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Information on EC 2.1.1.4 - acetylserotonin O-methyltransferase
for references in articles please use BRENDA:EC2.1.1.4
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EC Tree 2 Transferases
2.1 Transferring one-carbon groups
2.1.1 Methyltransferases
2.1.1.4 acetylserotonin O-methyltransferase
IUBMB Comments
Some other hydroxyindoles also act as acceptor, but more slowly.
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
- 2.1.1.4
- melatonin
-
pineal
- n-acetyltransferase
-
rhythm
- retina
-
circadian
-
nocturnal
-
night
- arylalkylamine
- pinealocytes
-
diurnal
- photoreceptors
-
photoperiodic
-
melatoninergic
-
harderian
-
melatonin-synthesizing
-
daytime
-
nighttime
- 5-hydroxytryptophan
- aa-nat
-
arylalkylamine-n-acetyltransferase
- 5-methoxytryptophan
- 5-methoxytryptamine
- 5-hydroxyindole
- n-acetyl-5-methoxytryptamine
-
pinealectomized
- serotonin-n-acetyltransferase
-
5-hydroxyindoleacetic
- 5-methoxyindoles
- analysis
-
light:dark
- synthesis
-
ganglionectomy
-
extrapineal
Reaction Schemes
showSynonyms
hiomt, hydroxyindole-o-methyltransferase, hydroxyindole o-methyltransferase, n-acetylserotonin methyltransferase, acetylserotonin o-methyltransferase, n-acetylserotonin o-methyltransferase, hydroxyindole-o-methyl transferase, asmt2, acetylserotonin methyltransferase, asmt1, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
acetylserotonin methyltransferase
-
-
-
-
ASMT
HIOMT
hydroxyindole methyltransferase
-
-
-
-
hydroxyindole O-methyltransferase
hydroxyindole-O-methyl transferase
-
-
-
-
hydroxyindole-O-methyltransferase
methyltransferase, acetylserotonin
-
-
-
-
N-acetylserotonin methyltransferase
N-acetylserotonin O-methyltransferase
ASMT
-
-
-
-
HIOMT
-
-
-
-
hydroxyindole O-methyltransferase
-
-
-
-
N-acetylserotonin O-methyltransferase
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + N-acetylserotonin = S-adenosyl-L-homocysteine + melatonin
reaction sequence is ordered bi-bi type
-
S-adenosyl-L-methionine + N-acetylserotonin = S-adenosyl-L-homocysteine + melatonin
reaction sequence is ordered bi-bi type
-
S-adenosyl-L-methionine + N-acetylserotonin = S-adenosyl-L-homocysteine + melatonin
reaction sequence is ordered bi-bi type
-
S-adenosyl-L-methionine + N-acetylserotonin = S-adenosyl-L-homocysteine + melatonin
reaction sequence is ordered bi-bi type
-
S-adenosyl-L-methionine + N-acetylserotonin = S-adenosyl-L-homocysteine + melatonin
reaction sequence is ordered bi-bi type
-
S-adenosyl-L-methionine + N-acetylserotonin = S-adenosyl-L-homocysteine + melatonin
addition of S-adenosyl-L-methionine occuring first followed by N-acetylserotonin
-
S-adenosyl-L-methionine + N-acetylserotonin = S-adenosyl-L-homocysteine + melatonin
addition of S-adenosyl-L-methionine occuring first followed by N-acetylserotonin
-
S-adenosyl-L-methionine + N-acetylserotonin = S-adenosyl-L-homocysteine + melatonin
addition of S-adenosyl-L-methionine occuring first followed by N-acetylserotonin
-
S-adenosyl-L-methionine + N-acetylserotonin = S-adenosyl-L-homocysteine + melatonin
addition of S-adenosyl-L-methionine occuring first followed by N-acetylserotonin
-
S-adenosyl-L-methionine + N-acetylserotonin = S-adenosyl-L-homocysteine + melatonin
addition of S-adenosyl-L-methionine occuring first followed by N-acetylserotonin
-
S-adenosyl-L-methionine + N-acetylserotonin = S-adenosyl-L-homocysteine + melatonin
modeling of the catalytic mechanism
S-adenosyl-L-methionine + N-acetylserotonin = S-adenosyl-L-homocysteine + melatonin
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
methyl group transfer
-
-
-
-
O-methylation
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
BRENDA
MetaCyc
serotonin and melatonin biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:N-acetylserotonin O-methyltransferase
Some other hydroxyindoles also act as acceptor, but more slowly.
