Literature summary for 1.1.1.145 extracted from

Finsterbusch, A.; Lindemann, P.; Grimm, R.; Eckerskorn, C.; Luckner, M.
DELTA5-3beta-Hydroxysteroid dehydrogenase from Digitalis lanata Ehrh. - a multifunctional enzyme in steroid metabolism? (1999), Planta, 209, 478-486.

Search for more literature for 1.1.1.145

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.02	-	pregnenolone	NAD+ as cofactor	Digitalis lanata
0.11	-	5beta-pregnane-3beta-ol-20-one	NAD+ as cofactor	Digitalis lanata
0.12	-	5alpha-pregnane-3beta-ol-20-one	NAD+ as cofactor	Digitalis lanata
0.13	-	androst-5-ene-3beta-ol-17-one	NAD+ as cofactor	Digitalis lanata

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
29000	-	x * 29000, SDS-PAGE	Digitalis lanata

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
pregnenolone + NAD(P)+	Digitalis lanata	-	pregn-5-en-3,20-dione + NAD(P)H + H+	-	ir
pregnenolone + NAD(P)+	Digitalis lanata L.	-	pregn-5-en-3,20-dione + NAD(P)H + H+	-	ir

Organism

Organism	UniProt	Comment	Textmining
Digitalis lanata	-	L.	-
Digitalis lanata L.	-	L.	-

Purification (Commentary)

Purification (Comment)	Organism
-	Digitalis lanata

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	-	Digitalis lanata	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.0016	-	-	Digitalis lanata

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5alpha-pregnane-3,20-dione + NAD(P)H	-	Digitalis lanata	5alpha-pregnane-3beta-ol-20-one + NAD(P)+	-	r
5alpha-pregnane-3,20-dione + NAD(P)H	-	Digitalis lanata L.	5alpha-pregnane-3beta-ol-20-one + NAD(P)+	-	r
5beta-pregnane-3,20-dione + NAD(P)H + H+	-	Digitalis lanata	5beta-pregnane-3beta-ol-20-one + NAD(P)+	-	r
5beta-pregnane-3,20-dione + NAD(P)H + H+	-	Digitalis lanata L.	5beta-pregnane-3beta-ol-20-one + NAD(P)+	-	r
androst-5-ene-3beta-ol-17-one + NAD+	-	Digitalis lanata	androst-5-ene-3,17-dione + NADH	-	ir
androst-5-ene-3beta-ol-17-one + NAD+	-	Digitalis lanata L.	androst-5-ene-3,17-dione + NADH	-	ir
pregnenolone + NAD(P)+	-	Digitalis lanata	pregn-5-en-3,20-dione + NAD(P)H + H+	-	ir
pregnenolone + NAD(P)+	-	Digitalis lanata L.	pregn-5-en-3,20-dione + NAD(P)H + H+	-	ir

Subunits

Subunits	Comment	Organism
?	x * 29000, SDS-PAGE	Digitalis lanata

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Digitalis lanata
NADP+	-	Digitalis lanata
NADPH	-	Digitalis lanata

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Release 2023.1 (January 2023)