Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.154 extracted from

  • Rothe, M.; Alpert, C.; Loh, G.; Blaut, M.
    Novel insights into E. colis hexuronate metabolism: KduI facilitates the conversion of galacturonate and glucuronate under osmotic stress conditions (2013), PLoS ONE, 8, e56906.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expresed as a His-tagged fusion protein in Escherichia coli Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure of AllD in its apo form is determined at 2.13 A resolution, as well as a binary complex at 1.64 A resolution with the NADH cofactor, and a ternary complex at 1.77 A resolution with NADH and glyoxylate, a product yielded from the spontaneous degradation of oxalurate Escherichia coli

Protein Variants

Protein Variants Comment Organism
D141A Km ((S)-ureidoglycolate) increased, kcat (S-ureidoglycolate) or (NAD+) decreased compared to wild-type, Km (NAD+) similar to wo wild-type Escherichia coli
D141E Km ((S)-ureidoglycolate) increased, kcat ((S)-ureidoglycolate) or (NAD+) decreased compared to wild-type, Km (NAD+) decreased compared to wild-type Escherichia coli
D141N Km ((S)-ureidoglycolate) or (NAD+) increased, kcat ((S)-ureidoglycolate) or (NAD+) decreased compared to wild-type Escherichia coli
H116A mutant(S)-ureidoglycolate shows no activity Escherichia coli
H44A Km ((S)-ureidoglycolate) increased, kcat (S-ureidoglycolate) or (NAD+) decreased compared to wild-type, Km (NAD+) equal to wild-type Escherichia coli
M251A Km ((S)-ureidoglycolate) or (NAD+) increased, kcat ((S)-ureidoglycolate) or (NAD+) decreased compared to wild-type Escherichia coli
R259A Km ((S)-ureidoglycolate) or (NAD+) increased, kcat ((S)-ureidoglycolate) or (NAD+) decreased compared to wild-type Escherichia coli
R48A Km ((S)-ureidoglycolate) or (NAD+) increased, kcat ((S)-ureidoglycolate) or (NAD+) decreased compared to wild-type Escherichia coli
S140A Km ((S)-ureidoglycolate) or (NAD+) increased, kcat ((S)-ureidoglycolate) or (NAD+) decreased compared to wild-type Escherichia coli
S43A Km ((S)-ureidoglycolate) or (NAD+) increased, kcat ((S)-ureidoglycolate) or (NAD+) decreased compared to wild-type Escherichia coli
Y52F Km ((S)-ureidoglycolate) or (NAD+) increased, kcat ((S)-ureidoglycolate) or (NAD+) decreased compared to wild-type Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.37
-
NAD+ mutant D141E, pH 8.1, 30°C Escherichia coli
0.52
-
NAD+ mutant D141A, pH 8.1, 30°C Escherichia coli
0.56
-
NAD+ wild-type, pH 8.1, 30°C Escherichia coli
0.65
-
NAD+ mutant H44A, pH 8.1, 30°C Escherichia coli
0.81
-
NAD+ mutant D141N, pH 8.1, 30°C Escherichia coli
0.93
-
NAD+ mutant M251A, pH 8.1, 30°C Escherichia coli
1.06
-
(S)-ureidoglycolate wild-type, pH 8.1, 30°C Escherichia coli
1.26
-
NAD+ mutant Y52F, pH 8.1, 30°C Escherichia coli
1.39
-
NAD+ mutant S140A, pH 8.1, 30°C Escherichia coli
1.49
-
NAD+ mutant R259A, pH 8.1, 30°C Escherichia coli
2.28
-
NAD+ mutant S43A, pH 8.1, 30°C Escherichia coli
5.27
-
(S)-ureidoglycolate mutant D141A, pH 8.1, 30°C Escherichia coli
5.87
-
(S)-ureidoglycolate mutant D141N, pH 8.1, 30°C Escherichia coli
10.75
-
(S)-ureidoglycolate mutant S43A, pH 8.1, 30°C Escherichia coli
11.47
-
(S)-ureidoglycolate mutant R259A, pH 8.1, 30°C Escherichia coli
12.38
-
(S)-ureidoglycolate mutant S140A, pH 8.1, 30°C Escherichia coli
13.15
-
(S)-ureidoglycolate mutant M251A, pH 8.1, 30°C Escherichia coli
14.13
-
(S)-ureidoglycolate mutant D141E, pH 8.1, 30°C Escherichia coli
16.82
-
(S)-ureidoglycolate mutant Y52F, pH 8.1, 30°C Escherichia coli
16.94
-
(S)-ureidoglycolate mutant H44A, pH 8.1, 30°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-ureidoglycolate + NAD+
-
Escherichia coli oxalureate + NADH
-
?