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CAS REGISTRY NUMBER
COMMENTARY
9029-77-0
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
4-hydroxy-N-acetyltryptamine + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + N-acetyl-4-methoxytryptamine
-
Substrates: 8% of the activity with N-acetylserotonin
Products: -
Products: -
?
5-hydroxyindoleacetic acid + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + 5-methoxyindoleacetic acid
-
Substrates: 12% of the activity with N-acetylserotonin
Products: -
Products: -
?
bufotenine + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + N,N-dimethyl-5-hydroxytryptamine
N-acetylserotonin + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + N-acetyl-5-methoxytryptamine
N-methylserotonin + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + N-methyl-5-methoxytryptamine
-
Substrates: 9% of the activity with N-acetylserotonin
Products: -
Products: -
?
S-adenosyl-L-methionine + N-acetylserotonin
S-adenosyl-L-homocysteine + N-acetyl-5-methoxytryptamine
bufotenine + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + N,N-dimethyl-5-hydroxytryptamine
-
Substrates: -
Products: -
Products: -
?
bufotenine + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + N,N-dimethyl-5-hydroxytryptamine
-
Substrates: 14% of the activity with N-acetylserotonin
Products: -
Products: -
?
bufotenine + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + N,N-dimethyl-5-hydroxytryptamine
-
Substrates: -
Products: -
Products: -
?
N-acetylserotonin + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + N-acetyl-5-methoxytryptamine
-
Substrates: formation of the hormone melatonin
Products: -
Products: -
?
N-acetylserotonin + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + N-acetyl-5-methoxytryptamine
-
Substrates: formation of the hormone melatonin
Products: -
Products: -
?
N-acetylserotonin + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + N-acetyl-5-methoxytryptamine
-
Substrates: formation of the hormone melatonin
Products: -
Products: -
?
N-acetylserotonin + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + N-acetyl-5-methoxytryptamine
-
Substrates: -
Products: -
Products: -
?
N-acetylserotonin + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + N-acetyl-5-methoxytryptamine
-
Substrates: formation of the hormone melatonin
Products: -
Products: -
?
N-acetylserotonin + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + N-acetyl-5-methoxytryptamine
-
Substrates: formation of the hormone melatonin
Products: -
Products: -
?
N-acetylserotonin + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + N-acetyl-5-methoxytryptamine
-
Substrates: -
Products: -
Products: -
?
N-acetylserotonin + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + N-acetyl-5-methoxytryptamine
-
Substrates: formation of the hormone melatonin
Products: -
Products: -
?
S-adenosyl-L-methionine + N-acetylserotonin
S-adenosyl-L-homocysteine + melatonin
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + N-acetylserotonin
S-adenosyl-L-homocysteine + melatonin
-
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + N-acetylserotonin
S-adenosyl-L-homocysteine + melatonin
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + N-acetylserotonin
S-adenosyl-L-homocysteine + melatonin
-
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + N-acetylserotonin
S-adenosyl-L-homocysteine + melatonin
-
Substrates: the enzyme is involved in melatonin biosynthesis in egg yolk, overview
Products: -
Products: -
?
