Cloned (Comment) | Organism |
---|---|
native overexpressed in Escherichia coli BL21(DE3) and selenomethionine-enriched in B834(lambdaDE3) | Salmonella enterica |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapour-diffusion method, 6 d | Salmonella enterica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32500 | - |
HPLC | Salmonella enterica |
32540 | - |
mass spectrometry, native protein | Salmonella enterica |
32560 | - |
deduced from sequence | Salmonella enterica |
32730 | - |
mass spectrometry, selenomethionine-enriched protein | Salmonella enterica |
49500 | - |
light-scattering | Salmonella enterica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH | Escherichia coli | synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria | dTDP-L-rhamnose + NADP+ | - |
? | |
dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH | Xanthomonas campestris | synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria | dTDP-L-rhamnose + NADP+ | - |
? | |
dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH | Salmonella enterica | synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria | dTDP-L-rhamnose + NADP+ | - |
? | |
dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH | Shigella flexneri | synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria | dTDP-L-rhamnose + NADP+ | - |
? | |
dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH | Streptomyces mutans | synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria | dTDP-L-rhamnose + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
K12 | - |
Salmonella enterica | - |
- |
- |
Shigella flexneri | - |
- |
- |
Streptomyces mutans | - |
- |
- |
Xanthomonas campestris | - |
- |
- |
Purification (Comment) | Organism |
---|---|
anion-exchange, hydrophobic chromatography | Salmonella enterica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH | synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria | Escherichia coli | dTDP-L-rhamnose + NADP+ | - |
? | |
dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH | synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria | Xanthomonas campestris | dTDP-L-rhamnose + NADP+ | - |
? | |
dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH | synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria | Salmonella enterica | dTDP-L-rhamnose + NADP+ | - |
? | |
dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH | synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria | Shigella flexneri | dTDP-L-rhamnose + NADP+ | - |
? | |
dTDP-6-deoxy-L-lyxo-4-hexulose + NADPH | synthesis of L-rhamnose precurser dTDP-L-rhamnose, essential cell wall component of pathogenic bacteria | Streptomyces mutans | dTDP-L-rhamnose + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dTDP-6-deoxy-L-lyxo-4-hexulose reductase | - |
Salmonella enterica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Escherichia coli | |
NADPH | - |
Xanthomonas campestris | |
NADPH | - |
Salmonella enterica | |
NADPH | - |
Shigella flexneri | |
NADPH | - |
Streptomyces mutans |