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4-hydroxy-3-methyl-2-(E)-butenyl diphosphate + ADP
trans-zeatin riboside 5'-diphosphate + diphosphate
4-hydroxy-3-methyl-2-(E)-butenyl diphosphate + ATP
trans-zeatin riboside 5'-triphosphate + diphosphate
1.4% activity compared to dimethylallyl diphosphate and ADP
-
-
?
dimethylallyl diphosphate + ADP
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
dimethylallyl diphosphate + AMP
diphosphate + N6-(dimethylallyl)adenosine phosphate
dimethylallyl diphosphate + ATP
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
dimethylallyl diphosphate + CDP
?
30% activity compared to ADP
-
-
?
dimethylallyl diphosphate + CTP
?
-
-
-
?
dimethylallyl diphosphate + dADP
?
50% activity compared to ADP
-
-
?
dimethylallyl diphosphate + dATP
?
dimethylallyl diphosphate + diadenosine hexaphosphate
?
-
-
-
-
?
dimethylallyl diphosphate + diadenosine pentaphosphate
?
-
diadenosine pentaphosphate exhibits the highest binding affinity
-
-
?
dimethylallyl diphosphate + diadenosine tetraphosphate
?
-
-
-
-
?
dimethylallyl diphosphate + diadenosine triphosphate
?
-
diadenosine pentaphosphate exhibits the lowest binding affinity and specific activity compared to ATP
-
-
?
dimethylallyl diphosphate + GDP
?
6.4% activity compared to ADP
-
-
?
dimethylallyl diphosphate + GTP
?
-
-
-
?
dimethylallyl diphosphate + UTP
?
-
-
-
?
geranyl diphosphate + ADP
?
1.2% activity compared to dimethylallyl diphosphate and ADP
-
-
?
isopentenyl diphosphate + ADP
?
0.3% activity compared to dimethylallyl diphosphate and ADP
-
-
?
additional information
?
-
4-hydroxy-3-methyl-2-(E)-butenyl diphosphate + ADP
trans-zeatin riboside 5'-diphosphate + diphosphate
-
-
-
-
?
4-hydroxy-3-methyl-2-(E)-butenyl diphosphate + ADP
trans-zeatin riboside 5'-diphosphate + diphosphate
2.2% activity compared to dimethylallyl diphosphate and ADP
-
-
?
dimethylallyl diphosphate + ADP
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
-
-
-
-
?
dimethylallyl diphosphate + ADP
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
-
-
-
?
dimethylallyl diphosphate + ADP
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
-
-
-
?
dimethylallyl diphosphate + ADP
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
-
-
-
-
?
dimethylallyl diphosphate + ADP
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
-
-
-
?
dimethylallyl diphosphate + ADP
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
100% activity
-
-
?
dimethylallyl diphosphate + ADP
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
-
-
-
-
?
dimethylallyl diphosphate + ADP
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
-
-
-
-
?
dimethylallyl diphosphate + AMP
diphosphate + N6-(dimethylallyl)adenosine phosphate
-
low activity with AMP
-
-
?
dimethylallyl diphosphate + AMP
diphosphate + N6-(dimethylallyl)adenosine phosphate
6.6% activity compared to ADP
-
-
?
dimethylallyl diphosphate + AMP
diphosphate + N6-(dimethylallyl)adenosine phosphate
-
low efficiency
-
-
?
dimethylallyl diphosphate + ATP
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
-
-
-
?
dimethylallyl diphosphate + ATP
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
-
-
-
?
dimethylallyl diphosphate + ATP
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
-
highest activity with ATP
-
-
?
dimethylallyl diphosphate + ATP
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
-
-
-
-
?
dimethylallyl diphosphate + ATP
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
-
ATP is the best cosubstrate
-
-
?
dimethylallyl diphosphate + ATP
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
the enzme favors ATP as its ligand
-
-
?
dimethylallyl diphosphate + ATP
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
82% activity compared to ADP
-
-
?
dimethylallyl diphosphate + ATP
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
-
-
-
-
?
dimethylallyl diphosphate + ATP
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
-
-
-
-
?
dimethylallyl diphosphate + dATP
?
the enzyme shows preference for dATP over ADP and 80% activity with dATP compared to ATP
-
-
?
dimethylallyl diphosphate + dATP
?
36% activity compared to ADP
-
-
?
additional information
?
-
-
no activity with AMP and tRNA
-
-
?
additional information
?