S-adenosyl-L-methionine + N-acetylserotonin
S-adenosyl-L-homocysteine + melatonin
-
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + N-acetylserotonin
S-adenosyl-L-homocysteine + melatonin
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + N-acetylserotonin
S-adenosyl-L-homocysteine + melatonin
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + N-acetylserotonin
S-adenosyl-L-homocysteine + melatonin
-
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + N-acetylserotonin
S-adenosyl-L-homocysteine + melatonin
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + N-acetylserotonin
S-adenosyl-L-homocysteine + melatonin
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + N-acetylserotonin
S-adenosyl-L-homocysteine + N-acetyl-5-methoxytryptamine
-
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + N-acetylserotonin
S-adenosyl-L-homocysteine + N-acetyl-5-methoxytryptamine
-
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + N-acetylserotonin
S-adenosyl-L-homocysteine + N-acetyl-5-methoxytryptamine
-
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + N-acetylserotonin
S-adenosyl-L-homocysteine + N-acetyl-5-methoxytryptamine
Frog
-
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + N-acetylserotonin
S-adenosyl-L-homocysteine + N-acetyl-5-methoxytryptamine
-
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + N-acetylserotonin
S-adenosyl-L-homocysteine + N-acetyl-5-methoxytryptamine
-
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + N-acetylserotonin
S-adenosyl-L-homocysteine + N-acetyl-5-methoxytryptamine
-
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + N-acetylserotonin
S-adenosyl-L-homocysteine + N-acetyl-5-methoxytryptamine
-
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + N-acetylserotonin
S-adenosyl-L-homocysteine + N-acetyl-5-methoxytryptamine
-
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + N-acetylserotonin
S-adenosyl-L-homocysteine + N-acetyl-5-methoxytryptamine
-
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + N-acetylserotonin
S-adenosyl-L-homocysteine + N-acetyl-5-methoxytryptamine
-
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + serotonin
S-adenosyl-L-homocysteine + 5-methoxytryptamine
Substrates: enzyme AtASMT also O-methylates serotonin to produce 5-methoxytryptamine
Products: -
Products: -
?
S-adenosyl-L-methionine + serotonin
S-adenosyl-L-homocysteine + 5-methoxytryptamine
Substrates: enzyme AtASMT also O-methylates serotonin to produce 5-methoxytryptamine
Products: -
Products: -
?
serotonin + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + 5-methoxytryptamine
-
Substrates: -
Products: -
Products: -
?
serotonin + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + 5-methoxytryptamine
-
Substrates: 7% of the activity with N-acetylserotonin
Products: -
Products: -
?
serotonin + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + 5-methoxytryptamine
-
Substrates: -
Products: -
Products: -
?
additional information
?
-
Substrates: caffeic acid O-methyltransferase (COMT), EC 2.1.1.68, methylates N-acetylserotonin into melatonin, it has N-acetylserotonin O-methyltransferase (ASMT) activity. COMT is a multifunctional enzyme methylating a diverse array of substrates, including phenylpropanoids, flavonoids, and arylalkylamines
Products: -
Products: -
?
additional information
?
-
-
Substrates: caffeic acid O-methyltransferase (COMT), EC 2.1.1.68, methylates N-acetylserotonin into melatonin, it has N-acetylserotonin O-methyltransferase (ASMT) activity. COMT is a multifunctional enzyme methylating a diverse array of substrates, including phenylpropanoids, flavonoids, and arylalkylamines
Products: -
Products: -
?
additional information
?
-
Substrates: the AtASMT protein catalyzes the conversion of N-acetylserotonin to melatonin and serotonin to 5-methoxytryptamine, respectively. The enzyme exhibits no caffeic acid O-methyltransferase activity, suggesting that its function is highly specific to melatonin synthesis
Products: -
Products: -
?
additional information
?
-
-
Substrates: the AtASMT protein catalyzes the conversion of N-acetylserotonin to melatonin and serotonin to 5-methoxytryptamine, respectively. The enzyme exhibits no caffeic acid O-methyltransferase activity, suggesting that its function is highly specific to melatonin synthesis
Products: -
Products: -
?
additional information
?
-
Substrates: caffeic acid is no substrate
Products: -
Products: -
?
additional information
?
