Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.103 extracted from

  • Wagner, M.; Andreesen, J.R.
    Purification and characterization of threonine dehydrogenase from Clostridium sticklandi (1995), Arch. Microbiol., 163, 286-290.
    View publication on PubMed
Search for more literature for 1.1.1.103

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 0.9 mM, 61% inhibiton Acetoanaerobium sticklandii
Cu2+ 0.9 mM, 54% inhibiton Acetoanaerobium sticklandii
additional information no inhibition by Ni2+, Ca2+, Mg2+, Mn2+ Acetoanaerobium sticklandii
Zn2+ 0.9 mM, 23% inhibiton Acetoanaerobium sticklandii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 NAD+ pH 9.0, 37°C Acetoanaerobium sticklandii
18
-
 L-threonine pH 9.0, 37°C Acetoanaerobium sticklandii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 gel filtration, monomeric enzyme Acetoanaerobium sticklandii
80000
-
 gel filtration, dimeric enzyme Acetoanaerobium sticklandii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-threonine + NAD+ Acetoanaerobium sticklandii
-
 (2S)-2-amino-3-oxobutanoate + NADH + H+
-
 ?
L-threonine + NAD+ Acetoanaerobium sticklandii DSM 519
-
 (2S)-2-amino-3-oxobutanoate + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Acetoanaerobium sticklandii E3PS87
-
-
Acetoanaerobium sticklandii DSM 519 E3PS87
-
-

Purification (Commentary)

Purification (Comment) Organism
chromatography on Q-Sepharose and Reactive Green 19-agarose Acetoanaerobium sticklandii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
234
-
 L-threonine, pH 9.0, 37°C Acetoanaerobium sticklandii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetoin + NAD+ 1% of the activity compared to L-threonine Acetoanaerobium sticklandii butan-2,3-dione + NADH + H+
-
 ?
acetoin + NAD+ 1% of the activity compared to L-threonine Acetoanaerobium sticklandii DSM 519 butan-2,3-dione + NADH + H+
-
 ?
DL-3-hydroxynorvaline + NAD+ 20% of the activity compared to L-threonine Acetoanaerobium sticklandii 3-oxonorvaline + NADH + H+
-
 ?
DL-3-hydroxynorvaline + NAD+ 20% of the activity compared to L-threonine Acetoanaerobium sticklandii DSM 519 3-oxonorvaline + NADH + H+
-
 ?
L-threonine + NAD+
-
 Acetoanaerobium sticklandii (2S)-2-amino-3-oxobutanoate + NADH + H+
-
 ?
L-threonine + NAD+
-
 Acetoanaerobium sticklandii DSM 519 (2S)-2-amino-3-oxobutanoate + NADH + H+
-
 ?
additional information no activity with L-theronine methyl ester, L-threonine amide, DL-threonine hydroxamate, L-homoserine, (R)-1-amino-2-propanol, L-malate, (R)-3-hydroxybutyrate, DL-threo-phenylserine, L-serine Acetoanaerobium sticklandii ?
-
 ?
additional information no activity with L-theronine methyl ester, L-threonine amide, DL-threonine hydroxamate, L-homoserine, (R)-1-amino-2-propanol, L-malate, (R)-3-hydroxybutyrate, DL-threo-phenylserine, L-serine Acetoanaerobium sticklandii DSM 519 ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 35000, SDS-PAGE, acitve in monomeric and in dimeric enzyme form Acetoanaerobium sticklandii

Synonyms

Synonyms Comment Organism
CLOST_1621 locus name Acetoanaerobium sticklandii
threonine dehydrogenase
-
 Acetoanaerobium sticklandii

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 8
-
 Acetoanaerobium sticklandii

Cofactor

Cofactor Comment Organism Structure
NAD+ NADP+, thio-NAD+, or 3-acetylpyridine adenine dinucleotide cannot substitute for NAD+ Acetoanaerobium sticklandii