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S-adenosyl-L-methionine + guanine748 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
S-adenosyl-L-methionine + guanine748 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methyluanine748 in 23S rRNA
in vivo TlrB modifies nucleotide G748 within helix 35 of 23S rRNA
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S-adenosyl-L-methionine + guanine748 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
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Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate
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S-adenosyl-L-methionine + guanine748 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
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Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate
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?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
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Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Enterococcus faecalis shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro
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?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
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Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Lactobacillus plantarum shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro
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?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
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Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Lactococcus lactis shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro
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?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate
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-
?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Bacillus megaterium shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro
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?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
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Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Nocardia corynebacteroides shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro
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S-adenosyl-L-methionine + guanine748 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
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S-adenosyl-L-methionine + guanine748 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate
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?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
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progressive truncation of the rRNA substrate indicates that multiple contacts occur between RlmAII and nucleotides in stemloops 33, 34 and 35. RlmAII appears to recognize its rRNA target through specific surface shape complementarity at the junction formed by these three helices
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S-adenosyl-L-methionine + guanine748 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
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RlmAII makes multiple footprint contacts with nucleotides in stemloops 33, 34 and 35, and does not interact elsewhere in the rRNA. Binding of RlmAII to the rRNA is dependent on the cofactor S-adenosylmethionine (or S-adenosylhomocysteine). RlmAII interacts with the same rRNA region as the orthologous enzyme RlmAI that methylates at nucleotide G745
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S-adenosyl-L-methionine + guanine748 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
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the enzyme methylates 23S rRNA at nucleotide G748, which is situated in a stem-loop (hairpin 35) at the macrolide binding site of the ribosome. Binding of RlmAII methyltransferase induces chemical shift changes in the RNA. The conformation of hairpin 35 that interacts with RlmAII is radically different from the structure this hairpin adopts within the 50S subunit
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S-adenosyl-L-methionine + guanine748 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
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S-adenosyl-L-methionine + guanine748 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
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Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Streptococcus thermophilus shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro
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?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate
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?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
the methyltransferase RlmAII (TlrB) confers resistance to the macrolide antibiotic tylosin in the drug-producing strain Streptomyces fradiae. The resistance conferred by RlmAII is highly specific for tylosin, and no resistance is conferred to other macrolide drugs, or to lincosamide and streptogramin B drugs that bind to the same region on the bacterial ribosome
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S-adenosyl-L-methionine + guanine748 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
purified recombinant TlrB modifies G748 in 23S rRNA as well as in a 74 nucleotide RNA containing helix 35 and surrounding structures
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S-adenosyl-L-methionine + guanine748 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
the methylation site of RlmAII is identified by liquid chromatography/electrospray ionization mass spectrometry as the N-1 position of 23S rRNA nucleotide G748
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
S-adenosyl-L-methionine + guanine748 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
S-adenosyl-L-methionine + guanine748 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methyluanine748 in 23S rRNA
in vivo TlrB modifies nucleotide G748 within helix 35 of 23S rRNA
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?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
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Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate
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?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
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Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate
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?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
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Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Enterococcus faecalis shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro
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?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
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Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Lactobacillus plantarum shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro
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?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
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Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Lactococcus lactis shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro
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?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
-
Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate
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?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
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Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Bacillus megaterium shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro
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?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
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Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Nocardia corynebacteroides shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro
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S-adenosyl-L-methionine + guanine748 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
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S-adenosyl-L-methionine + guanine748 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
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Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate
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S-adenosyl-L-methionine + guanine748 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
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S-adenosyl-L-methionine + guanine748 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
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Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Streptococcus thermophilus shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro
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?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate
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?
S-adenosyl-L-methionine + guanine748 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
the methyltransferase RlmAII (TlrB) confers resistance to the macrolide antibiotic tylosin in the drug-producing strain Streptomyces fradiae. The resistance conferred by RlmAII is highly specific for tylosin, and no resistance is conferred to other macrolide drugs, or to lincosamide and streptogramin B drugs that bind to the same region on the bacterial ribosome
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Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
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brenda
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
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brenda
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
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brenda
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
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brenda
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
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brenda
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
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brenda
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
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brenda
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
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brenda
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brenda
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
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brenda
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brenda
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
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brenda
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UniProt
brenda
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII
UniProt
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Liu, M.; Douthwaite, S.
Methylation at nucleotide G745 or G748 in 23S rRNA distinguishes gram-negative from gram-positive bacteria
Mol. Microbiol.
44
195-204
2002
Priestia megaterium, Bacillus subtilis, Corynebacterium glutamicum, Streptococcus pneumoniae, Enterococcus faecalis, Lactiplantibacillus plantarum, Lactococcus lactis, Streptococcus thermophilus, Micromonospora griseorubida, Rhodococcus corynebacterioides, Streptomyces fradiae (Q9S1M6), Streptomyces fradiae
brenda
Lebars, I.; Yoshizawa, S.; Stenholm, A.R.; Guittet, E.; Douthwaite, S.; Fourmy, D.
Structure of 23S rRNA hairpin 35 and its interaction with the tylosin-resistance methyltransferase RlmAII
EMBO J.
22
183-192
2003
Streptococcus pneumoniae
brenda
Douthwaite, S.; Crain, P.F.; Liu, M.; Poehlsgaard, J.
The tylosin-resistance methyltransferase RlmAII (TlrB) modifies the N-1 position of 23S rRNA nucleotide G748
J. Mol. Biol.
337
1073-1077
2004
Streptomyces fradiae (Q9S1M6), Streptomyces fradiae
brenda
Douthwaite, S.; Jakobsen, L.; Yoshizawa, S.; Fourmy, D.
Interaction of the tylosin-resistance methyltransferase RlmA II at its rRNA target differs from the orthologue RlmA I
J. Mol. Biol.
378
969-975
2008
Streptococcus pneumoniae
brenda
Lebars, I.; Husson, C.; Yoshizawa, S.; Douthwaite, S.; Fourmy, D.
Recognition elements in rRNA for the tylosin resistance methyltransferase RlmA(II)
J. Mol. Biol.
372
525-534
2007
Streptococcus pneumoniae
brenda
Liu, M.; Kirpekar, F.; van Wezel, G.P.; Douthwaite, S.
The tylosin resistance gene tlrB of Streptomyces fradiae encodes a methyltransferase that targets G748 in 23S rRNA
Mol. Microbiol.
37
811-820
2000
Streptomyces fradiae (Q9S1M6)
brenda
Takaya, A.; Sato, Y.; Shoji, T.; Yamamoto, T.
Methylation of 23S rRNA nucleotide G748 by RlmAII methyltransferase renders Streptococcus pneumoniae telithromycin susceptible
Antimicrob. Agents Chemother.
57
3789-3796
2013
Streptococcus pneumoniae, Streptococcus pneumoniae S1
brenda
Shoji, T.; Takaya, A.; Sato, Y.; Kimura, S.; Suzuki, T.; Yamamoto, T.
RlmCD-mediated U747 methylation promotes efficient G748 methylation by methyltransferase RlmAII in 23S rRNA in Streptococcus pneumoniae; interplay between two rRNA methylations responsible for telithromycin susceptibility
Nucleic Acids Res.
43
8964-8972
2015
Streptococcus pneumoniae, Streptococcus pneumoniae S1
brenda