-
-
Substrates: caffeic acid is no substrate
Products: -
Products: -
?
additional information
?
-
Substrates: caffeic acid O-methyltransferase (COMT), EC 2.1.1.68, methylates N-acetylserotonin into melatonin, it has N-acetylserotonin O-methyltransferase (ASMT) activity. COMT is a multifunctional enzyme methylating a diverse array of substrates, including phenylpropanoids, flavonoids, and arylalkylamines
Products: -
Products: -
?
additional information
?
-
Substrates: the AtASMT protein catalyzes the conversion of N-acetylserotonin to melatonin and serotonin to 5-methoxytryptamine, respectively. The enzyme exhibits no caffeic acid O-methyltransferase activity, suggesting that its function is highly specific to melatonin synthesis
Products: -
Products: -
?
additional information
?
-
Substrates: caffeic acid is no substrate
Products: -
Products: -
?
additional information
?
-
-
Substrates: absolute requirement for S-adenosylmethionine
Products: -
Products: -
?
additional information
?
-
-
Substrates: bovine enzyme has high specificity towards N-acetylserotonin, chicken enzyme methylates N-acetylserotonin and to some extent serotonin and bufotenine
Products: -
Products: -
?
additional information
?
-
-
Substrates: absolute requirement for S-adenosylmethionine
Products: -
Products: -
?
additional information
?
-
-
Substrates: bovine enzyme has high specificity towards N-acetylserotonin, chicken enzyme methylates N-acetylserotonin and to some extent serotonin and bufotenine
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme also catalyzes the conversion of 5-hydroxyindoleacetate to 5-methoxyindoleacetate
Products: -
Products: -
?
additional information
?
-
-
Substrates: absolute requirement for S-adenosylmethionine
Products: -
Products: -
?
additional information
?
-
Substrates: caffeic acid O-methyltransferase (COMT), EC 2.1.1.68, methylates N-acetylserotonin into melatonin, it has N-acetylserotonin O-methyltransferase (ASMT) activity. The ASMT activity (Vmax/Km) of OsCOMT is 609fold higher than that of rice ASMT1
Products: -
Products: -
?
additional information
?
-
-
Substrates: caffeic acid O-methyltransferase (COMT), EC 2.1.1.68, methylates N-acetylserotonin into melatonin, it has N-acetylserotonin O-methyltransferase (ASMT) activity. The ASMT activity (Vmax/Km) of OsCOMT is 609fold higher than that of rice ASMT1
Products: -
Products: -
?
additional information
?
-
-
Substrates: activity is controlled by the endogenous clock
Products: -
Products: -
?
additional information
?
-
-
Substrates: in pineal glands isolated in the morning 4 h magnetic field exposure changes melatonin release by affecting the signal transduction pathway leading from the norepinephrine receptor to N-acetyltransferase and hydroxyindole-O-methyltransferase and not via a direct effect at the enzyme levels
Products: -
Products: -
?
additional information
?
-
-
Substrates: in pineal glands isolated in the morning 4 h magnetic field exposure changes melatonin release by affecting the signal transduction pathway leading from the norepinephrine receptor to N-acetyltransferase and hydroxyindole-O-methyltransferase and not via a direct effect at the enzyme levels
Products: -
Products: -
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
N-acetylserotonin + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + N-acetyl-5-methoxytryptamine
N-acetylserotonin + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + N-acetyl-5-methoxytryptamine
-
Substrates: formation of the hormone melatonin
Products: -
Products: -
?
N-acetylserotonin + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + N-acetyl-5-methoxytryptamine
-
Substrates: formation of the hormone melatonin
Products: -
Products: -
?
N-acetylserotonin + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + N-acetyl-5-methoxytryptamine
-
Substrates: formation of the hormone melatonin
Products: -
Products: -
?
N-acetylserotonin + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + N-acetyl-5-methoxytryptamine
-
Substrates: -
Products: -
Products: -
?
N-acetylserotonin + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + N-acetyl-5-methoxytryptamine
-
Substrates: formation of the hormone melatonin
Products: -
Products: -
?
