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dihydroqueuine + tRNAguanine
guanine + tRNAdihydroqueuine
-
-
-
-
?
guanine34 in tRNA + 7-(aminomethyl)-7-deazaguanine
7-(aminomethyl)-7-deazaguanine34 in tRNA + guanine
-
the affinity of the rat liver tRNA-guanine transglycosylase for queuine is much greater than that for 7-(aminomethyl)-7-deazaguanine and guanine
-
-
?
guanine34 in tRNA + 7-aminomethyl-7-carbaguanine
7-aminomethyl-7-carbaguanine34 in tRNA + guanine
very low activity with wild-type enzyme. The mutant enzyme V161C exhibits a significant improvement in preQ1 (7-aminomethyl-7-deazaguanine) recognition
-
-
?
guanine34 in tRNA + queuine
queuine34 in tRNA + guanine
guanine34 in tRNA(Tyr) + queuine
queuine34 in tRNA(Tyr) + guanine
-
-
-
?
tRNAqueuine + guanine
tRNAguanine + queuine
-
-
-
-
?
tRNAqueuine + hypoxanthine
tRNAhypoxanthine + queuine
-
-
-
-
?
tRNAqueuine + xanthine
tRNAxanthine + queuine
-
-
-
-
?
[tRNATyr]queuine + guanine
[tRNATyr]-guanine + queuine
human TGT is composed of a catalytic subunit, QTRT1, and QTRTD1, not USP14. QTRTD1 is the salvage enzyme that generates free queuine from QMP
-
-
?
additional information
?
-
guanine34 in tRNA + queuine
queuine34 in tRNA + guanine
-
-
-
-
?
guanine34 in tRNA + queuine
queuine34 in tRNA + guanine
-
the enzyme replaces a guanine in the anticodon of tRNAs for Asn, Asp, His, and Tyr. In the absence of queuine, this enzyme catalyzes an exchange between free guanine with guanine
-
-
?
guanine34 in tRNA + queuine
queuine34 in tRNA + guanine
-
-
-
-
r
guanine34 in tRNA + queuine
queuine34 in tRNA + guanine
-
-
-
r
guanine34 in tRNA + queuine
queuine34 in tRNA + guanine
-
-
-
?
guanine34 in tRNA + queuine
queuine34 in tRNA + guanine
human tyrosyl tRNA Tyr. The eukaryotic tRNA-guanine transglycosylase enzyme inserts queuine into tRNA via a sequential bi-bi mechanism
-
-
?
guanine34 in tRNA + queuine
queuine34 in tRNA + guanine
-
-
-
?
guanine34 in tRNA + queuine
queuine34 in tRNA + guanine
the enzyme is responsible for the formation of queuosine in the anticodon loop (position 34) of tRNAAsp, tRNAAsn, tRNAHis, and tRNATyr
-
-
?
guanine34 in tRNA + queuine
queuine34 in tRNA + guanine
formation of queuosine in the anticodon loop (position 34) of tRNAAsp, tRNAAsn, tRNAHis, and tRNATyr
-
-
?
guanine34 in tRNA + queuine
queuine34 in tRNA + guanine
-
-
-
-
?
guanine34 in tRNA + queuine
queuine34 in tRNA + guanine
-
in the absence of queuine, this enzyme catalyzes an exchange between free guanine with guanine
-
-
?
guanine34 in tRNA + queuine
queuine34 in tRNA + guanine
-
the enzyme replaces a guanine in the anticodon of tRNAs for Asn, Asp, His, and Tyr. In the absence of queuine, this enzyme catalyzes an exchange between free guanine with guanine
-
-
?
guanine34 in tRNA + queuine
queuine34 in tRNA + guanine
-
-
-
-
?
guanine34 in tRNA + queuine
queuine34 in tRNA + guanine
-
the affinity of the rat liver tRNA-guanine transglycosylase for queuine is much greater than that for 7-(aminomethyl)-7-deazaguanine and guanine
-
-
?
guanine34 in tRNA + queuine
queuine34 in tRNA + guanine
-
-
-
-
?
guanine34 in tRNA + queuine
queuine34 in tRNA + guanine
-
nucleosides in position 36, 37 and 38 influence the efficiency of conversion of G-34 to queuosine-34
-
-
?
guanine34 in tRNA + queuine
queuine34 in tRNA + guanine
-
-
-
?
guanine34 in tRNA + queuine
queuine34 in tRNA + guanine
catalyses a base-exchange reaction that leads to anticodon modifications of certain tRNAs
-
-
?
additional information
?