-
-
GMP, IMP, CMP, and UMP are not accepted as a substrate
-
-
?
additional information
?
-
-
no activity with NAD+, NADP+ or FAD
-
-
?
additional information
?
-
no activity with farnesyl diphosphate, dAMP, CMP, GMP, IMP, IDP, UMP, UDP, FAD and NADH
-
-
?
additional information
?
-
-
1-hydroxy-2-methyl-2-buten-4-yl 4-diphosphate is not a substrate
-
-
?
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4-hydroxy-3-methyl-2-(E)-butenyl diphosphate + ADP
trans-zeatin riboside 5'-diphosphate + diphosphate
-
-
-
-
?
dimethylallyl diphosphate + ADP
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
dimethylallyl diphosphate + AMP
diphosphate + N6-(dimethylallyl)adenosine phosphate
dimethylallyl diphosphate + ATP
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
additional information
?
-
-
1-hydroxy-2-methyl-2-buten-4-yl 4-diphosphate is not a substrate
-
-
?
dimethylallyl diphosphate + ADP
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
-
-
-
-
?
dimethylallyl diphosphate + ADP
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
-
-
-
?
dimethylallyl diphosphate + ADP
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
-
-
-
?
dimethylallyl diphosphate + ADP
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
-
-
-
-
?
dimethylallyl diphosphate + ADP
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
-
-
-
?
dimethylallyl diphosphate + ADP
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
100% activity
-
-
?
dimethylallyl diphosphate + ADP
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
-
-
-
-
?
dimethylallyl diphosphate + ADP
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
-
-
-
-
?
dimethylallyl diphosphate + AMP
diphosphate + N6-(dimethylallyl)adenosine phosphate
6.6% activity compared to ADP
-
-
?
dimethylallyl diphosphate + AMP
diphosphate + N6-(dimethylallyl)adenosine phosphate
-
low efficiency
-
-
?
dimethylallyl diphosphate + ATP
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
-
-
-
?
dimethylallyl diphosphate + ATP
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
-
-
-
?
dimethylallyl diphosphate + ATP
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
-
highest activity with ATP
-
-
?
dimethylallyl diphosphate + ATP
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
-
-
-
-
?
dimethylallyl diphosphate + ATP
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
-
ATP is the best cosubstrate
-
-
?
dimethylallyl diphosphate + ATP
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
the enzme favors ATP as its ligand
-
-
?
dimethylallyl diphosphate + ATP
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
82% activity compared to ADP
-
-
?
dimethylallyl diphosphate + ATP
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
-
-
-
-
?
dimethylallyl diphosphate + ATP
diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
-
-
-
-
?
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0.759
AMP
-
in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C
0.0065 - 0.0374
dimethylallyl diphosphate
0.0193
ADP
-
in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C
0.0193
ADP
in HEPES (20mM, pH 7.0; 150 mM NaCl, 3 mM dithiothreitol), at 25°C
0.0682
ADP
in 100 mM Tris-HCl buffer, pH 7.0, containing 50 mM KCl, 2 mM MnCl2, 1 mg/ml bovine serum albumin, and 1 mM dithiohtreitol, at 25°C
0.0162
ATP
-
in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C
0.0162
ATP
in HEPES (20mM, pH 7.0; 150 mM NaCl, 3 mM dithiothreitol), at 25°C
0.018
ATP
-
in 25 mM Tris-HCl, pH 7.5, 10 mM MgCl2+, 5 mM 2-mercaptoethanol at 24°C
0.0065
dimethylallyl diphosphate
-
in 25 mM Tris-HCl, pH 7.5, 10 mM MgCl2+, 5 mM 2-mercaptoethanol at 24°C
0.0195
dimethylallyl diphosphate
-
in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C
0.0374
dimethylallyl diphosphate
in 100 mM Tris-HCl buffer, pH 7.0, containing 50 mM KCl, 2 mM MnCl2, 1 mg/ml bovine serum albumin, and 1 mM dithiohtreitol, at 25°C
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0.008
AMP
-
in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C
0.022
ATP
-
in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C
0.027 - 0.035
dimethylallyl diphosphate
0.024
ADP
in 100 mM Tris-HCl buffer, pH 7.0, containing 50 mM KCl, 2 mM MnCl2, 1 mg/ml bovine serum albumin, and 1 mM dithiohtreitol, at 25°C
0.024
ADP
-
in 100 mM TrisHCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C
0.027
dimethylallyl diphosphate