N-acetylserotonin + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + N-acetyl-5-methoxytryptamine
-
Substrates: formation of the hormone melatonin
Products: -
Products: -
?
N-acetylserotonin + S-adenosyl-L-methionine
S-adenosyl-L-homocysteine + N-acetyl-5-methoxytryptamine
-
Substrates: formation of the hormone melatonin
Products: -
Products: -
?
S-adenosyl-L-methionine + N-acetylserotonin
S-adenosyl-L-homocysteine + melatonin
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + N-acetylserotonin
S-adenosyl-L-homocysteine + melatonin
-
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + N-acetylserotonin
S-adenosyl-L-homocysteine + melatonin
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + N-acetylserotonin
S-adenosyl-L-homocysteine + melatonin
-
Substrates: the enzyme is involved in melatonin biosynthesis in egg yolk, overview
Products: -
Products: -
?
S-adenosyl-L-methionine + N-acetylserotonin
S-adenosyl-L-homocysteine + melatonin
-
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + N-acetylserotonin
S-adenosyl-L-homocysteine + melatonin
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + N-acetylserotonin
S-adenosyl-L-homocysteine + melatonin
-
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + N-acetylserotonin
S-adenosyl-L-homocysteine + melatonin
Substrates: -
Products: -
Products: -
?
S-adenosyl-L-methionine + serotonin
S-adenosyl-L-homocysteine + 5-methoxytryptamine
Substrates: enzyme AtASMT also O-methylates serotonin to produce 5-methoxytryptamine
Products: -
Products: -
?
S-adenosyl-L-methionine + serotonin
S-adenosyl-L-homocysteine + 5-methoxytryptamine
Substrates: enzyme AtASMT also O-methylates serotonin to produce 5-methoxytryptamine
Products: -
Products: -
?
additional information
?
-
Substrates: caffeic acid O-methyltransferase (COMT), EC 2.1.1.68, methylates N-acetylserotonin into melatonin, it has N-acetylserotonin O-methyltransferase (ASMT) activity. COMT is a multifunctional enzyme methylating a diverse array of substrates, including phenylpropanoids, flavonoids, and arylalkylamines
Products: -
Products: -
?
additional information
?
-
-
Substrates: caffeic acid O-methyltransferase (COMT), EC 2.1.1.68, methylates N-acetylserotonin into melatonin, it has N-acetylserotonin O-methyltransferase (ASMT) activity. COMT is a multifunctional enzyme methylating a diverse array of substrates, including phenylpropanoids, flavonoids, and arylalkylamines
Products: -
Products: -
?
additional information
?
-
Substrates: the AtASMT protein catalyzes the conversion of N-acetylserotonin to melatonin and serotonin to 5-methoxytryptamine, respectively. The enzyme exhibits no caffeic acid O-methyltransferase activity, suggesting that its function is highly specific to melatonin synthesis
Products: -
Products: -
?
additional information
?
-
-
Substrates: the AtASMT protein catalyzes the conversion of N-acetylserotonin to melatonin and serotonin to 5-methoxytryptamine, respectively. The enzyme exhibits no caffeic acid O-methyltransferase activity, suggesting that its function is highly specific to melatonin synthesis
Products: -
Products: -
?
additional information
?
-
Substrates: caffeic acid O-methyltransferase (COMT), EC 2.1.1.68, methylates N-acetylserotonin into melatonin, it has N-acetylserotonin O-methyltransferase (ASMT) activity. COMT is a multifunctional enzyme methylating a diverse array of substrates, including phenylpropanoids, flavonoids, and arylalkylamines
Products: -
Products: -
?
additional information
?
-
Substrates: the AtASMT protein catalyzes the conversion of N-acetylserotonin to melatonin and serotonin to 5-methoxytryptamine, respectively. The enzyme exhibits no caffeic acid O-methyltransferase activity, suggesting that its function is highly specific to melatonin synthesis
Products: -
Products: -
?
additional information
?