-
-
-
-
-
?
additional information
?
-
-
key enzyme involved in the incorporation of the modified base queuine into tRNA
-
-
?
additional information
?
-
-
higher expression of the subunit TGT60KD in cancer cells than in normal cells. The expression levels of the TGT60KD subunit regulate enzyme activity and the level of queuosine. TGT60KD plays significant roles in carcinogenesis
-
-
-
additional information
?
-
-
the 60000 Da subunit of the enzyme plays a role in colon carcinogenesis
-
-
-
additional information
?
-
-
the enzyme is required for virulence
-
-
?
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2,4-Diamino-6-hydroxypyrimidine
-
1.2 mM, 52% inhibition of exchange between free guanine with guanine
2,6-diamino-8-[[(1H-imidazol-2-yl)sulfanyl]methyl]quinazolin-4(3H)-one
-
2-butyl-5,6-dihydro-1H-imidazo[4,5-d]pyridazine-4,7-dione
-
3-Deazaguanine
-
0.25 mM, 50% inhibition of exchange between free guanine with guanine
4-(2-phenylethyl)-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
-
5,7-diamino-2,3-dihydro-phthalazine-1,4-dione
-
6-amino-3-(2-phenylethyl)-3,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
-
6-amino-3-benzyl-3,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
-
6-amino-4-(2-phenylethyl)-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
-
6-amino-4-phenyl-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
-
6-amino-4-[2-(4-methylphenyl)ethyl]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
-
6-amino-4-[2-(methoxyphenyl)ethyl]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
-
6-aminoimidazol[4,5-g]quinazolin-8(7H)-one
-
-
6-methylmercaptoguanine
-
0.05 mM, 65% inhibition of exchange between free guanine with guanine
6-thioguanine
-
0.05 mM, 74% inhibition of exchange between free guanine with guanine
7-(aminomethyl)-7-deazaguanine
-
0.01 mM, 28% inhibition
7-Deazaguanine
-
0.06 mM, 27% inhibition of exchange between free guanine with guanine
8-Azaguanine
-
0.05 mM, complete inhibition of exchange between free guanine with guanine
8-Bromoguanine
-
0.05 mM, 86% inhibition of exchange between free guanine with guanine
8-dimethylaminomethyl-7-deazaguanine
-
0.05 mM, 32% inhibition of exchange between free guanine with guanine
adenine
-
0.05 mM, 14% inhibition of exchange between free guanine with guanine
folic acid
-
1.2 mM, complete inhibition of exchange between free guanine with guanine
isocytosine
-
1.2 mM, 52% inhibition of exchange between free guanine with guanine
lumazine
-
1.2 mM, 18% inhibition of exchange between free guanine with guanine
neplanocin A
-
0.1 mM, 45% inhibition of exchange between free guanine with guanine
pterin-6-carboxylic acid
-
1.2 mM, 57% inhibition of exchange between free guanine with guanine
sepiapterin
-
0.13 mM, 33% inhibition of exchange between free guanine with guanine
tetrahydrobiopterin
-
0.02 mM, 82% inhibition of exchange between free guanine with guanine
xanthine
-
for the mutant enzyms D143S and D143T, xanthine is competitive, with respect to guanine
additional information
-
design, synthesis, and in vitro evaluation of novel tricyclic TGT inhibitors based on the lin-benzoguanine scaffold
-
7-methylguanine
-
-
7-methylguanine
-
0.05 mM, 94% inhibition of exchange between free guanine with guanine
biopterin
-
-
biopterin
-
0.02 mM, 39% inhibition of exchange between free guanine with guanine
pterin
-
-
pterin
-
0.0048 mM, 82% inhibition of exchange between free guanine with guanine
