34 | - |
(S)-ureidoglycolate | mutant D141N, pH 8.1, 30°C | Escherichia coli | |
1.04 | - |
(S)-ureidoglycolate | mutant R259A, pH 8.1, 30°C | Escherichia coli | |
1.1 | - |
NAD+ | mutant S43A, pH 8.1, 30°C | Escherichia coli | |
1.51 | - |
NAD+ | mutant Y52F, pH 8.1, 30°C | Escherichia coli | |
1.6 | - |
NAD+ | mutant H44A, pH 8.1, 30°C | Escherichia coli | |
1.81 | - |
NAD+ | mutant D141N, pH 8.1, 30°C | Escherichia coli | |
2.04 | - |
NAD+ | mutant D141A, pH 8.1, 30°C | Escherichia coli | |
2.35 | - |
NAD+ | mutant D141E, pH 8.1, 30°C | Escherichia coli | |
3.49 | - |
(S)-ureidoglycolate | mutant M251A, pH 8.1, 30°C | Escherichia coli | |
6.23 | - |
NAD+ | mutant R259A, pH 8.1, 30°C | Escherichia coli | |
32.9 | - |
NAD+ | mutant M251A, pH 8.1, 30°C | Escherichia coli | |
54 | - |
(S)-ureidoglycolate | wild-type, pH 8.1, 30°C | Escherichia coli | |
110 | - |
NAD+ | wild-type, pH 8.1, 30°C | Escherichia coli |