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Literature summary for 1.1.1.150 extracted from

  • Monder, C.; White, A.
    The 21-hydroxysteroid dehydrogenases of liver. A nicotinamide adenine dinucleotide phosphate dehydrogenase and two nicotinamide adenine dinucleotide dehydrogenases (1965), J. Biol. Chem., 240, 71-77.
    View publication on PubMed
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Inhibitors

Inhibitors Comment Organism Structure
Ag+ complete inhibition of NAD dehydrogenase II at 0.018 mM Ovis aries aries
Hg2+ 40% inhibition of NAD dehydrogenase II at 0.004 mM Ovis aries aries
NAD+ NAD dehydrogenase II, competitive inhibition Ovis aries aries
NADP+ NAD dehydrogenase II, competitive inhibition Ovis aries aries
p-mercuribenzoate 79% inhibition of NAD dehydrogenase II at 0.014 mM Ovis aries aries

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 NADH NAD dehydrogenase I Ovis aries aries
0.048
-
 NADH NAD dehydrogenase II Ovis aries aries
0.105
-
 11beta-hydroxy-pregn-4-ene-3,20,21-trione NAD dehydrogenase II Ovis aries aries
0.111
-
 17-hydroxy-pregn-4-ene-3,20,21-trione NAD dehydrogenase II Ovis aries aries
0.172
-
 11beta,17-dihydroxy-pregn-4,11-diene-3,20,21-trione NAD dehydrogenase II Ovis aries aries
0.174
-
 pregn-4-ene-3,20,21-trione NAD dehydrogenase II Ovis aries aries
0.182
-
 11beta,17-dihydroxy-pregn-4-ene-3,20,21-trione
-
 Ovis aries aries
0.221
-
 17-hydroxy-pregn-4-ene-3,11,20,21-tetraone
-
 Ovis aries aries

Organism

Organism UniProt Comment Textmining
Ovis aries aries
-
 sheep
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, gel filtration, starch block electrophoresis Ovis aries aries

Source Tissue

Source Tissue Comment Organism Textmining
liver 2 isoenzymes: NAD dehydrogenase I and II Ovis aries aries
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
11beta,17-dihydroxy-pregn-4,11-diene-3,20,21-trione + NADH i.e. 21-dehydro-delta1-cortisol, only NAD dehydrogenase II Ovis aries aries 11beta,17,21-trihydroxy-pregn-4,11-diene-3,20-dione + NAD+ i.e. 21-hydroxy-delta1-cortisol ?
11beta,17-dihydroxy-pregn-4-ene-3,20,21-trione + NADH
-
 Ovis aries aries 11beta,17,21-trihydroxy-pregn-4-ene-3,20-dione + NAD+ i.e. cortisol ?
11beta-hydroxy-pregn-4-ene-3,20,21-trione + NADH i.e. 21-dehydrocorticosterone, only NAD dehydrogenase II Ovis aries aries 11beta,21-dihydroxy-pregn-4-ene-3,20-dione + NAD+ i.e. 21-hydroxycorticosterone ?
17-hydroxy-pregn-4-ene-3,11,20,21-tetraone + NADH i.e. 21-dehydrocortisone Ovis aries aries 17,21-dihydroxy-pregn-4-ene-3,11,20-trione + NAD+ i.e. cortisone ?
17-hydroxy-pregn-4-ene-3,20,21-trione + NADH i.e. 21-dehydro-11-deoxycortisol, only NAD dehydrogenase II Ovis aries aries 17,21-dihydroxy-pregn-4-ene-3,20-dione + NAD+ i.e. 21-hydroxy-11-deoxycortisol ?
pregn-4-ene-3,20,21-trione + NADH i.e. 21-dehydro-11-deoxycorticosterone, only NAD dehydrogenase II Ovis aries aries 21-hydroxy-pregn-4-ene-3,20-dione + NAD+ i.e. 21-hydroxy-11-deoxycorticosterone ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
56
-
 30% loss of activity in 5 min, NAD dehydrogenase I more heat resistant than NAD dehydrogenase II Ovis aries aries

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
 NAD dehydrogenase II Ovis aries aries
6.9
-
 NAD dehydrogenase I Ovis aries aries

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Ovis aries aries