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Literature summary for 1.1.1.145 extracted from

  • Thomas, J.L.; Mason, J.I.; Brandt, S.; Norris, W.
    Differences in substrate and inhibitor kinetics of human type 1 and type 2 3beta-hydroxysteroid dehydrogenase are explained by the type 1 mutant, H156Y (2002), Endocr. Res., 28, 471-475.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Sf9 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
H156Y 195% of wild-type activity Homo sapiens
K158Q no activity Homo sapiens
Y154F no activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
epostane
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0037
-
dehydroepiandrosterone pH 7.4 Homo sapiens
0.042
-
dehydroepiandrosterone pH 7.4, H156Y mutant Homo sapiens
0.047
-
dehydroepiandrosterone 27°C, pH 7.4 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dehydroepiandrosterone + NAD+ Homo sapiens
-
androst-5-en-3,17-dione + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant 3beta-HSD Homo sapiens
recombinant 3beta-HSD type 2 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
adrenal gland
-
Homo sapiens
-
gonad
-
Homo sapiens
-
mammary gland
-
Homo sapiens
-
placenta
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dehydroepiandrosterone + NAD+
-
Homo sapiens androst-5-en-3,17-dione + NADH + H+
-
?

Synonyms

Synonyms Comment Organism
3beta-hydroxysteroid dehydrogenase type 1
-
Homo sapiens
3beta-hydroxysteroid dehydrogenase type 2
-
Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000007
-
epostane 27°C, pH 7.4 Homo sapiens
0.000098
-
epostane 27°C, pH 7.4 Homo sapiens