The enzyme catalyses the last step in menaquinone biosynthesis. It is able to accept substrates with varying polyprenyl side chain length (the chain length is determined by polyprenyl diphosphate synthase). The enzyme from Escherichia coli also catalyses the conversion of 2-methoxy-6-octaprenyl-1,4-benzoquinone to 5-methoxy-2-methyl-3-octaprenyl-1,4-benzoquinone during the biosynthesis of ubiquinone . The enzyme probably acts on menaquinol rather than menaquinone.
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The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
The enzyme catalyses the last step in menaquinone biosynthesis. It is able to accept substrates with varying polyprenyl side chain length (the chain length is determined by polyprenyl diphosphate synthase)[1]. The enzyme from Escherichia coli also catalyses the conversion of 2-methoxy-6-octaprenyl-1,4-benzoquinone to 5-methoxy-2-methyl-3-octaprenyl-1,4-benzoquinone during the biosynthesis of ubiquinone [4]. The enzyme probably acts on menaquinol rather than menaquinone.
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Tuberculosis
A Novel Small-Molecule Inhibitor of the Mycobacterium tuberculosis Demethylmenaquinone Methyltransferase MenG Is Bactericidal to Both Growing and Nutritionally Deprived Persister Cells.
ORF-1, ORF-2, and ORF-3 genes, designated heps-1, menG, and heps-2, respectively, form another cluster involved in menaquinone biosynthesis in addition to the cluster of menB, menC, menD, and menE already identified in the Bacillus subtilis and Escherichia coli chromosomes
in the uniE-lacking mutant strain AN70, a lack of ubiquinone occurs due to the missing methylation reaction. The mutant also contains only demethylmenaquinone, no menaquinone. Mutant respiration of DMSO is increased, while the respiration of fumarate is reduced compared to the wild-type enzyme
in the uniE-lacking mutant strain AN70, a lack of ubiquinone occurs due to the missing methylation reaction. The mutant also contains only demethylmenaquinone, no menaquinone. Mutant respiration of DMSO is increased, while the respiration of fumarate is reduced compared to the wild-type enzyme
the enzyme catalyzes a step in the menaquinone pathway as well as the methylation step in ubiquinone pathway, overview. Menaquinne activates the respiration of fumarate, trimethylamine N-oxide, nitrate, and DMSO
demethylmenaquinone methyltransferase catalyzes the terminal step of menaquinone biosynthesis, overview. MK-7 and UQ-10 are accumulated in 7653R in microaerobic respiration in strain 7653R
the demethylmenaquinone methyltransferase or 2-heptaprenyl-1,4-naphthoquinone synthase participates in the terminal step of the menaquinone biosynthesis
demethylmenaquinone methyltransferase catalyzes the terminal step of menaquinone biosynthesis, overview. MK-7 and UQ-10 are accumulated in 7653R in microaerobic respiration in strain 7653R
the enzyme catalyzes a step in the menaquinone pathway as well as the methylation step in ubiquinone pathway, overview. Menaquinne activates the respiration of fumarate, trimethylamine N-oxide, nitrate, and DMSO
mutant defective in the demthylmenaquinone methyltransferase activity expresses the same formate-dependent nitrite reduction activity as the parental strain. Either menaquinone or ubiquinone, but not demethylmenaquinone, can transfer electrons to a third cytochrome-c-dependent electron transfer chain, the periplasmic nitrate reductase
mutant ubiE lacks ubiquinone due to defect in a specific methylation step of ubiquinone synthesis. Synthesis of menaquinone from demethylmenaquinone depends on the same gene ubiE. Mutant contains only demethylmenaquinone, but not menaquinone. Strain is able to grow with fumarate, trimethylamine N-oxide and dimethylsulfoxide, but not with nitrate as electron acceptor. Anaerobic respiration with fumarate and trimethylamine are catalyzed at 69% and 74% of wild-type rates, respectively. Dimethylsulfoxide respiration is reduced to 38% of wild-type, and nitrate respiration is below 8%
strains containing either a disruption or point mutation G142D in ubiE accumulate 2-octaprenyl-6-methoxy-1,4-benzoquinone and demethylmenaquinone as predominant intermediates. Disruption mutants show defects in growth on succinate. The UbiE polypeptide is required for the C methylation reactions in both ubiquinone and menaquinone biosynthesis
the dmtH gene is essential for bacteroid development and symbiotic nitrogen fixation ability. Menaquinone MK-7 is used as an electron carrier instead of ubiquinone in Mesorhizobium huakuii 7653R bacteroids. Menaquinone is synthesized only in bacteroids of wild type 7653R and not produced in free-living cells, the bacteroids use menaquinone as the specific electron carrier under symbiosis conditions to accommodate metabolic changes during development and nitrogen fixation