-
-
Substrates: the enzyme also catalyzes the conversion of 5-hydroxyindoleacetate to 5-methoxyindoleacetate
Products: -
Products: -
?
additional information
?
-
Substrates: caffeic acid O-methyltransferase (COMT), EC 2.1.1.68, methylates N-acetylserotonin into melatonin, it has N-acetylserotonin O-methyltransferase (ASMT) activity. The ASMT activity (Vmax/Km) of OsCOMT is 609fold higher than that of rice ASMT1
Products: -
Products: -
?
additional information
?
-
-
Substrates: caffeic acid O-methyltransferase (COMT), EC 2.1.1.68, methylates N-acetylserotonin into melatonin, it has N-acetylserotonin O-methyltransferase (ASMT) activity. The ASMT activity (Vmax/Km) of OsCOMT is 609fold higher than that of rice ASMT1
Products: -
Products: -
?
additional information
?
-
-
Substrates: in pineal glands isolated in the morning 4 h magnetic field exposure changes melatonin release by affecting the signal transduction pathway leading from the norepinephrine receptor to N-acetyltransferase and hydroxyindole-O-methyltransferase and not via a direct effect at the enzyme levels
Products: -
Products: -
?
additional information
?
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Substrates: in pineal glands isolated in the morning 4 h magnetic field exposure changes melatonin release by affecting the signal transduction pathway leading from the norepinephrine receptor to N-acetyltransferase and hydroxyindole-O-methyltransferase and not via a direct effect at the enzyme levels
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
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ASMT-specific activity is not altered by the addition of EDTA or the divalent cation Mg2+, suggesting that Oryza sativa ASMT is a cation-independent O-methyltransferase
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
caffeic acid
in vitro N-acetylserotonin methyltransferase activity is significantly and competitively inhibited by caffeic acid, a substrate of the caffeic acid O-methyltransferase, 73% inhibition at 0.01 mM, 93% at 0.1 mM
cysteamine
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rat enzyme inhibited to 50% at 0.128 mM, bovine and ovine enzyme not
fisetin
0.1 mM, strong inhibition of N-acetylserotonin methyltransferase activity
kaempferol
0.1 mM, strong inhibition of N-acetylserotonin methyltransferase activity
L-glutamate
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inhibition is reversible, dose-dependent, no inhibition of mRNA expression
luteolin
0.1 mM, strong inhibition of N-acetylserotonin methyltransferase activity
morin
0.1 mM, strong inhibition of N-acetylserotonin methyltransferase activity
myricetin
0.1 mM, strong inhibition of N-acetylserotonin methyltransferase activity
quercetin
in vitro N-acetylserotonin methyltransferase activity is significantly and competitively inhibited by quercetin, a substrate of the caffeic acid O-methyltransferase, in vivo production of melatonin is significantly inhibited by quercetin in 4-week-old detached rice leaves, 97% inhibition at 0.01 mM, complete inhibition at over 0.05 mM
quercetin
0.1 mM, strong inhibition of N-acetylserotonin methyltransferase activity
additional information
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various amine compounds, including tyramine, tryptamine, N-acetyltryptamine, and 5-methoxytryptamine show no or poor inhibition of the enzyme. The enzyme does not show substrate inhibition at high N-acetylserotonin concentrations
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additional information
all of the flavonoids tested greatly inhibit the ASMT activity of COMT, and thus the melatonin biosynthetic activity, with the exception of catechin
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additional information
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not inhibitory: (2S,2'R,3'R)-2-(2',3'-dicarboxycyclopropyl)glycine
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
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retinoic acid stereoisomers induce an increase in activity and mRNA level
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Breast Neoplasms
ASMT Regulates Tumor Metastasis Through the Circadian Clock System in Triple-Negative Breast Cancer.
Breast Neoplasms
Cytoguardin: A Tryptophan Metabolite against Cancer Growth and Metastasis.
Carcinogenesis
Induction and growth of mammary tumors after superior cervical ganglionectomy in sighted and blinded-anosmic rats.
