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Literature summary for 1.1.1.145 extracted from

  • Thomas, J.L.; Mason, J.I.; Blanco, G.; Veisaga, M.L.
    The engineered, cytosolic form of human type I 3beta-hydroxysteroid dehydrogenase/isomerase: purification, characterization and crystallization (2001), J. Mol. Endocrinol., 27, 77-83.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression with his6-tag, using a baculovirus expression system Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
modified enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0034
-
NAD+ wild type enzyme Homo sapiens
0.0035
-
NAD+ modified enzyme Homo sapiens
0.0037
-
dehydroepiandrosterone wild type enzyme Homo sapiens
0.0045
-
dehydroepiandrosterone modified enzyme Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
42000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pregnenolone + NAD(P)+ Homo sapiens
-
pregn-5-en-3,20-dione + NAD(P)H + H+
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
3beta-HSD I
-

Source Tissue

Source Tissue Comment Organism Textmining
placenta 3beta-HSD I Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0524
-
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3beta,17alpha-dihydroxy-5-pregnen-20-one + NAD(P)+
-
Homo sapiens 17alpha-hydroxypregn-5-ene-3,20-dione + NAD(P)H
-
?
pregnenolone + NAD(P)+
-
Homo sapiens pregn-5-en-3,20-dione + NAD(P)H + H+
-
?

Subunits

Subunits Comment Organism
? 42000, SDS-PAGE Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Homo sapiens