queuine
-
0.01 mM, 93% inhibition of exchange between free guanine with guanine
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0.0021
7-(aminomethyl)-7-deazaguanine
-
pH 7.5, 37°C
0.0223 - 0.132
7-aminomethyl-7-carbaguanine
0.00036 - 0.001
guanine34 in tRNA
0.0223
7-aminomethyl-7-carbaguanine
pH 7.3, 37°C, mutant enzyme V161C
0.132
7-aminomethyl-7-carbaguanine
pH 7.3, 37°C, wild-type enzyme
0.00006
guanine
-
pH and temperature not specified in the publication
0.00035
guanine
-
pH 7.3, 37°C, wild-type
0.00038
guanine
wild-type enzyme
0.00087
guanine
-
pH 7.3, 37°C, mutant C145V/T146V
0.001
guanine
-
pH 7.3, 37°C, mutant V217G
0.0013
guanine
mutant enzyme Y106F
0.00209
guanine
-
pH 7.3, 37°C, mutant C145V/T146V/V217G
0.0114
guanine
-
37°C, pH 7.3, mutant enzyme D143A
0.0218
guanine
-
37°C, pH 7.3, mutant enzyme D143N
0.035
guanine
-
pH 7.3, 37°C, mutant C145V/T146V/P147N/V217G
0.057
guanine
-
37°C, pH 7.3, mutant enzyme D143S
0.105
guanine
-
37°C, pH 7.3, mutant enzyme D143T
0.00036
guanine34 in tRNA
mutant enzyme Y106F
0.001
guanine34 in tRNA
wild-type enzyme
0.49
hypoxanthine
-
37°C, pH 7.3, mutant enzyme D143N
0.8 - 11
hypoxanthine
-
37°C, pH 7.3, wild-type enzyme
1.55
hypoxanthine
-
37°C, pH 7.3, mutant enzyme D143A
0.00026
queuine
pH 7.3, 37°C, wild-type
0.00026
queuine
pH 7.3, 37°C, wild-type enzyme
0.00029
queuine
-
pH 7.5, 37°C
0.0006
queuine
recombinant enzyme whose subunits are produced separately and combined in a molar ratio of 1:1 for kinetic measurements, pH and temperature not specified in the publication
0.0023
queuine
-
pH and temperature not specified in the publication
0.0023
queuine
pH and temperature not specified in the publication
0.0026
xanthine
-
37°C, pH 7.3, mutant enzyme D143N
0.013
xanthine
-
37°C, pH 7.3, mutant enzyme D143A
0.224
xanthine
-
37°C, pH 7.3, wild-type enzyme
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0.25
3-Deazaguanine
-
pH and temperature not specified in the publication
0.0028
4-(2-phenylethyl)-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
37°C, pH 7.3
0.0073
6-amino-3-(2-phenylethyl)-3,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
37°C, pH 7.3, Kic
0.015
6-amino-3-benzyl-3,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
37°C, pH 7.3, Kic
0.001
6-amino-4-(2-phenylethyl)-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
37°C, pH 7.3, Kic
0.029
6-amino-4-phenyl-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
37°C, pH 7.3, Kic
0.0069
6-amino-4-[2-(4-methylphenyl)ethyl]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
37°C, pH 7.3, Kic
0.0037
6-amino-4-[2-(methoxyphenyl)ethyl]-1,7-dihydro-8H-imidazo[4,5-g]quinazolin-8-one
-
37°C, pH 7.3, Kic
0.0041 - 0.0079
6-aminoimidazol[4,5-g]quinazolin-8(7H)-one
0.054
7-Deazaguanine
-
pH and temperature not specified in the publication
0.001
7-methylguanine
-
pH and temperature not specified in the publication
0.000023
8-Azaguanine
-
pH and temperature not specified in the publication
0.011
folic acid
-
pH and temperature not specified in the publication
0.1
neplanocin A
-
pH and temperature not specified in the publication
0.000095
queuine
-
pH and temperature not specified in the publication
0.0041
6-aminoimidazol[4,5-g]quinazolin-8(7H)-one
-
37°C, pH 7.3, Kic
0.0079
6-aminoimidazol[4,5-g]quinazolin-8(7H)-one
-
37°C, pH 7.3, Kiu
0.0087
biopterin
pH 7.3, 37°C, wild-type
0.16
biopterin
-
pH 7.3, 37°C, mutant C145V/T146V/V217G
0.55
biopterin