Choriocarcinoma
Human placental trophoblasts synthesize melatonin and express its receptors.
Colitis, Ulcerative
Evaluation of enterochromaffin cells and melatonin secretion exponents in ulcerative colitis.
Ganglion Cysts
Melatonin and 5-methoxytryptophol (5-ML) in nervous and/or neurosensory structures of a gastropod mollusc (Helix aspersa maxima): synthesis and diurnal rhythms.
Medulloblastoma
Expression of hydroxyindole-O-methyltransferase enzyme in the human central nervous system and in pineal parenchymal cell tumors.
Neoplasms
ASMT Regulates Tumor Metastasis Through the Circadian Clock System in Triple-Negative Breast Cancer.
Neoplasms
Demonstration of hydroxyindole-O-methyltransferase (HIOMT) mRNA expression in pineal parenchymal tumors: histochemical in situ hybridization.
Neoplasms
Effect of TNF-alpha on the melatonin synthetic pathway in the rat pineal gland: basis for a 'feedback' of the immune response on circadian timing.
Neoplasms
Expression of hydroxyindole-O-methyltransferase enzyme in the human central nervous system and in pineal parenchymal cell tumors.
Neoplasms
Histological features and expression of enzymes implicated in melatonin synthesis in pineal parenchymal tumours and in cultured tumoural pineal cells.
Neoplasms
Inhibitory effects of superior cervical ganglionectomy on dimethylbenz(a)anthracene-induced mammary tumors in the rat.
Neoplasms
Modification of hydroxyindole-O-methyltransferase activity in experimental pineocytomas induced in hamsters by a human papovavirus (JC).
Neoplasms
Restoration of hydroxyindole O-methyltransferase levels in human cancer cells induces a tryptophan-metabolic switch and attenuates cancer progression.
Neuroectodermal Tumors, Primitive
Expression of hydroxyindole-O-methyltransferase enzyme in the human central nervous system and in pineal parenchymal cell tumors.
Pinealoma
DEMONSTRATION OF HYDROXYINDOLE-O-METHYL TRANSFERASE, MELATONIN, AND SEROTONIN IN A METASTATIC PARENCHYMATOUS PINEALOMA.
Pinealoma
Demonstration of hydroxyindole-O-methyltransferase (HIOMT) mRNA expression in pineal parenchymal tumors: histochemical in situ hybridization.
Pinealoma
Experimental and spontaneous pineal tumors: findings relating to endocrine and oncogenic factors and mechanisms.
Pinealoma
Immunoreactive S-antigen in cerebrospinal fluid: a marker of pineal parenchymal tumors?
Pinealoma
Microarray analysis reveals differential gene expression patterns in tumors of the pineal region.
Pinealoma
Modification of hydroxyindole-O-methyltransferase activity in experimental pineocytomas induced in hamsters by a human papovavirus (JC).
Retinitis Pigmentosa
Immunocytochemistry of retinitis pigmentosa retinas: localization of HIOMT immunoreactivity in surviving photoreceptor cells.
Retinoblastoma
Cyclic AMP and butyrate modulate melatonin synthesis in Y79 human retinoblastoma cells.
Retinoblastoma
Human hydroxyindole-O-methyltransferase in pineal gland, retina and Y79 retinoblastoma cells.
Retinoblastoma
Hydroxyindole-O-methyltransferase in Y-79 human retinoblastoma cells: effect of cell attachment.
Retinoblastoma
Regulation of AA-NAT and HIOMT gene expression by butyrate and cyclic AMP in Y79 human retinoblastoma cells.
Retinoblastoma
Retinoic acid increases hydroxyindole-O-methyltransferase activity and mRNA in human Y-79 retinoblastoma cells.
Vitamin A Deficiency
Regulation of hydroxyindole-O-methyltransferase gene expression in Japanese quail (Coturnix coturnix japonica).
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.864
N-acetylserotonin
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recombinant enzyme, pH not specified in the publication, 30°C
additional information
additional information