Literature summary for 1.1.1.138 extracted from

Simon-Nobbe, B.; Denk, U.; Schneider, P.B.; Radauer, C.; Teige, M.; Crameri, R.; Hawranek, T.; Lang, R.; Richter, K.; Schmid-Grendelmeier, P.; Nobbe, S.; Hartl, A.; Breitenbach, M.
NADP-dependent mannitol dehydrogenase, a major allergen of Cladosporium herbarum (2006), J. Biol. Chem., 281, 16354-16360.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in E. coli	Cladosporium herbarum
gene MtDH, DNA and amino acid sequence determination and analysis, expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)	Cladosporium herbarum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.053	-	NADP+	pH 7.5, 22°C	Cladosporium herbarum
0.053	-	NADPH	plusminus 0.01 mM, pH 7.5, room temperature	Cladosporium herbarum
0.067	-	NADPH	pH 7.5, 22°C	Cladosporium herbarum
0.067	-	NADP+	plusminus 0.007 mM, pH 7.5, room temperature	Cladosporium herbarum
33	-	D-mannitol	plusminus 5 mM, pH 7.5, room temperature	Cladosporium herbarum
117	-	D-fructose	plusminus 20 mM, pH 7.5, room temperature	Cladosporium herbarum
230	-	D-mannitol	pH 7.5, 22°C	Cladosporium herbarum
1170	-	D-fructose	pH 7.5, 22°C	Cladosporium herbarum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
28320	-	-	Cladosporium herbarum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-mannitol + NADP+	Cladosporium herbarum	-	D-fructose + NADPH + H+	-	r
additional information	Cladosporium herbarum	the enzyme is the major allergen in the organism causing allergic reaction e.g. in humans	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Cladosporium herbarum	P0C0Y5	-	-
Cladosporium herbarum	P0C0Y5	i.e. Davidiella tassiana, strain 280202	-

Purification (Commentary)

Purification (Comment)	Organism
native enzyme 13.4fold by ammonium sulfate fractionation, hydrophobic interaction chromatography, and anion exchange chromatography	Cladosporium herbarum
purified by standard chromatographic methods	Cladosporium herbarum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	allergenic potential on human skin, overview	Cladosporium herbarum
277	-	purified native enzyme	Cladosporium herbarum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-fructose + NADPH + H+	-	Cladosporium herbarum	D-mannitol + NADP+	-	r
D-mannitol + NADP+	-	Cladosporium herbarum	D-fructose + NADPH + H+	-	r
additional information	the enzyme is the major allergen in the organism causing allergic reaction e.g. in humans	Cladosporium herbarum	?	-	?

Synonyms

Synonyms	Comment	Organism
Cla h 8	-	Cladosporium herbarum
Cla h 8 allergen	-	Cladosporium herbarum
MtDH	-	Cladosporium herbarum
NADP-dependent mannitol dehydrogenase	-	Cladosporium herbarum
NADP-dependent MtDH	-	Cladosporium herbarum

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
22	-	assay at room temperature, forward reaction	Cladosporium herbarum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at, forward reaction	Cladosporium herbarum

Cofactor

Cofactor	Comment	Organism	Structure
NADP+	-	Cladosporium herbarum
NADPH	-	Cladosporium herbarum

pI Value

Organism	Comment	pI Value Maximum	pI Value
Cladosporium herbarum	-	-	5.8

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Release 2023.1 (January 2023)