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EC Tree
IUBMB Comments The enzyme has been originally characterized from the protist Euglena gracilis [1,2]. The enzyme from the archaeon Sulfolobus solfataricus can transfer the propylamine moiety from S-adenosyl 3-(methylsulfanyl)propylamine to putrescine, sym-norspermidine and spermidine with lower efficiency . cf. EC 2.5.1.16 (spermidine synthase) and EC 2.5.1.22 (spermine synthase).
The expected taxonomic range for this enzyme is: Eukaryota, Archaea
Synonyms
HbSpe 1, propylaminc transferase,
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propylaminc transferase
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HbSpe 1
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S-adenosyl 3-(methylsulfanyl)propylamine + propane-1,3-diamine = S-methyl-5'-thioadenosine + bis(3-aminopropyl)amine
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aminopropyl group transfer
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S-adenosyl 3-(methylthio)propylamine:propane-1,3-diamine 3-aminopropyltransferase
The enzyme has been originally characterized from the protist Euglena gracilis [1,2]. The enzyme from the archaeon Sulfolobus solfataricus can transfer the propylamine moiety from S-adenosyl 3-(methylsulfanyl)propylamine to putrescine, sym-norspermidine and spermidine with lower efficiency [3]. cf. EC 2.5.1.16 (spermidine synthase) and EC 2.5.1.22 (spermine synthase).
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S-adenosyl 3-(methylthio)propylamine + propane-1,3-diamine
S-methyl-5'-thioadenosine + bis(3-aminopropyl)amine
S-adenosyl 3-(methylthio)propylamine + spermidine
S-methyl-5'-thioadenosine + thermospermine + H+
S-adenosylmethioninamine + propane-1,3-diamine
5'-methylthioadenosine + N-(3-aminopropyl)-1,3-diaminopropane
S-adenosylmethioninamine + propane-1,3-diamine
5'-S-methyl-5'-thioadenosine + bis(3-aminopropyl)amine
S-adenosylmethioninamine + putrescine
5'-S-methyl-5'-thioadenosine + spermidine
S-adenosylmethioninamine + spermidine
5'-S-methyl-5'-thioadenosine + spermine
S-adenosylmethioninamine + sym-norspermidine
5'-S-methyl-5'-thioadenosine + ?
S-adenosyl 3-(methylthio)propylamine + propane-1,3-diamine
S-methyl-5'-thioadenosine + bis(3-aminopropyl)amine
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?
S-adenosyl 3-(methylthio)propylamine + propane-1,3-diamine
S-methyl-5'-thioadenosine + bis(3-aminopropyl)amine
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?
S-adenosyl 3-(methylthio)propylamine + spermidine
S-methyl-5'-thioadenosine + thermospermine + H+
at 5% of the specific activity compared to propane-1,3-diamine
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?
S-adenosyl 3-(methylthio)propylamine + spermidine
S-methyl-5'-thioadenosine + thermospermine + H+
at 5% of the specific activity compared to propane-1,3-diamine
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?
S-adenosylmethioninamine + propane-1,3-diamine
5'-methylthioadenosine + N-(3-aminopropyl)-1,3-diaminopropane
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?
S-adenosylmethioninamine + propane-1,3-diamine
5'-methylthioadenosine + N-(3-aminopropyl)-1,3-diaminopropane
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i.e. decarboxylated S-adenosylmethionine + 1,3-diaminopropane
i.e. sym-norspermidine
?
S-adenosylmethioninamine + propane-1,3-diamine
5'-S-methyl-5'-thioadenosine + bis(3-aminopropyl)amine
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ir
S-adenosylmethioninamine + propane-1,3-diamine
5'-S-methyl-5'-thioadenosine + bis(3-aminopropyl)amine
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ir
S-adenosylmethioninamine + putrescine
5'-S-methyl-5'-thioadenosine + spermidine
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36.7% of the activity compared to activity with 1,3-diaminopropane, propylamine transfer is operated through a sequential mechanism
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ir
S-adenosylmethioninamine + putrescine
5'-S-methyl-5'-thioadenosine + spermidine
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36.7% of the activity compared to activity with 1,3-diaminopropane, propylamine transfer is operated through a sequential mechanism
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ir
S-adenosylmethioninamine + spermidine
5'-S-methyl-5'-thioadenosine + spermine
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6.8% of the activity compared to activity with 1,3-diaminopropane, propylamine transfer is operated through a sequential mechanism
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ir
S-adenosylmethioninamine + spermidine
5'-S-methyl-5'-thioadenosine + spermine
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6.8% of the activity compared to activity with 1,3-diaminopropane, propylamine transfer is operated through a sequential mechanism
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ir
S-adenosylmethioninamine + sym-norspermidine
5'-S-methyl-5'-thioadenosine + ?
