Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis-(2-nitrobenzoic acid) | 0.25 mM, 90% inhibition, 67% activity is recovered after incubation with 1 mM, 2-mercaptoethanol or dithieothreitol for 15 min | Escherichia coli K-12 | |
Cu2+ | 3.2 mM, 67% inhibition | Escherichia coli K-12 | |
N-ethylmaleimide | 0.32 mM, 10 min, 37°C, 23% inhibition | Escherichia coli K-12 | |
Ni2+ | 3.2 mM, 69% inhibition | Escherichia coli K-12 | |
p-mercuribenzoate | 0.0013 mM, 75% inhibition, completely reversed within 20 min by addition of 0.02-0.2 mM 2-mercaptoethanol or dithiothreitol | Escherichia coli K-12 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
NAD+ | - |
Escherichia coli K-12 | |
1.43 | - |
L-threonine | - |
Escherichia coli K-12 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | 0.064 mM, 1.6fold activation, 3.2 mM, 2.4fold activation | Escherichia coli K-12 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
140000 | - |
sucrose density and sedimentation equilibrium | Escherichia coli K-12 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-threonine + NAD+ | Escherichia coli K-12 | - |
(2S)-2-amino-3-oxobutanoate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli K-12 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli K-12 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
34 | - |
- |
Escherichia coli K-12 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-allothreonine + NAD+ | - |
Escherichia coli K-12 | D-2-amino-3-oxobutanoate + NADH | - |
? | |
DL-2-amino-3-hydroxypentanoate + NAD+ | - |
Escherichia coli K-12 | L-2-amino-3-oxopentanoate + NADH | - |
? | |
DL-2-amino-3-hydroxyvalerate + NAD+ | - |
Escherichia coli K-12 | DL-2-amino-3-oxovalerate + NADH | - |
? | |
DL-allothreonine + NAD+ | - |
Escherichia coli K-12 | D-2-amino-3-oxobutanoate + NADH | - |
? | |
DL-threo-beta-phenylserine + NAD+ | - |
Escherichia coli K-12 | DL-2-amino-3-phenyl-3-oxopropionate + NADH | - |
? | |
L-serine + NAD+ | - |
Escherichia coli K-12 | L-2-amino-3-oxopropionate + NADH + H+ | - |
? | |
L-threonine + NAD+ | - |
Escherichia coli K-12 | (2S)-2-amino-3-oxobutanoate + NADH + H+ | - |
? | |
L-threonine amide + NAD+ | - |
Escherichia coli K-12 | L-2-amino-3-oxobutyramide + NADH | - |
? | |
L-threonine methyl ester + NAD+ | - |
Escherichia coli K-12 | L-2-amino-3-oxobutanoate methyl ester + NADH | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
135 | - |
L-threonine | - |
Escherichia coli K-12 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10.3 | - |
35% activity at pH 9.0, 6% activity at pH 11.0 | Escherichia coli K-12 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Escherichia coli K-12 |