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Literature summary for 1.1.1.100 extracted from

  • Sun, Y.H.; Cheng, Q.; Tian, W.X.; Wu, X.D.
    A substitutive substrate for measurements of beta-ketoacyl reductases in two fatty acid synthase systems (2008), J. Biochem. Biophys. Methods, 70, 850-856.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
NH2-terminally His-tagged FabG expressed in Escherichia coli strain BL21(DE3) Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
epigallocatechin gallate
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
NADPH
-
Escherichia coli
0.127
-
ethyl acetoacetate
-
Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by nickel affinity chromatography Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, phosphate buffer, 10% glycerol Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ethyl acetoacetate + NADPH
-
Escherichia coli ethyl 3-hydroxybutyrate + NADP+
-
?
methyl acetoacetate + NADPH is not a good substrate as ethyl acetoacetate Escherichia coli ? + NADP+
-
?
propyl acetoacetate + NADPH as substrate it is limited to its weak solubility Escherichia coli ? + NADP+
-
?

Synonyms

Synonyms Comment Organism
beta-ketoacyl-ACP reductase
-
Escherichia coli
FabG
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.3
-
ethyl acetoacetate
-
Escherichia coli
0.59
-
NADPH
-
Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Escherichia coli

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00554
-
at pH 8.0 Escherichia coli epigallocatechin gallate
0.0084
-
at pH 8.3 Escherichia coli epigallocatechin gallate
0.0105
-
at pH 7.5 Escherichia coli epigallocatechin gallate
0.0336
-
at pH 8.5 Escherichia coli epigallocatechin gallate
0.0578
-
at pH 7.0 Escherichia coli epigallocatechin gallate
0.12
-
at pH 9.0 Escherichia coli epigallocatechin gallate
0.1223
-
at pH 6.5 Escherichia coli epigallocatechin gallate