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ATP phosphohydrolase (DNA helix unwinding)
DNA helicases utilize the energy from ATP hydrolysis to unwind double-stranded DNA. Some of them unwind duplex DNA with a 3' to 5' polarity [1,3,5,8], others show 5' to 3' polarity [10,11,12,13] or unwind DNA in both directions [14,15]. Some helicases unwind DNA as well as RNA [9,10]. May be identical with EC 184.108.40.206 (RNA helicase).
helicase-catalyzed DNA unwinding by nonstructural protein 3 analyzed by molecular beacon-based helicase assay (MBHA), NTP binding occurs with similar affinities, dNTPs support faster DNA unwinding, dTTP supporting faster rates than any other canonical dNTP