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Literature summary for 1.1.1.122 extracted from

  • Endo, M.; Hiyama, N.
    Isolation and characterization of L-fucose dehydrogenase from rabbit liver (1979), J. Biochem., 86, 1559-1565.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ inhibitory Oryctolagus cuniculus
Hg2+
-
Oryctolagus cuniculus
iodoacetic acid 10% inhibition at 1 mM Oryctolagus cuniculus
p-chloromercuribenzoate 48% inhibition at 1 mM Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
NAD+
-
Oryctolagus cuniculus
0.15
-
6-deoxy-L-galactose
-
Oryctolagus cuniculus
1.4
-
D-arabinose
-
Oryctolagus cuniculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
92000
-
gel filtration Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6-deoxy-L-galactose + NAD+ Oryctolagus cuniculus irreversible because of spontaneous conversion to L-fuconic acid during incubation 6-deoxy-L-galactono-1,5-lactone + NADH
-
ir
6-deoxy-L-galactose + NAD+ Oryctolagus cuniculus i.e. L-fucose 6-deoxy-L-galactono-1,5-lactone + NADH
-
ir
D-arabinose + NAD+ Oryctolagus cuniculus 84% of the activity compared to L-fucose D-arabinono-1,4-lactone + NADH
-
?
D-xylose + NAD+ Oryctolagus cuniculus 2.1% of the activity compared to L-fucose D-xylono-1,5-lactone + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
rabbit
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography steps, preparative gel electrophoresis, affinity chromatography Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
1.03
-
purified enzyme Oryctolagus cuniculus

Storage Stability

Storage Stability Organism
-20┬░C, presence of NAD+, 1 year Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-deoxy-L-galactose + NAD+ irreversible because of spontaneous conversion to L-fuconic acid during incubation Oryctolagus cuniculus 6-deoxy-L-galactono-1,5-lactone + NADH
-
ir
6-deoxy-L-galactose + NAD+ i.e. L-fucose Oryctolagus cuniculus 6-deoxy-L-galactono-1,5-lactone + NADH
-
ir
D-arabinose + NAD+ 84% of the activity compared to L-fucose Oryctolagus cuniculus D-arabinono-1,4-lactone + NADH
-
?
D-xylose + NAD+ 2.1% of the activity compared to L-fucose Oryctolagus cuniculus D-xylono-1,5-lactone + NADH + H+
-
?

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
50
-
-
Oryctolagus cuniculus

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
50
-
half-life: 30 min Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
Oryctolagus cuniculus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 12 stable between Oryctolagus cuniculus

Cofactor

Cofactor Comment Organism Structure
NAD+ NAD-specific Oryctolagus cuniculus