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Literature summary for 1.1.1.100 extracted from

  • Hoelsch, K.; Havel, J.; Haslbeck, M.; Weuster-Botz, D.
    Identification, cloning, and characterization of a novel ketoreductase from the cyanobacterium Synechococcus sp. strain PCC 7942 (2008), Appl. Environ. Microbiol., 74, 6697-6702.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.100

Activating Compound

Activating Compound Comment Organism Structure
additional information variation of the buffer molarity between 25 mM and 200 mM or addition of NaCl up to 500 mM has no significant effect on enzyme activity Synechococcus elongatus PCC 7942 = FACHB-805

Cloned(Commentary)

Cloned (Comment) Organism
transformation of the fabG gene encoding 3-ketoacyl-[acyl-carrier-protein] reductase in fusion with a His6-MBP-tag (ligated into vector pETM-41) into Escherichia coli XL-10 Gold. After isolation from XL-10 cells and DNA sequencing, transformed into Escherichia coli Tuner (DE3) cells Synechococcus elongatus PCC 7942 = FACHB-805

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.92
-
 ethylbenzoylacetate with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 Synechococcus elongatus PCC 7942 = FACHB-805
2.71
-
 alpha-chloroacetophenone with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 Synechococcus elongatus PCC 7942 = FACHB-805
5.72
-
 2',3',4',5',6'-pentafluoroacetophenone with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 Synechococcus elongatus PCC 7942 = FACHB-805
6.03
-
 propiophenone with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 Synechococcus elongatus PCC 7942 = FACHB-805
7.38
-
 3'-fluoroacetophenone with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 Synechococcus elongatus PCC 7942 = FACHB-805
8.31
-
 ethyl 4-chloroacetoacetate with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 Synechococcus elongatus PCC 7942 = FACHB-805
12.29
-
 4'-fluoroacetophenone with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 Synechococcus elongatus PCC 7942 = FACHB-805
14.34
-
 4'-chloroacetophenone with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 Synechococcus elongatus PCC 7942 = FACHB-805
46.92
-
 acetophenone with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 Synechococcus elongatus PCC 7942 = FACHB-805
89.2
-
 1,1,1-trifluoroacetone with 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 Synechococcus elongatus PCC 7942 = FACHB-805

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25500
-
 4 * 25500, SDS-PAGE Synechococcus elongatus PCC 7942 = FACHB-805
102000
-
 gel filtration Synechococcus elongatus PCC 7942 = FACHB-805

Organism

Organism UniProt Comment Textmining
Synechococcus elongatus PCC 7942 = FACHB-805 Q31QF3
-
-

Purification (Commentary)

Purification (Comment) Organism
by gel filtration and ion exchange chromatography, to homogeneity Synechococcus elongatus PCC 7942 = FACHB-805

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.02
-
 with 2-octanone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 Synechococcus elongatus PCC 7942 = FACHB-805
0.08
-
 with 4'-fluoroacetophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 Synechococcus elongatus PCC 7942 = FACHB-805
0.13
-
 with 3'-fluoroacetophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 Synechococcus elongatus PCC 7942 = FACHB-805
0.13
-
 with acetophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 Synechococcus elongatus PCC 7942 = FACHB-805
0.15
-
 with 4'-chloroacetophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 Synechococcus elongatus PCC 7942 = FACHB-805
0.16
-
 with propiophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 Synechococcus elongatus PCC 7942 = FACHB-805
1.03
-
 with 1,1,1-trifluoroacetone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 Synechococcus elongatus PCC 7942 = FACHB-805
1.16
-
 with ethylbenzoylacetate as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 Synechococcus elongatus PCC 7942 = FACHB-805
2.45
-
 with alpha-chloroacetophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 Synechococcus elongatus PCC 7942 = FACHB-805
3.93
-
 with 2',3',4',5',6'-pentafluoroacetophenone as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 Synechococcus elongatus PCC 7942 = FACHB-805
38.29
-
 with ethyl 4-chloroacetoacetate as substrate, 4 mM NADPH, in 0.1 M sodium phosphate buffer, pH 7.0 Synechococcus elongatus PCC 7942 = FACHB-805

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,1,1-trifluoroacetone + NADPH 792% relative activity compared to acetophenone Synechococcus elongatus PCC 7942 = FACHB-805 ? + NADP+
-
 ?
2',3',4',5',6'-pentafluoroacetophenone + NADPH 3023% relative activity compared to acetophenone Synechococcus elongatus PCC 7942 = FACHB-805 ? + NADP+
-
 ?
2-octanone + NADPH 15% relative activity compared to acetophenone Synechococcus elongatus PCC 7942 = FACHB-805 ? + NADP+
-
 ?
3'-fluoroacetophenone + NADPH 100% relative activity compared to acetophenone Synechococcus elongatus PCC 7942 = FACHB-805 ? + NADP+
-
 ?
4'-chloroacetophenone + NADPH 115% relative activity compared to acetophenone Synechococcus elongatus PCC 7942 = FACHB-805 ? + NADP+
-
 ?
4'-fluoroacetophenone + NADPH 62% relative activity compared to acetophenone Synechococcus elongatus PCC 7942 = FACHB-805 ? + NADP+
-
 ?
acetophenone + NADPH 100% relative activity Synechococcus elongatus PCC 7942 = FACHB-805 ? + NADP+
-
 ?
alpha-chloroacetophenone + NADPH 1885% relative activity compared to acetophenone Synechococcus elongatus PCC 7942 = FACHB-805 ? + NADP+
-
 ?
ethyl 4-chloroacetoacetate + NADPH 29454% relative activity compared to acetophenone Synechococcus elongatus PCC 7942 = FACHB-805 ? + NADP+
-
 ?
ethylbenzoylacetate + NADPH 892% relative activity compared to acetophenone Synechococcus elongatus PCC 7942 = FACHB-805 ? + NADP+
-
 ?
propiophenone + NADPH 123% relative activity compared to acetophenone Synechococcus elongatus PCC 7942 = FACHB-805 ? + NADP+
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 25500, SDS-PAGE Synechococcus elongatus PCC 7942 = FACHB-805

Synonyms

Synonyms Comment Organism
3-ketoacyl-[acyl-carrier-protein] reductase
-
 Synechococcus elongatus PCC 7942 = FACHB-805

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
44
-
-
 Synechococcus elongatus PCC 7942 = FACHB-805

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 55 30°C: about 55% of maximal activity, 55°C: about 65% of maximal activity Synechococcus elongatus PCC 7942 = FACHB-805

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Synechococcus elongatus PCC 7942 = FACHB-805

pH Range

pH Minimum pH Maximum Comment Organism
4 10 pH 4: about 60% of maximal activity, pH 10: about 75% of maximal activity Synechococcus elongatus PCC 7942 = FACHB-805