Cloned (Comment) | Organism |
---|---|
overexpressed in Escherichia coli BL21(lambdaDE3) | Salmonella enterica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | up to 0.3 mM, 70% loss of enzyme activity | Salmonella enterica | |
additional information | full activity restored with up to 0.3 mM MgCl2 | Salmonella enterica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.106 | - |
NADH | +/-0.018, pH 7.0 | Salmonella enterica | |
0.21 | - |
NADH | +/-0.004, pH 7.0 | Salmonella enterica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32600 | - |
calculated from nucleotide sequence data | Salmonella enterica |
34800 | - |
SDS-PAGE | Salmonella enterica |
41500 | - |
gel filtration | Salmonella enterica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica | - |
serovar typhimurium LT2 | - |
Purification (Comment) | Organism |
---|---|
affinity and ion exchange chromatography | Salmonella enterica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
44 | - |
- |
Salmonella enterica |
Storage Stability | Organism |
---|---|
-20°C, 44% glycerol | Salmonella enterica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-4-dehydro-6-deoxy-D-glucose + NADH + H+ | - |
Salmonella enterica | dTDP-beta-L-rhamnose + NAD+ | - |
? | |
dTDP-6-deoxy-L-lyxo-4-hexulose + NAD(P)H | - |
Salmonella enterica | dTDP-L-rhamnose + NAD(P)+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
dTDP-4-dehydrorhamnose reductase | - |
Salmonella enterica |
thymidine diphosphate-4-dehydrorhamnose reductase | - |
Salmonella enterica |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
stable below | Salmonella enterica |
37 | - |
pH 7.0, 75% activity loss | Salmonella enterica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
25 | - |
NADH | +/-48 | Salmonella enterica | |
29 | - |
NADPH | +/-66 | Salmonella enterica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
reduction of dTDP-6-deoxy-L-lyxo-4-hexulose | Salmonella enterica |
10 | - |
oxidation of dTDP-L-rhamnose | Salmonella enterica |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
37°C, 20 min, 75% activity loss | Salmonella enterica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Salmonella enterica | |
NADH | preferred | Salmonella enterica | |
NADP+ | - |
Salmonella enterica | |
NADPH | - |
Salmonella enterica |