-
pH 7.3, 37°C, mutant C145V/T146V
0.55
biopterin
-
pH 7.3, 37°C, mutant V217G
1.05
biopterin
-
pH 7.3, 37°C, mutant C145V/T146V/P147N/V217G
3
biopterin
-
pH 7.3, 37°C, wild-type
0.00009
pterin
-
pH and temperature not specified in the publication
0.00042
pterin
-
pH 7.3, 37°C, wild-type
0.0009
pterin
-
pH 7.3, 37°C, mutant V217G
0.0013
pterin
-
pH 7.3, 37°C, mutant C145V/T146V/V217G
0.0022
pterin
-
pH 7.3, 37°C, mutant C145V/T146V
0.037
pterin
-
pH 7.3, 37°C, mutant C145V/T146V/P147N/V217G
0.09
xanthine
-
37°C, pH 7.3, mutant enzyme D143T
0.12
xanthine
-
37°C, pH 7.3, mutant enzyme D143S
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Haumont, E.; Droogmans, L.; Grosjean, H.
Enzymatic formation of queuosine and of glycosyl queuosine in yeast tRNAs microinjected into Xenopus laevis oocytes. The effect of the anticodon loop sequence
Eur. J. Biochem.
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1987
Xenopus laevis
brenda
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tRNA-guanine transglycosylase from Escherichia coli: recognition of full-length 'queuine-cognate' tRNAs
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431
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1998
Escherichia coli
brenda
Deshpande, K.L.; Seubert, P.H.; Tillman, D.M.; Farkas, W.R.; Katze, J.R.
Cloning and characterization of cDNA encoding the rabbit tRNA-guanine transglycosylase 60-kilodalton subunit
Arch. Biochem. Biophys.
326
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1996
Oryctolagus cuniculus
brenda
Goodenough-Lashua, D.M.; Garcia, G.A.
tRNA-guanine transglycosylase from E. coli: a ping-pong kinetic mechanism is consistent with nucleophilic catalysis
Bioorg. Chem.
31
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2003
Escherichia coli, Shigella flexneri
brenda
Brenk, R.; Stubbs, M.T.; Heine, A.; Reuter, K.; Klebe, G.
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4
1066-1077
2003
Zymomonas mobilis (P28720)
brenda
Todorov, K.A.; Garcia, G.A.
Role of aspartate 143 in Escherichia coli tRNA-guanine transglycosylase: alteration of heterocyclic substrate specificity
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45
617-625
2006
Escherichia coli
brenda
Chen, Y.C.; Kelly, V.P.; Stachura, S.V.; Garcia, G.A.
Characterization of the human tRNA-guanine transglycosylase: Confirmation of the heterodimeric subunit structure
RNA
16
958-968
2010
Homo sapiens (Q9BXR0), Homo sapiens
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Differential heterocyclic substrate recognition by, and pteridine inhibition of E. coli and human tRNA-guanine transglycosylases
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410
34-39
2011
Escherichia coli, Homo sapiens (Q9BXR0)
brenda
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High-affinity inhibitors of Zymomonas mobilis tRNA-guanine transglycosylase through convergent optimization
Acta Crystallogr. Sect. D
69
1798-1807
2013
Zymomonas mobilis (P28720)
brenda
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Transfer ribonucleic acid guanine transglycosylase isolated from rat liver
Biochemistry
19
395-400
1980
Rattus norvegicus
brenda
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Homodimer architecture of QTRT2, the noncatalytic subunit of the eukaryotic tRNA-guanine transglycosylase
Biochemistry
57
3953-3965
2018
Mus musculus (Q9JMA2 AND B8ZXI1), Mus musculus
brenda
Farkas, W.; Jacobson, K.; Katze, J.
