Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
18 mg/ml purified enzyme complexed with NADPH, in 10 mM Tris-HCl, pH 7.5, 2 mM 2-mercaptoethanol, 20% glycerol, replacement buffer is 10 mM Tris-HCl, pH 7.5, 2 mM 2-mercaptoethanol, mixed with 12.9 mM NADPH, in a molar ratio of enzyme and cofactor of 1:8, equal volume of 0.003 ml of enzyme complex mixture and well solution, containing 15% PEG 8000, 50 mM potassium phosphate, and 0.1 M MES, pH 6.5, 1 week, X-ray diffraction structure determination and analysis, molecular replacement method, 1.96 A resolution, molecular modeling | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
N107D | site-directed mutagenesis, active site residue mutant, inactive | Homo sapiens |
N107L | site-directed mutagenesis, active site residue mutant, inactive | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
NADPH | pH 7.0, 25°C, wild-type enzyme | Homo sapiens | |
0.077 | - |
diacetyl | pH 7.0, 25°C, wild-type enzyme | Homo sapiens | |
26 | - |
diacetyl | pH 7.0, 25°C, mutant N107D | Homo sapiens | |
31 | - |
diacetyl | pH 7.0, 25°C, mutant N107L | Homo sapiens | |
95 | - |
NADPH | pH 7.0, 25°C, mutant N107D | Homo sapiens | |
97 | - |
NADPH | pH 7.0, 25°C, mutant N107L | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-xylulose + NADPH + H+ | Homo sapiens | the enzyme is involved in the uronate cycle of glucose metabolism | L-xylitol + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q7Z4W1 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
xylitol + NADP+ = L-xylulose + NADPH + H+ | active site residues are Cys138, Val143, His146, Trp191, and Met200, the catalytic tetrad is formed by Asn107, Ser136, Tyr149, and Lys153, substrate binding site structure, enzyme with dual function showing L-xylulose reductase activity and dicarbonyl reductase activity, EC 1.1.1.5 | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diacetyl + NAD(P)H | dicarbonyl reductase activity | Homo sapiens | acetoin + NAD(P)+ | - |
r | |
L-xylulose + NADPH + H+ | the enzyme is involved in the uronate cycle of glucose metabolism | Homo sapiens | L-xylitol + NADP+ | - |
r | |
L-xylulose + NADPH + H+ | L-xylulose reductase activity | Homo sapiens | L-xylitol + NADP+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
More | enzyme belongs to the short-chain dehydrogenase/reductase family | Homo sapiens |
XR | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0008 | - |
diacetyl | pH 7.0, 25°C, mutant N107L | Homo sapiens | |
0.009 | - |
diacetyl | pH 7.0, 25°C, mutant N107D | Homo sapiens | |
1.57 | - |
diacetyl | pH 7.0, 25°C, wild-type enzyme | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | binding structure determination | Homo sapiens | |
NADPH | binding structure determination | Homo sapiens |