Subunits

Subunits Comment Organism
dimer gel filtration Escherichia coli

Synonyms

Synonyms Comment Organism
(S)-ureidoglycolate dehydrogenase
-
Escherichia coli
AllD
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.02
-
NAD+ mutant S140A, pH 8.1, 30°C Escherichia coli
0.87
-
NAD+ mutant D141E, pH 8.1, 30°C Escherichia coli
1.03
-
NAD+ mutant H44A, pH 8.1, 30°C Escherichia coli
1.06
-
NAD+ mutant D141A, pH 8.1, 30°C Escherichia coli
1.29
-
(S)-ureidoglycolate mutant S140A, pH 8.1, 30°C Escherichia coli
1.37
-
(S)-ureidoglycolate mutant S43A, pH 8.1, 30°C Escherichia coli
1.47
-
NAD+ mutant D141N, pH 8.1, 30°C Escherichia coli
1.62
-
(S)-ureidoglycolate mutant D141A, pH 8.1, 30°C Escherichia coli
1.67
-
(S)-ureidoglycolate mutant D141E, pH 8.1, 30°C Escherichia coli
1.76
-
(S)-ureidoglycolate mutant H44A, pH 8.1, 30°C Escherichia coli
1.9
-
NAD+ mutant Y52F, pH 8.1, 30°C Escherichia coli
1.92
-
(S)-ureidoglycolate mutant Y52F, pH 8.1, 30°C Escherichia coli
1.97
-
(S)-ureidoglycolate mutant D141N, pH 8.1, 30°C Escherichia coli
2.51
-
NAD+ mutant S43A, pH 8.1, 30°C Escherichia coli
9.29
-
NAD+ mutant R259A, pH 8.1, 30°C Escherichia coli
11.98
-
(S)-ureidoglycolate mutant R259A, pH 8.1, 30°C Escherichia coli
30.65
-
NAD+ mutant M251A, pH 8.1, 30°C Escherichia coli
45.98
-
(S)-ureidoglycolate mutant M251A, pH 8.1, 30°C Escherichia coli
57
-
(S)-ureidoglycolate wild-type, pH 8.1, 30°C Escherichia coli
62
-
NAD+ wild-type, pH 8.1, 30°C Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.1
-
assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NADH
-
Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.01
-
NAD+ mutant S140A, pH 8.1, 30°C Escherichia coli
0.1
-
(S)-ureidoglycolate mutant H44A, pH 8.1, 30°C Escherichia coli
0.1
-
(S)-ureidoglycolate mutant S140A, pH 8.1, 30°C Escherichia coli
0.11
-
(S)-ureidoglycolate mutant Y52F, pH 8.1, 30°C Escherichia coli
0.12
-
(S)-ureidoglycolate mutant D141E, pH 8.1, 30°C Escherichia coli
0.13
-
(S)-ureidoglycolate mutant S43A, pH 8.1, 30°C Escherichia coli
0.31
-
(S)-ureidoglycolate mutant D141A, pH 8.1, 30°C Escherichia coli
0.34
-
(S)-ureidoglycolate mutant D141N, pH 8.1, 30°C Escherichia coli
1.04
-
(S)-ureidoglycolate mutant R259A, pH 8.1, 30°C Escherichia coli
1.1
-
NAD+ mutant S43A, pH 8.1, 30°C Escherichia coli
1.51
-
NAD+ mutant Y52F, pH 8.1, 30°C Escherichia coli
1.6
-
NAD+ mutant H44A, pH 8.1, 30°C Escherichia coli
1.81
-
NAD+ mutant D141N, pH 8.1, 30°C Escherichia coli
2.04
-
NAD+ mutant D141A, pH 8.1, 30°C Escherichia coli
2.35
-
NAD+ mutant D141E, pH 8.1, 30°C Escherichia coli
3.49
-
(S)-ureidoglycolate mutant M251A, pH 8.1, 30°C Escherichia coli
6.23
-
NAD+ mutant R259A, pH 8.1, 30°C Escherichia coli
32.9
-
NAD+ mutant M251A, pH 8.1, 30°C Escherichia coli
54
-
(S)-ureidoglycolate wild-type, pH 8.1, 30°C Escherichia coli
110
-
NAD+ wild-type, pH 8.1, 30°C Escherichia coli