expression of MenG is essential. A gradual and partial depletion of MenG over consecutive subcultures results in progressive slowing of growth. Upon MenG depletion, there is a significant accumulation of MenG substrate, demethylmenaquinone, while the cellular level of menaquinone is unaffected. The growth retardation coincides with a lower oxygen consumption rate and ATP accumulation
the dmtH gene is essential for bacteroid development and symbiotic nitrogen fixation ability. Menaquinone MK-7 is used as an electron carrier instead of ubiquinone in Mesorhizobium huakuii 7653R bacteroids. Menaquinone is synthesized only in bacteroids of wild type 7653R and not produced in free-living cells, the bacteroids use menaquinone as the specific electron carrier under symbiosis conditions to accommodate metabolic changes during development and nitrogen fixation
mutant ubiE lacks ubiquinone due to defect in a specific methylation step of ubiquinone synthesis. Synthesis of menaquinone from demethylmenaquinone depends on the same gene ubiE. Mutant contains only demethylmenaquinone, but not menaquinone. Strain is able to grow with fumarate, trimethylamine N-oxide and dimethylsulfoxide, but not with nitrate as electron acceptor. Anaerobic respiration with fumarate and trimethylamine are catalyzed at 69% and 74% of wild-type rates, respectively. Dimethylsulfoxide respiration is reduced to 38% of wild-type, and nitrate respiration is below 8%
expression of MenG is essential. A gradual and partial depletion of MenG over consecutive subcultures results in progressive slowing of growth. Upon MenG depletion, there is a significant accumulation of MenG substrate, demethylmenaquinone, while the cellular level of menaquinone is unaffected. The growth retardation coincides with a lower oxygen consumption rate and ATP accumulation
mutant defective in the demthylmenaquinone methyltransferase activity expresses the same formate-dependent nitrite reduction activity as the parental strain
mutant ubiE lacks ubiquinone due to defect in a specific methylation step of ubiquinone synthesis. Synthesis of menaquinone from demethylmenaquinone depends on the same gene ubiE. Mutant contains only demethylmenaquinone, but not menaquinone. Strain is able to grow with fumarate, trimethylamine N-oxide and dimethylsulfoxide, but not with nitrate as electron acceptor. Anaerobic respiration with fumarate and trimethylamine are catalyzed at 69% and 74% of wild-type rates, respectively. Dimethylsulfoxide respiration is reduced to 38% of wild-type, and nitrate respiration is below 8%
mutant ubiE lacks ubiquinone due to defect in a specific methylation step of ubiquinone synthesis. Synthesis of menaquinone from demethylmenaquinone depends on the same gene ubiE. Mutant contains only demethylmenaquinone, but not menaquinone. Strain is able to grow with fumarate, trimethylamine N-oxide and dimethylsulfoxide, but not with nitrate as electron acceptor. Anaerobic respiration with fumarate and trimethylamine are catalyzed at 69% and 74% of wild-type rates, respectively. Dimethylsulfoxide respiration is reduced to 38% of wild-type, and nitrate respiration is below 8%
construction of dmtH null mutant strain HK116, symbiotic phenoytpe, HK116 forms small abnormal white nodules with a host plant, but lacks nitrogen-fixing ability. Nitrogenase activity of the nodules induced by HK116 is undetectable, and the number of nodules remarkably decreases compared to wild type 7653R
construction of dmtH null mutant strain HK116, symbiotic phenoytpe, HK116 forms small abnormal white nodules with a host plant, but lacks nitrogen-fixing ability. Nitrogenase activity of the nodules induced by HK116 is undetectable, and the number of nodules remarkably decreases compared to wild type 7653R
gene dmtH, cloned from a transposon-inserted mutant library, DNA and mino acid sequence determination and analysis. Real-time quantitative PCR shows that dmtH is a bacteroid-specific gene with highest expression at 25 days after inoculation of strain 7653R
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
gene expression is inducible by low temperatures, but not by high salt or dought. A T-DNA-tagged DMKT1 gene co-seggregates with the cold-inducible gus gene
An Escherichia coli mutant containing only demethylmenaquinone, but not menaquinone: effects on fumarate, dimethylsulfoxide, trimethylamine N-oxide and nitrate respiration
Identification of a novel gene cluster participating in menaquinone (vitamin K2) biosynthesis. Cloning and sequence determination of the 2-heptaprenyl-1,4-naphthoquinone methyltransferase gene of Bacillus stearothermophilus