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Literature summary for 1.1.1.103 extracted from

  • Lee, J.H.; Sung, B.H.; Kim, M.S.; Blattner, F.R.; Yoon, B.H.; Kim, J.H.; Kim, S.C.
    Metabolic engineering of a reduced-genome strain of Escherichia coli for L-threonine production (2009), Microb. Cell Fact., 8, 02.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis over-expression of a feedback-resistant threonine operon thrA*BC, with deletion of the genes that encode threonine dehydrogenase tdh and threonine transporters tdcC and sstT, and introduction of a mutant threonine exporter rhtA23 in Escherichia coliMDS42. The resulting strain shows about 83% increase in L-threonine production when cells are grown by flask fermentation, compared to a wild-type Escherichia coli strain MG1655 engineered with the same threonine-specific modifications described above Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
lacks 14.3% of its chromosome
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Escherichia coli MDS42
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lacks 14.3% of its chromosome
-

General Information

General Information Comment Organism
physiological function over-expression of a feedback-resistant threonine operon thrA*BC, with deletion of the genes that encode threonine dehydrogenase tdh and threonine transporters tdcC and sstT, and introduction of a mutant threonine exporter rhtA23 in Escherichia coliMDS42. The resulting strain shows about 83% increase in L-threonine production when cells are grown by flask fermentation, compared to a wild-type Escherichia coli strain MG1655 engineered with the same threonine-specific modifications described above Escherichia coli