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17.5% of the activity compared to activity with 1,3-diaminopropane, propylamine transfer is operated through a sequential mechanism
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ir
S-adenosylmethioninamine + sym-norspermidine
5'-S-methyl-5'-thioadenosine + ?
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17.5% of the activity compared to activity with 1,3-diaminopropane, propylamine transfer is operated through a sequential mechanism
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ir
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S-adenosylmethioninamine + propane-1,3-diamine
5'-methylthioadenosine + N-(3-aminopropyl)-1,3-diaminopropane
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?
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5'-isobutylthioadenosine
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5'-methylthiotubercidin
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5'-S-ethyl-5'-thioadenosine
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5'-S-methyl-5'-thioadenosine
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powerful competitive inhibitor
n-propylthioadenosine
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S-adenosyl(5')-3-thiopropylamine
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S-adenosyl-3-thio-1,8-diaminooctane
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S-adenosyl-3-thiopropylamine
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1.675
propane-1,3-diamine
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pH 7.5, 78°C
3.85
putrescine
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pH 7.5, 78°C
0.0079
S-adenosylmethioninamine
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pH 7.5, 78°C
1.539
spermidine
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pH 7.5, 78°C
0.954
sym-norspermidine
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pH 7.5, 78°C
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0.0037
5'-S-methyl-5'-thioadenosine
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pH 7.5, 78°C
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0.15
5'-isobutylthioadenosine
Saccharolobus solfataricus
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pH 7.5, 78°C
0.1
5'-S-ethyl-5'-thioadenosine
Saccharolobus solfataricus
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pH 7.5, 78°C
0.014
5'-S-methyl-5'-thioadenosine
Saccharolobus solfataricus
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pH 7.5, 78°C
0.1
A9154C
Saccharolobus solfataricus
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pH 7.5, 78°C
0.12
n-propylthioadenosine
Saccharolobus solfataricus
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pH 7.5, 78°C
0.021
S-adenosyl-3-thio-1,8-diaminooctane
Saccharolobus solfataricus
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pH 7.5, 78°C
0.016
S-adenosyl-3-thiopropylamine
Saccharolobus solfataricus
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pH 7.5, 78°C
0.1
sinefungin
Saccharolobus solfataricus
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pH 7.5, 78°C
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0.002
pH 7.5, 95°C, substrate: spermidine
0.038
pH 7.5, 95°C, substrate: propane-1,3-diamine
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7.5
assay at
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6 - 9.5
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pH 6: about 50% of maximal activity, pH 9.5: about 50% of maximal activity
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50 - 110
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50°C: about 40% of maximal activity, 110°C: about 45% of maximal activity, 120°C: about 20% of maximal activity
60 - 120
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less than 5% of maximal activity up to 45°C. 60°C: about 15% of maximal activity, 75°C: about 50% of maximal activity, 120°C: about 50% of maximal activity
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5.3
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isoelectric focusing
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Z, strain 1224-5/25
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UniProt
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UniProt
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brenda
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brenda
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no activity is associated with the cellular particulate fraction
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no activity is associated with the cellular particulate fraction
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SPEE2_HYPBU
Hyperthermus butylicus (strain DSM 5456 / JCM 9403 / PLM1-5)
305
0
34437
Swiss-Prot
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SPEE_SACS2
Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
301
0
34854
Swiss-Prot
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SPEE_PYRFU
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
281
0
32334
Swiss-Prot
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A0A5E4HM87_9ARCH
618
6
68208
TrEMBL
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A0A5E4HPL9_9ARCH
497
7
54234
TrEMBL
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A0A5E4HFN2_9ARCH
505
7
54975
TrEMBL
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112000
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ultracentrifugation
34437
x * 34437, calculation from sequence
35000
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3 * 35000, SDS-PAGE
37000
x * 37000, SDS-PAGE
110000
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gel filtration
110000
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gel filtration, ultracentrifugation analysis
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?
x * 37000, SDS-PAGE
?
x * 34437, calculation from sequence
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x * 37000, SDS-PAGE
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?