-
in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C
0.035
dimethylallyl diphosphate
in 100 mM Tris-HCl buffer, pH 7.0, containing 50 mM KCl, 2 mM MnCl2, 1 mg/ml bovine serum albumin, and 1 mM dithiohtreitol, at 25°C
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0.0109
AMP
-
in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C
1.34
ATP
-
in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C
0.921 - 1.397
dimethylallyl diphosphate
0.344
ADP
in 100 mM Tris-HCl buffer, pH 7.0, containing 50 mM KCl, 2 mM MnCl2, 1 mg/ml bovine serum albumin, and 1 mM dithiohtreitol, at 25°C
1.238
ADP
-
in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C
0.921
dimethylallyl diphosphate
in 100 mM Tris-HCl buffer, pH 7.0, containing 50 mM KCl, 2 mM MnCl2, 1 mg/ml bovine serum albumin, and 1 mM dithiohtreitol, at 25°C
1.397
dimethylallyl diphosphate
-
in 100 mM Tris-HCl buffer, pH 8.0, containing 50 mM KCl, 10 mM MgCl2, 1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 25°C
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D49A
the mutant almost completely loses the enzyme activity for the prenyl transfer from dimethylallyl diphosphate to ADP
F120A
the mutant almost completely loses the enzyme activity for the prenyl transfer from dimethylallyl diphosphate to ADP
F157A
the mutant almost completely loses the enzyme activity for the prenyl transfer from dimethylallyl diphosphate to ADP
F93A
the mutant almost completely loses the enzyme activity for the prenyl transfer from dimethylallyl diphosphate to ADP
Q255A
the mutant almost completely loses the enzyme activity for the prenyl transfer from dimethylallyl diphosphate to ADP
W159A
the mutant almost completely loses the enzyme activity for the prenyl transfer from dimethylallyl diphosphate to ADP
Y153A
the mutant almost completely loses the enzyme activity for the prenyl transfer from dimethylallyl diphosphate to ADP
Y217A
the mutant almost completely loses the enzyme activity for the prenyl transfer from dimethylallyl diphosphate to ADP
Y54A
the mutant almost completely loses the enzyme activity for the prenyl transfer from dimethylallyl diphosphate to ADP
Y170A
the mutant almost completely loses the enzyme activity for the prenyl transfer from dimethylallyl diphosphate to ADP
Y170A
the mutant retains 84% of wild type activity
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after decapitation, the expression levels of IPT1, IPT3, and IPT5a in nodal stems sharply increase
-
auxin response factor ARF3 directly binds to the promoter of IPT5 and negatively regulates IPT5 expression
enzyme expression is developmentally regulated in kernel tissues and coincides with cytokinin accumulation. The expression gradually increases from 6 to 8 days after pollination (DAP), peaks at 8 DAP, and decreases gradually until 14 DAP before increasing again at later stages (15-34 DAP)
-
enzyme expression levels decrease significantly in pulp within 2 weeks after flowering and remain low
-
isoform IPT1 is down-regulated by the cytokinin 4-(indol-3-yl)butyric acid within 4 h
isoform IPT3 is down-regulated by the cytokinin 4-(indol-3-yl)butyric acid within 4 h
isoform IPT3 is rapidly induced by 1 h treatment with nitrate
isoform IPT3 is upregulated within 2 h after the application of tungstate, methionine sulfoximine or nitrate to N-deprived peach seedlings, and the increase is resistant to pre-treatment of a specific nitrate metabolism inhibitor. Isoform IPT5 in seed increases significantly within 2 weeks after flowering. The enzyme expression also increases significantly during later fruit development in seed
-
isoform IPT5 is down-regulated by the cytokinin 4-(indol-3-yl)butyric acid within 4 h
isoform IPT5 is up-regulated by 4,4'-isopropylidenediphenol (bisphenol A) treatment for 4 h
isoform IPT7 is down-regulated by the cytokinin 4-(indol-3-yl)butyric acid within 4 h
isoform IPT7 is up-regulated by 4,4'-isopropylidenediphenol (bisphenol A) treatment for 4 h
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Takei, K.; Dekishima, Y.; Eguchi, T.; Yamaya, T.; Sakakibara, H.
A new method for enzymatic preparation of isopentenyladenine-type and trans-zeatin-type cytokinins with radioisotope-labeling
J. Plant Res.
116
259-263
2003
Arabidopsis thaliana
brenda
Sakano, Y.; Okada, Y.; Matsunaga, A.; Suwama, T.; Kaneko, T.; Ito, K.; Noguchi, H.; Abe, I.
