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Literature summary for 1.1.1.103 extracted from

  • Boylan, S.A.; Dekker, E.E.
    L-threonine dehydrogenase (1981), J. Biol. Chem., 256, 1809-1815.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis-(2-nitrobenzoic acid) 0.25 mM, 90% inhibition, 67% activity is recovered after incubation with 1 mM, 2-mercaptoethanol or dithieothreitol for 15 min Escherichia coli K-12
Cu2+ 3.2 mM, 67% inhibition Escherichia coli K-12
N-ethylmaleimide 0.32 mM, 10 min, 37°C, 23% inhibition Escherichia coli K-12
Ni2+ 3.2 mM, 69% inhibition Escherichia coli K-12
p-mercuribenzoate 0.0013 mM, 75% inhibition, completely reversed within 20 min by addition of 0.02-0.2 mM 2-mercaptoethanol or dithiothreitol Escherichia coli K-12

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.19
-
NAD+
-
Escherichia coli K-12
1.43
-
L-threonine
-
Escherichia coli K-12

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 0.064 mM, 1.6fold activation, 3.2 mM, 2.4fold activation Escherichia coli K-12

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
140000
-
sucrose density and sedimentation equilibrium Escherichia coli K-12

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-threonine + NAD+ Escherichia coli K-12
-
(2S)-2-amino-3-oxobutanoate + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli K-12
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli K-12

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
34
-
-
Escherichia coli K-12

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-allothreonine + NAD+
-
Escherichia coli K-12 D-2-amino-3-oxobutanoate + NADH
-
?
DL-2-amino-3-hydroxypentanoate + NAD+
-
Escherichia coli K-12 L-2-amino-3-oxopentanoate + NADH
-
?
DL-2-amino-3-hydroxyvalerate + NAD+
-
Escherichia coli K-12 DL-2-amino-3-oxovalerate + NADH
-
?
DL-allothreonine + NAD+
-
Escherichia coli K-12 D-2-amino-3-oxobutanoate + NADH
-
?
DL-threo-beta-phenylserine + NAD+
-
Escherichia coli K-12 DL-2-amino-3-phenyl-3-oxopropionate + NADH
-
?
L-serine + NAD+
-
Escherichia coli K-12 L-2-amino-3-oxopropionate + NADH + H+
-
?
L-threonine + NAD+
-
Escherichia coli K-12 (2S)-2-amino-3-oxobutanoate + NADH + H+
-
?
L-threonine amide + NAD+
-
Escherichia coli K-12 L-2-amino-3-oxobutyramide + NADH
-
?
L-threonine methyl ester + NAD+
-
Escherichia coli K-12 L-2-amino-3-oxobutanoate methyl ester + NADH
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
135
-
L-threonine
-
Escherichia coli K-12

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.3
-
35% activity at pH 9.0, 6% activity at pH 11.0 Escherichia coli K-12

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Escherichia coli K-12