We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
IUBMB Comments Requires Mg2+ . The enzyme, isolated from the bacterium Streptomyces fradiae , is involved in the biosynthesis of the macrolide antibiotic tylosin. The 2-hydroxy group of a 6-deoxy-D -allose moiety in demethylmacrocin acts as the methyl acceptor. Also acts on demethyllactenocin, giving lactenocin. Not identical with EC 2.1.1.101 macrocin O -methyltransferase.
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms demethylmacrocin o-methyltransferase, more
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
demethylmacrocin methyltransferase
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
S-adenosyl-L-methionine + demethylmacrocin = S-adenosyl-L-homocysteine + macrocin
the 2-hydroxyl group of a 6-deoxy-D-allose residue in demethylmacrocin acts as a methyl acceptor. Not identical with EC 2.1.101 macrocin O-methyltransferase
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
methyl group transfer
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
MetaCyc
tylosin biosynthesis
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
S-adenosyl-L-methionine:demethylmacrocin 2'''-O-methyltransferase
Requires Mg2+. The enzyme, isolated from the bacterium Streptomyces fradiae, is involved in the biosynthesis of the macrolide antibiotic tylosin. The 2-hydroxy group of a 6-deoxy-D-allose moiety in demethylmacrocin acts as the methyl acceptor. Also acts on demethyllactenocin, giving lactenocin. Not identical with EC 2.1.1.101 macrocin O-methyltransferase.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
S-adenosyl-L-methionine + demethyllactenocin
S-adenosyl-L-homocysteine + lactenocin
-
Substrates: - Products: -
?
S-adenosyl-L-methionine + demethylmacrocin
S-adenosyl-L-homocysteine + macrocin
S-adenosyl-L-methionine + demethylmacrocin
S-adenosyl-L-homocysteine + macrocin
-
Substrates: - Products: -
?
S-adenosyl-L-methionine + demethylmacrocin
S-adenosyl-L-homocysteine + macrocin
-
Substrates: enzyme catalyzes 2'''-O-methylation of bound 6-deoxy-D-allose Products: -
?
S-adenosyl-L-methionine + demethylmacrocin
S-adenosyl-L-homocysteine + macrocin
-
Substrates: penultimate step in tylosin biosynthesis Products: -
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
S-adenosyl-L-methionine + demethylmacrocin
S-adenosyl-L-homocysteine + macrocin
S-adenosyl-L-methionine + demethylmacrocin
S-adenosyl-L-homocysteine + macrocin
-
Substrates: enzyme catalyzes 2'''-O-methylation of bound 6-deoxy-D-allose Products: -
?
S-adenosyl-L-methionine + demethylmacrocin
S-adenosyl-L-homocysteine + macrocin
-
Substrates: penultimate step in tylosin biosynthesis Products: -
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Mg2+
-
required for maximal activity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
20-Dihydro-23-deoxymycaminosyltylonolide
-
-
20-dihydrolactenocin
-
inhibition of macrocin formation
23-demycinosyloxytylosin
-
-
23-Deoxy-O-mycaminosyltylonolide
-
-
lactenocin
-
inhibition of macrocin formation
macrocin
-
inhibition of lactenocin formation
mycaminosyltylonolide
-
-
O-Mycaminosyltylonolide
-
-
S-adenosyl-L-homocysteine
