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Literature summary for 1.1.1.10 extracted from

  • Doten, R.C.; Mortlock, R.P.
    Characterization of xylitol-utilizing mutants of Erwinia uredovora (1985), J. Bacteriol., 161, 529-533.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
Tn5 transposon transfer Pantoea ananatis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
48
-
xylitol
-
Pantoea ananatis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
136000
-
non-denaturing PAGE Pantoea ananatis

Organism

Organism UniProt Comment Textmining
Pantoea ananatis
-
xylitol utilizing mutant strains, e.g. DM101, of strain 19321
-

Purification (Commentary)

Purification (Comment) Organism
partial from strain DM101 Pantoea ananatis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.23 0.32 xylitol dehydrogenase activity Pantoea ananatis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-threitol + NAD+
-
Pantoea ananatis D-threose + NADH
-
r
additional information minimal oxidative activity with D-arabitol, mannitol, 1,2-propanediol and 2,3-butanediol Pantoea ananatis ?
-
?
xylitol + NADP+
-
Pantoea ananatis L-xylulose + NADPH + H+ strain DM101 and DM122, product identification is not exact, could also be D-xylulose ?

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Pantoea ananatis