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Literature summary for 1.1.1.138 extracted from

  • Stoop, J.M.H.; Mooibroek, H.
    Cloning and characterization of NADP-dependent dehydrogenase cDNA from the button mushroom, Agaricus bisporus, and its expression in response to NaCl stress (1998), Appl. Environ. Microbiol., 64, 4689-4696.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris, gene cloning in Escherichia coli Agaricus bisporus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 x * 29000, SDS-PAGE Agaricus bisporus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-mannitol + NADP+ Agaricus bisporus
-
 D-fructose + NADPH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Agaricus bisporus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques Agaricus bisporus

Source Tissue

Source Tissue Comment Organism Textmining
fruit body stage 2, highest activity in salt stressed fruit bodies Agaricus bisporus
-
mycelium
-
 Agaricus bisporus
-

Storage Stability

Storage Stability Organism
-80°C, 50 mM MOPS, pH 7.5, 1 mM DTT, 50 mM NaCl, several weeks Agaricus bisporus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-mannitol + NADP+
-
 Agaricus bisporus D-fructose + NADPH + H+
-
 r

Subunits

Subunits Comment Organism
? x * 29000, SDS-PAGE Agaricus bisporus

Synonyms

Synonyms Comment Organism
NADP-mannitol dehydrogenase
-
 Agaricus bisporus

Cofactor

Cofactor Comment Organism Structure
NADP+ specific for NADP+ Agaricus bisporus