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Literature summary for 1.1.1.100 extracted from

  • Kristan, K.; Bratkovic, T.; Sova, M.; Gobec, S.; Prezelj, A.; Urleb, U.
    Novel inhibitors of beta-ketoacyl-ACP reductase from Escherichia coli (2009), Chem. Biol. Interact., 178, 310-316.
    View publication on PubMed
Search for more literature for 1.1.1.100

Cloned(Commentary)

Cloned (Comment) Organism
pET15b-fabG vector construct transformed into the Escherichia coli BL21 DE3 strain Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
(E)-1-(4-methylpiperidin-1-yl)-3-phenylprop-2-en-1-one 80% residual activity Escherichia coli
(E)-2-nitrophenyl cinnamate
-
 Escherichia coli
(E)-3-phenoxybenzyl 3-(benzo[d][1,3]dioxol-5-yl)acrylate 61% residual activity Escherichia coli
(E)-4-cyanophenyl 3-(benzo[d][1,3]dioxol-5-yl)acrylate
-
 Escherichia coli
(E)-benzyl 3-(benzo[d][1,3]dioxol-5-yl)acrylate 71% residual activity Escherichia coli
(E)-phenyl 3-(benzo[d][1,3]dioxol-5-yl)acrylate 69% residual activity Escherichia coli
additional information cinnamic acid derivatives can be accommodated in the substrate-binding region of the active site, above the nicotinamide moiety of the NADPH cofactor Escherichia coli
N-(1,3-dioxoisoindolin-2-yl)-2-oxo-2H-chromene-3-carboxamide 69% residual activity Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0AEK2
-
-

Purification (Commentary)

Purification (Comment) Organism
by sonication, centrifugation and on Ni-NTA column Escherichia coli

Storage Stability

Storage Stability Organism
-80°C, 10% glycerol Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetoacetyl-CoA + NADPH + H+
-
 Escherichia coli D-beta-hydroxybutyryl-CoA + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
beta-ketoacyl-ACP reductase
-
 Escherichia coli
beta-ketoacyl-[acyl carrier protein] reductase
-
 Escherichia coli
FabG
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Escherichia coli

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.18
-
 pH 7.0 Escherichia coli (E)-phenyl 3-(benzo[d][1,3]dioxol-5-yl)acrylate
0.18
-
 pH 7.0 Escherichia coli (E)-4-cyanophenyl 3-(benzo[d][1,3]dioxol-5-yl)acrylate
0.215
-
 pH 7.0 Escherichia coli N-(1,3-dioxoisoindolin-2-yl)-2-oxo-2H-chromene-3-carboxamide
0.45
-
 pH 7.0 Escherichia coli (E)-2-nitrophenyl cinnamate