Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | L-ThrDH is unaffected by EDTA, Li2SO4, MgCl2, MnCl2, CaCl2, NiCl2, CoCl2, BaCl2, HgCl2, CdSO4, CuSO4, ZnCl2, or iodoacetic acid, each at 1 mM | Thermoplasma volcanium |
Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Thermoplasma volcanium |
Crystallization (Comment) | Organism |
---|---|
purified wild-type enzyme or enzyme mutant Y137F in complex with NAD+ and L-3-hydroxynorvaline or pyruvate or L-threonine, sitting drop vapor diffusion method, mixing of 0.001 ml of 40 mg/ml wild-type or 25 mg/ml mutant enzyme, and 1 mM NAD+ mixed with 0.001 ml of 100 mM cacodylate buffer, pH 6.4, 50% v/v 2-methyl-2,4-pentandiol, and 5% w/v PEG 8000, 20°C, crystals of ligand-bound wild-type or mutant enzymes by soaking the crystals in reservoir solution containing 0.3 M pyruvate for 2 h, or 0.1ML-threonine for 8 h or 0.1MDL-3-hydroxynorvaline for 7 h, respectively, X-ray diffraction structure determination and analysis at 1.77-2.07 A resolution, molecular replacement | Thermoplasma volcanium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | L-ThrDH is unaffected by EDTA, Li2SO4, MgCl2, MnCl2, CaCl2, NiCl2, CoCl2, BaCl2, HgCl2, CdSO4, CuSO4, ZnCl2, or iodoacetic acid, each at 1 mM | Thermoplasma volcanium | |
pyruvate | 20% inhibition at 10 mM | Thermoplasma volcanium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.185 | - |
NAD+ | pH 10.0, 70°C | Thermoplasma volcanium | |
17.5 | - |
L-threonine | pH 10.0, 70°C | Thermoplasma volcanium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
2 * 35000, SDS-PAGE, 2 * 35843, sequence calculation | Thermoplasma volcanium |
35843 | - |
2 * 35000, SDS-PAGE, 2 * 35843, sequence calculation | Thermoplasma volcanium |
77000 | - |
gel filtration | Thermoplasma volcanium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-threonine + NAD+ | Thermoplasma volcanium | - |
(2S)-2-amino-3-oxobutanoate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoplasma volcanium | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-threonine + NAD+ = L-2-amino-3-oxobutanoate + NADH + H+ | reaction mechanism involving catalytic residues T112, Y137, and K141, overview | Thermoplasma volcanium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-threonine + NAD+ | - |
Thermoplasma volcanium | (2S)-2-amino-3-oxobutanoate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 35000, SDS-PAGE, 2 * 35843, sequence calculation | Thermoplasma volcanium |
Synonyms | Comment | Organism |
---|---|---|
L-ThrDH | - |
Thermoplasma volcanium |
L-threonine dehydrogenase | - |
Thermoplasma volcanium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Thermoplasma volcanium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
purified recombinant enzyme, 10 min, 90% activity remaining | Thermoplasma volcanium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
- |
Thermoplasma volcanium |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | 9.5 | purified recombinant enzyme, 20 min, 50°C, no loss in activity | Thermoplasma volcanium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Thermoplasma volcanium |
General Information | Comment | Organism |
---|---|---|
additional information | active site structure and subunit interaction, overview | Thermoplasma volcanium |