-
not D-enantiomer
sinefungin
-
competitive inhibition, poor inhibitor
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.01
demethyllactenocin
-
-
0.006
Demethylmacrocin
-
-
0.111
S-adenosylmethionine
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.186
23-demycinosyloxytylosin
-
-
0.073
23-Deoxy-O-mycaminosyltylonolide
-
-
0.065
mycaminosyltylonolide
-
-
0.11 - 0.226
S-adenosyl-L-homocysteine
0.057
A9145C
-
fixed substrate demethylmacrocin
0.108
A9145C
-
fixed substrate S-adenosylmethionine
0.022
lactenocin
-
fixed substrate demethylmacrocin
0.024
lactenocin
-
fixed substrate S-adenosylmethionine
0.037
macrocin
-
fixed substrate S-adenosylmethionine
0.067
macrocin
-
fixed substrate demethyllactenocin
0.11
S-adenosyl-L-homocysteine
-
fixed substrate demethylmacrocin
0.226
S-adenosyl-L-homocysteine
-
fixed substrate S-adenosylmethionine
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
-
brenda
Highest Expressing Human Cell Lines
Filter by:
Cell Line Links
Gene Links
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TYLE_STRFR
395
0
43233
Swiss-Prot
-
A0A5P9FBS9_9RHOB
237
0
27222
TrEMBL
-
A0A238JJ17_9RHOB
225
0
25496
TrEMBL
-
A0A177HHL2_9ACTN
282
0
31578
TrEMBL
-
A0A517LX93_9BACT
267
0
29974
TrEMBL
-
A0A120MY83_9SPHI
239
0
28262
TrEMBL
-
A0A1Y5RZ21_9RHOB
Roseovarius litorisediminis
544
0
61826
TrEMBL
-
A0A3N6DB02_9ACTN
403
0
44908
TrEMBL
-
A0A5P3AB09_9RHOB
235
0
26941
TrEMBL
-
A0A1V5ZQ58_9BACT
212
0
24600
TrEMBL
-
A0A0P1HBD3_9RHOB
237
0
26953
TrEMBL
-
A0A7W9HJY4_9PSEU
381
0
41658
TrEMBL
-
A0A143PY46_LUTPR
269
0
31771
TrEMBL
-
A0A0P0M9L0_9BURK
475
0
54067
TrEMBL
-
A0A1X6Z2B1_9RHOB
258
0
29206
TrEMBL
-
A0A2R8AET4_9RHOB
244
0
28060
TrEMBL
-
A0A840QJV9_9PSEU
416
0
46233
TrEMBL
-
A0A840Q8T4_9PSEU
393
0
43642
TrEMBL
-
A0A1D8BZ59_9PSEU
223
0
25045
TrEMBL
-
A0A1D8AVL6_9BACT
321
0
35226
TrEMBL
-
A0A517XGT3_9PLAN
903
0
103306
TrEMBL
-
A0A7W7T4W2_9PSEU
382
0
41442
TrEMBL
-
A0A6P2EA36_9BURK
1259
0
141260
TrEMBL
-
A0A149VYT9_9PROT
351
0
40522
TrEMBL
-
A0A5J4EAQ0_9BACT
235
0
26913
TrEMBL
-
A0A0P1G2D7_9RHOB
Tritonibacter multivorans
237
0
26557
TrEMBL
-
A0A7K0BU56_9ACTN
389
0
42951
TrEMBL
-
A0A1J5T8D0_9ZZZZ
262
0
29768
TrEMBL
other Location (Reliability: 4 )
A0A7M4DL11_9MICO
429
0
48512
TrEMBL
-
A0A517V633_9PLAN
276
0
31800
TrEMBL
-
A0A7Y9KT09_9ACTN
278
0
31646
TrEMBL
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
122000 - 126000
-
gel filtration
42000
-
3 * 42000, SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
trimer
-
3 * 42000, SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
4°C, quite stable in presence of S-adenosylmethionine and 10% ethanol, pH 7.0-8.0, stable for a minimum of 2 months
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pharmacology
-
tylosin fermentation, antibiotic biosynthesis
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Kreuzman, A.J.; Turner, J.R.; Yeh, W.K.
Two distinctive O-methyltransferases catalyzing penultimate and terminal reactions of macrolide antibiotic (tylosin) biosynthesis. Substrate specificity, enzyme inhibition, and kinetic mechanism
J. Biol. Chem.
263
15626-15633
1988
Streptomyces fradiae
brenda
html completed