Substrate and inhibitor specificity of tRNA-guanine ribosyltransferase
Biochim. Biophys. Acta
781
64-75
1984
Oryctolagus cuniculus, Mus musculus (Q9JMA2 AND B8ZXI1)
brenda
Johannsson, S.; Neumann, P.; Ficner, R.
Crystal structure of the human tRNA guanine transglycosylase catalytic subunit QTRT1
Biomolecules
8
81
2018
Homo sapiens (Q9BXR0 AND Q9H974), Homo sapiens
brenda
Ishiwata, S.; Ozawa, Y.; Katayama, J.; Kaneko, S.; Shindo, H.; Tomioka, Y.; Ishiwata, T.; Asano, G.; Ikegawa, S.; Mizugaki, M.
Elevated expression level of 60-kDa subunit of tRNA-guanine transglycosylase in colon cancer
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212
113-119
2004
Homo sapiens
-
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Zhang, J.; Lu, R.; Zhang, Y.; Matuszek, Z.; Zhang, W.; Xia, Y.; Pan, T.; Sun, J.
tRNA queuosine modification enzyme modulates the growth and microbiome recruitment to breast tumors
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The eukaryotic tRNA-guanine transglycosylase enzyme inserts queuine into tRNA Via a sequential bi-bi mechanism
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56
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2020
Homo sapiens (Q9BXR0 AND Q9H974)
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Site-directed in vitro replacement of nucleosides in the anticodon loop of tRNA application to the study of structural requirements for queuine insertase activity
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2
1093-1097
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Xenopus laevis
-
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Slight sequence variations of a common fold explain the substrate specificities of tRNA-guanine transglycosylases from the three kingdoms
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416
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1997
Synechocystis sp., Caenorhabditis elegans, Haemophilus influenzae, Helicobacter pylori, Homo sapiens, Mus musculus, Shigella flexneri, Thermotoga maritima
-
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Synthesis, biological evaluation, and crystallographic studies of extended guanine-based (lin-benzoguanine) inhibitors for tRNA-guanine transglycosylase (TGT)
Helv. Chim. Acta
89
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2006
Zymomonas mobilis
-
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tRNA-guanine transglycosylase and guanine-accepting transfer RNAs of Drosophila melanogaster
Insect Biochem.
10
183-188
1980
Drosophila melanogaster, Oryctolagus cuniculus
-
brenda
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129
13-17
2001
Homo sapiens
-
brenda
Walden Jr., T.; Howes, N.; Farkas, W.
Purification and properties of guanine, queuine-tRNA transglycosylase from wheat germ
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257
13218-13222
1982
Triticum aestivum
brenda
Boland, C.; Hayes, P.; Santa-Maria, I.; Nishimura, S.; Kelly, V.
Queuosine formation in eukaryotic tRNA occurs via a mitochondria-localized heteromeric transglycosylase
J. Biol. Chem.
284
18218-18227
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Mus musculus (Q9JMA2 AND B8ZXI1), Mus musculus
brenda
Chen, Y.; Brooks, A.; Goodenough-Lashua, D.; Kittendorf, J.; Showalter, H.; Garcia, G.
Evolution of eukaryal tRNA-guanine transglycosylase Insight gained from the heterocyclic substrate recognition by the wild-type and mutant human and Escherichia coli tRNA-guanine transglycosylases
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39
2834-2844
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Homo sapiens (Q9BXR0 AND Q9H974)
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Investigation of specificity determinants in bacterial tRNA-guanine transglycosylase reveals queuine, the substrate of its eucaryotic counterpart, as inhibitor
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8
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2013
Homo sapiens (Q9BXR0 AND Q9H974)
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