Molecular cloning, expression, and characterization of adenylate isopentenyltransferase from hop (Humulus lupulus L.)
Phytochemistry
65
2439-2446
2004
Humulus lupulus
brenda
Kakimoto, T.
Identification of plant cytokinin biosynthetic enzymes as dimethylallyl diphosphate:ATP/ADP isopentenyltransferases
Plant Cell Physiol.
42
677-685
2001
Arabidopsis thaliana
brenda
Miyawaki, K.; Matsumoto-Kitano, M.; Kakimoto, T.
Expression of cytokinin biosynthetic isopentenyltransferase genes in Arabidopsis: tissue specificity and regulation by auxin, cytokinin, and nitrate
Plant J.
37
128-138
2004
Arabidopsis thaliana (Q93WC9), Arabidopsis thaliana (Q94ID1), Arabidopsis thaliana (Q94ID2), Arabidopsis thaliana (Q94ID3), Arabidopsis thaliana (Q9C6L1), Arabidopsis thaliana (Q9LJL4), Arabidopsis thaliana (Q9SB60)
brenda
Abe, I.; Tanaka, H.; Abe, T.; Noguchi, H.
Enzymatic formation of unnatural cytokinin analogs by adenylate isopentenyltransferase from mulberry
Biochem. Biophys. Res. Commun.
355
795-800
2007
Morus alba (Q08ET4)
brenda
Miyawaki, K.; Tarkowski, P.; Matsumoto-Kitano, M.; Kato, T.; Sato, S.; Tarkowska, D.; Tabata, S.; Sandberg, G.; Kakimoto, T.
Roles of Arabidopsis ATP/ADP isopentenyltransferases and tRNA isopentenyltransferases in cytokinin biosynthesis
Proc. Natl. Acad. Sci. USA
103
16598-16603
2006
Arabidopsis thaliana (Q93WC9), Arabidopsis thaliana (Q94ID1), Arabidopsis thaliana (Q94ID2), Arabidopsis thaliana (Q94ID3), Arabidopsis thaliana (Q9C6L1), Arabidopsis thaliana (Q9LJL4), Arabidopsis thaliana (Q9SB60), Arabidopsis thaliana
brenda
Brugiere, N.; Humbert, S.; Rizzo, N.; Bohn, J.; Habben, J.E.
A member of the maize isopentenyl transferase gene family, Zea mays isopentenyl transferase 2 (ZmIPT2), encodes a cytokinin biosynthetic enzyme expressed during kernel development. Cytokinin biosynthesis in maize
Plant Mol. Biol.
67
215-229
2008
Zea mays
brenda
Chu, H.M.; Ko, T.P.; Wang, A.H.
Crystal structure and substrate specificity of plant adenylate isopentenyltransferase from Humulus lupulus: distinctive binding affinity for purine and pyrimidine nucleotides
Nucleic Acids Res.
38
1738-1748
2010
Humulus lupulus (Q5GHF7)
brenda
Chu, H.M.; Chen, F.Y.; Ko, T.P.; Wang, A.H.
Binding and catalysis of Humulus lupulus adenylate isopentenyltransferase for the synthesis of isopentenylated diadenosine polyphosphates
FEBS Lett.
584
4083-4088
2010
Humulus lupulus
brenda
Cheng, Z.; Wang, L.; Sun, W.; Zhang, Y.; Zhou, C.; Su, Y.; Li, W.; Sun, T.; Zhao, X.; Li, X.; Cheng, Y.; Zhao, Y.; Xie, Q.; Zhang, X.
Pattern of auxin and cytokinin responses for shoot meristem induction results from the regulation of cytokinin biosynthesis by AUXIN RESPONSE FACTOR3
Plant Physiol.
161
240-251
2013
Arabidopsis thaliana (Q94ID2)
brenda
Min-ji, Li.; Qin-ping, W.; Fu-tian, P.; Wen, Y.; Jing-jing, L.; Yong-fei, Z.
Identification and characterization of ATP/ADP isopentenyltransferases (ATP/ADP PpIPTs) genes in peach
J. Plant Growth Regul.
38
416-430
2018
Prunus persica
-
brenda
Li, M.; Wei, Q.; Xiao, Y.; Peng, F.
The effect of auxin and strigolactone on ATP/ADP isopentenyltransferase expression and the regulation of apical dominance in peach
Plant Cell Rep.
37
1693-1705
2018
Prunus persica
brenda