Application | Comment | Organism |
---|---|---|
analysis | determination of D-fructose in the presence of other sugars | Gluconobacter oxydans |
Crystallization (Comment) | Organism |
---|---|
ammonium sulfate precipitation | Gluconobacter oxydans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.019 | - |
NADPH | - |
Gluconobacter oxydans | |
0.025 | - |
NADP+ | - |
Gluconobacter oxydans | |
10 | - |
D-mannitol | - |
Gluconobacter oxydans | |
12 | - |
D-fructose | - |
Gluconobacter oxydans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
gel filtration | Gluconobacter oxydans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose + NADPH | Gluconobacter oxydans | - |
D-mannitol + NADP+ | - |
? | |
D-fructose + NADPH | Gluconobacter oxydans IFO 12528 | - |
D-mannitol + NADP+ | - |
? | |
D-mannitol + NADP+ | Gluconobacter oxydans | - |
D-fructose + NADPH + H+ | - |
r | |
D-mannitol + NADP+ | Gluconobacter oxydans IFO 12528 | - |
D-fructose + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconobacter oxydans | - |
- |
- |
Gluconobacter oxydans IFO 12528 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to crystalline state, chromatography techniques, crystallization | Gluconobacter oxydans |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Gluconobacter oxydans | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
235 | - |
- |
Gluconobacter oxydans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose + NADPH | - |
Gluconobacter oxydans | D-mannitol + NADP+ | - |
? | |
D-fructose + NADPH | - |
Gluconobacter oxydans IFO 12528 | D-mannitol + NADP+ | - |
? | |
D-mannitol + NADP+ | - |
Gluconobacter oxydans | D-fructose + NADPH + H+ | - |
r | |
D-mannitol + NADP+ | - |
Gluconobacter oxydans IFO 12528 | D-fructose + NADPH + H+ | - |
r | |
L-sorbitol + NADP+ | 4% of the activity compared to D-mannitol | Gluconobacter oxydans | L-sorbose + NADPH | - |
r | |
L-sorbitol + NADP+ | 4% of the activity compared to D-mannitol | Gluconobacter oxydans IFO 12528 | L-sorbose + NADPH | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 50000, SDS-PAGE | Gluconobacter oxydans |
Synonyms | Comment | Organism |
---|---|---|
D-mannitol dehydrogenase, NADP-dependent | - |
Gluconobacter oxydans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
stable over 30 min | Gluconobacter oxydans |
53 | - |
20-50% retaining activity after 20 min | Gluconobacter oxydans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | reduction of D-fructose | Gluconobacter oxydans |
9 | 10 | oxidation of D-mannitol | Gluconobacter oxydans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.5 | 9.5 | stable within | Gluconobacter oxydans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | specific for NADP+ | Gluconobacter oxydans | |
NADPH | - |
Gluconobacter oxydans |