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Literature summary for 1.1.1.122 extracted from

  • Sasajima, K.I.; Sinskey, A.J.
    Oxidation of L-glucose by a pseudomonad (1979), Biochim. Biophys. Acta, 571, 120-126.
    View publication on PubMed

General Stability

General Stability Organism
crude enzyme preparations stable, purified enzyme very unstable Trinickia caryophylli

Inhibitors

Inhibitors Comment Organism Structure
D-galactose 79% inhibition of L-glucose oxidation Trinickia caryophylli
D-glucose competitive inhibition of L-glucose oxidation, Ki: 130 mM Trinickia caryophylli
L-arabinose 74% inhibition of L-glucose oxidation Trinickia caryophylli
L-rhamnose 74% inhibition of L-glucose oxidation Trinickia caryophylli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1
-
6-deoxy-L-galactose
-
Trinickia caryophylli
2.8
-
D-arabinose
-
Trinickia caryophylli
4.5
-
L-Xylose
-
Trinickia caryophylli
15
-
L-Glucose
-
Trinickia caryophylli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
71000
-
gel filtration Trinickia caryophylli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6-deoxy-L-galactose + NAD+ Trinickia caryophylli 73% of the activity compared to D-arabinose or L-xylose 6-deoxy-L-galactono-1,5-lactone + NADH
-
?
6-deoxy-L-galactose + NAD+ Trinickia caryophylli i.e. L-fucose 6-deoxy-L-galactono-1,5-lactone + NADH
-
?
D-arabinose + NAD+ Trinickia caryophylli 100% activity D-arabinono-1,4-lactone + NADH
-
?
D-ribose + NAD+ Trinickia caryophylli 8% of the activity compared to D-arabinose or L-xylose ? + NADH
-
?
D-xylose + NAD+ Trinickia caryophylli 9% of the activity compared to D-arabinose or L-xylose D-xylono-1,5-lactone + NADH + H+
-
?
L-glucose + NAD+ Trinickia caryophylli 68% of the activity compared to D-arabinose or L-xylose L-glucono-1,5-lactone + NADH
-
?
L-xylose + NAD+ Trinickia caryophylli 100% activity L-xylono-1,5-lactone + NADH
-
?

Organism

Organism UniProt Comment Textmining
Trinickia caryophylli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques Trinickia caryophylli

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Trinickia caryophylli
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [Āµmol/min/mg] Specific Activity Maximum [Āµmol/min/mg] Comment Organism
3.66
-
purified enzyme Trinickia caryophylli

Storage Stability

Storage Stability Organism
4Ā°C, 50% loss of activity within 1 month Trinickia caryophylli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-deoxy-L-galactose + NAD+ 73% of the activity compared to D-arabinose or L-xylose Trinickia caryophylli 6-deoxy-L-galactono-1,5-lactone + NADH
-
?
6-deoxy-L-galactose + NAD+ i.e. L-fucose Trinickia caryophylli 6-deoxy-L-galactono-1,5-lactone + NADH
-
?
D-arabinose + NAD+ 100% activity Trinickia caryophylli D-arabinono-1,4-lactone + NADH
-
?
D-ribose + NAD+ 8% of the activity compared to D-arabinose or L-xylose Trinickia caryophylli ? + NADH
-
?
D-xylose + NAD+ 9% of the activity compared to D-arabinose or L-xylose Trinickia caryophylli D-xylono-1,5-lactone + NADH + H+
-
?
L-glucose + NAD+ 68% of the activity compared to D-arabinose or L-xylose Trinickia caryophylli L-glucono-1,5-lactone + NADH
-
?
L-xylose + NAD+ 100% activity Trinickia caryophylli L-xylono-1,5-lactone + NADH
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
Trinickia caryophylli

Cofactor

Cofactor Comment Organism Structure
NAD+ also able to use NADP+ Trinickia caryophylli
NADP+ 10-20% of the activity compared to NAD+ Trinickia caryophylli