Application | Comment | Organism |
---|---|---|
drug development | the enzyme has no isoforms and thus is a good target for inhibitor design | Plasmodium falciparum |
Cloned (Comment) | Organism |
---|---|
gene fabG, DNA and amino acid sequence determination and analysis, expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | cooperativity kinetics, binding constants of substrates and cofactors | Plasmodium falciparum | |
0.043 | - |
NADPH | pH 6.8, 22°C, recombinant enzyme | Plasmodium falciparum | |
0.43 | - |
acetoacetyl-CoA | pH 6.8, 22°C, recombinant enzyme | Plasmodium falciparum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-oxoacyl-[acyl-carrier protein] + NADPH | Plasmodium falciparum | - |
3-hydroxyacyl-[acyl-carrier protein] + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
gene fabG, no isozymes | - |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Plasmodium falciparum |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ = a 3-oxoacyl-[acyl-carrier protein] + NADPH + H+ | cofactor and substrate binding kinetics, allosteric regulation mechanism, overview | Plasmodium falciparum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
59.8 | - |
purified recombinant enzyme | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-oxoacyl-[acyl-carrier protein] + NADPH | - |
Plasmodium falciparum | 3-hydroxyacyl-[acyl-carrier protein] + NADP+ | - |
r | |
3-oxoacyl-[acyl-carrier protein] + NADPH | cooperative transitions in the enzyme due to cofactor and [acyl-carrier-protein] binding | Plasmodium falciparum | 3-hydroxyacyl-[acyl-carrier protein] + NADP+ | - |
r | |
acetoacetyl-CoA + NADPH | - |
Plasmodium falciparum | 3-hydroxybutyryl-CoA + NADP+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
beta-ketoacyl acyl carrier protein reductase | - |
Plasmodium falciparum |
FabG | - |
Plasmodium falciparum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Plasmodium falciparum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
259 | - |
NADPH | pH 6.8, 22°C, recombinant enzyme | Plasmodium falciparum | |
259 | - |
acetoacetyl-CoA | pH 6.8, 22°C, recombinant enzyme | Plasmodium falciparum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at | Plasmodium falciparum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | cooperative transitions in the enzyme due to cofactor and [acyl-carrier-protein] binding, cooperative cofactor binding | Plasmodium falciparum | |
NADPH | cooperative transitions in the enzyme due to cofactor and [acyl-carrier-protein] binding, cooperative allosteric cofactor binding in presence of [acyl-carrier-protein] | Plasmodium falciparum |