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x * 34437, calculation from sequence
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trimer
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trimer
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3 * 35000, SDS-PAGE
trimer
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3 * 35000, SDS-PAGE
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100
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1 h, completely stable
120
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no protection by substrate against thermal inactivation at 120°C is observed by incubating the enzyme in the presence of polyamines
25 - 85
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protein retains its quarternary structure between 25°C and 28°C. A highly reversible subtle conformational transition is detected by numerous structure-dependent techniques over 40-45°C, with a midpoint centered at 45°C
87
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the mechanism by which the protein achieves thermal stabilization is driven by a conformational equilibrium between two forms of different stability. The stability of each form towards denaturation is characterized by a specific temperature dependence. The low temperature form (form A), is stable over 12-89°C. Its stability maximum is 36.8 kJ/mol at 50°C. Form B, which is populated at higher temperature, spans the interval 28-146°C. Its stability maximum is 71.6 kJ/mol at 87°C
90
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rapidly looses activity above 90°C and can no longer be considered native
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-20°C, pH 7.5, stable for 1 months
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expression in Escherichia coli with an N-terminal 6* His sequence
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no difference in its activity between the cells harvested during the logarithmic or the stationary phase of growth. Propylamine transferase is not induced by the addition of either 1 mM 1,3-diaminopropane or sym-norspermidine or spermidine to the standard culture medium
no difference in its activity between the cells harvested during the logarithmic or the stationary phase of growth. Propylamine transferase is not induced by the addition of either 1 mM 1,3-diaminopropane or sym-norspermidine or spermidine to the standard culture medium
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no difference in its activity between the cells harvested during the logarithmic or the stationary phase of growth. Propylamine transferase is not induced by the addition of either 1 mM 1,3-diaminopropane or sym-norspermidine or spermidine to the standard culture medium
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Aleksijevic, A.; Grove, J.; Schuber, F.
Studies on polyamine biosynthesis in Euglena gracilis
Biochim. Biophys. Acta
565
199-207
1979
Euglena gracilis
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Cacciapuoti, G.; Porcelli, M.; Carteni-Farina, M.; Gambacorta, A.; Zappia, V.
Purification and characterization of propylamine transferase from Sulfolobus solfataricus, an extreme thermophilic archaebacterium
Eur. J. Biochem.
161
263-271
1986
Saccharolobus solfataricus, Saccharolobus solfataricus MT-4 / DSM 5833
brenda
Facchiano, F.; Ragone, R.; Porcelli, M.; Cacciapuoti, G.; Colonna, G.
Effect of temperature on the propylamine transferase from Sulfolobus solfataricus, an extreme thermophilic archaebacterium. 1. Conformational behavior of the oligomeric enzyme in solution
Eur. J. Biochem.
204
473-482
1992
Saccharolobus solfataricus
brenda
Ragone, R.; Facchiano, F.; Cacciapuoti, G.; Porcelli, M.; Colonna, G.
Effect of temperature on the propylamine transferase from Sulfolobus solfataricus, an extreme thermophilic archaebacterium. 2. Denaturation and structural stability
Eur. J. Biochem.
204
483-490
1992
Saccharolobus solfataricus
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Knott, J.M.
Biosynthesis of long-chain polyamines by crenarchaeal polyamine synthases from Hyperthermus butylicus and Pyrobaculum aerophilum
FEBS Lett.
583
3519-3524
2009
Hyperthermus butylicus (A2BJU2), Hyperthermus butylicus DSM 5456 (A2BJU2)
brenda
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