Literature summary for 1.1.1.100 extracted from

Patel, M.P.; Liu, W.S.; West, J.; Tew, D.; Meek, T.D.; Thrall, S.H.
Kinetic and chemical mechanisms of the fabG-encoded Streptococcus pneumoniae beta-ketoacyl-ACP reductase (2005), Biochemistry, 44, 16753-16765.

Search for more literature for 1.1.1.100

Inhibitors

Inhibitors	Comment	Organism	Structure
DL-3-hydroxybutyryl-CoA	competitive product inhibition	Streptococcus pneumoniae
NADP+	competitive product inhibition	Streptococcus pneumoniae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	detailed steady-state kinetic analysis, solvent and multiple kinetic isotope effects, random bi bi kinetic mechanism	Streptococcus pneumoniae
0.4	-	NADPH	pH 7.6, 30°C	Streptococcus pneumoniae
2.2	-	acetoacetyl-CoA	pH 7.6, 30°C	Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
acetoacetyl-CoA + NADPH	Streptococcus pneumoniae	-	3-hydroxybutyryl-CoA + NADP+	-	?

Organism

Organism	UniProt	Comment	Textmining
Streptococcus pneumoniae	-	gene fabG	-

Reaction

Reaction	Comment	Organism	Reaction ID
a (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ = a 3-oxoacyl-[acyl-carrier protein] + NADPH + H+	reaction mechanism, random bi bi kinetic mechanism	Streptococcus pneumoniae	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acetoacetyl-CoA + NADPH	-	Streptococcus pneumoniae	3-hydroxybutyryl-CoA + NADP+	-	?

Synonyms

Synonyms	Comment	Organism
beta-ketoacyl-ACP reductase	-	Streptococcus pneumoniae
KACPR	-	Streptococcus pneumoniae

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Streptococcus pneumoniae

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
11	-	NADPH	pH 7.6, 30°C	Streptococcus pneumoniae
11	-	acetoacetyl-CoA	pH 7.6, 30°C	Streptococcus pneumoniae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.6	-	-	Streptococcus pneumoniae

pH Range

pH Minimum	pH Maximum	Comment	Organism
5.5	9.5	pH profile, detailed mechanistic pH-dependence analysis, overview	Streptococcus pneumoniae

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	-	Streptococcus pneumoniae

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Release 2023.1 (January 2023)