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Information on EC 1.7.2.4 - nitrous-oxide reductase
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1.7.2.4 nitrous-oxide reductaseIUBMB Comments
The reaction is observed only in the direction of nitrous oxide reduction. Contains the mixed-valent dinuclear CuA species at the electron entry site of the enzyme, and the tetranuclear Cu-Z centre in the active site.
In Paracoccus pantotrophus, the electron donor is cytochrome c552.
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Word Map
- 1.7.2.4
-
denitrification
-
denitrify
- nitrite
-
greenhouse
- denitrificans
- paracoccus
- stutzeri
-
dinitrogen
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wastewater
-
binuclear
-
tetranuclear
- cycloclastes
-
mixed-valence
-
multicopper
-
ammonia-oxidizing
-
n-cycle
- nautica
-
wolinella
-
arable
- nitrospirae
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stratospheric
- agriculture
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t-rflp
- degradation
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Reaction Schemes
+
+
2
=
+
2
+
2
Synonyms
nitrous oxide reductase, n2o reductase, n(2)or, nitrous-oxide reductase, cnosz, hdn2or, psn2or, z-type n2or, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
cNosZ
EC 1.7.99.6
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-
formerly
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HdN2OR
MhN2OR
N(2)OR
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-
-
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N2O reductase
N2OR
nitrous oxide reductase
NosZ
Sden_2219
SdN2OR
N2O reductase
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-
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nitrous oxide reductase
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-
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-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
nitrogen + H2O + 2 ferricytochrome c = nitrous oxide + 2 ferrocytochrome c + 2 H+
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-
-
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nitrogen + H2O + 2 ferricytochrome c = nitrous oxide + 2 ferrocytochrome c + 2 H+
spectroscopic definition of the CuZ* intermediate in turnover of nitrous oxide reductase and molecular insight into the catalytic mechanism. The nature of N2O binding and reduction, specifies a molecular mechanism in which N2O coordinates in a mu-1,3 fashion to the fully reduced state, with hydrogen bonding from Lys397, and two electrons are transferred from the fully reduced mu4S2-bridged tetranuclear copper cluster to N2O via a single Cu atom to accomplish N-O bond cleavage
Marinobacter nauticus
nitrogen + H2O + 2 ferricytochrome c = nitrous oxide + 2 ferrocytochrome c + 2 H+
spectroscopic definition of the CuZ* intermediate in turnover of nitrous oxide reductase and molecular insight into the catalytic mechanism. The nature of N2O binding and reduction, specifies a molecular mechanism in which N2O coordinates in a mu-1,3 fashion to the fully reduced state, with hydrogen bonding from Lys397, and two electrons are transferred from the fully reduced mu4S2-bridged tetranuclear copper cluster to N2O via a single Cu atom to accomplish N-O bond cleavage
Marinobacter nauticus 617
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
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-
-
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redox reaction
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-
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reduction
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-
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-
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PATHWAY SOURCE
PATHWAYS
BRENDA
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SYSTEMATIC NAME
IUBMB Comments
nitrogen:cytochrome c oxidoreductase (N2O-forming)
The reaction is observed only in the direction of nitrous oxide reduction. Contains the mixed-valent dinuclear CuA species at the electron entry site of the enzyme, and the tetranuclear Cu-Z centre in the active site.
In Paracoccus pantotrophus, the electron donor is cytochrome c552.
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CAS REGISTRY NUMBER
COMMENTARY
55576-44-8
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
N2O + reduced benzyl viologen
N2 + oxidized benzyl viologen
-
-
-
?
N2O + reduced methyl viologen
N2 + H2O + oxidized methyl viologen
Marinobacter nauticus
-
-
-
-
?
N2O + reduced pseudoazurin
N2 + oxidized pseudoazurin
blue copper electron-transfer protein pseudoazurin is capable of mediating electron transfer to the nitrous oxide reductase. Pseudoazurin binds near copper site CuA, with parameters consistent with the formation of a transient, weakly-bound complex. Pseudoazurin a strong candidate for the physiological electron donor to th enzyme
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-
?
nitrogen + H2O + 2 ferricytochrome c
nitrous oxide + 2 ferrocytochrome c + 2 H+
Stutzerimonas stutzeri
-
-
-
?
nitrous oxide + benzyl viologen + H+
nitrogen + oxidized benzyl viologen + H2O
Stutzerimonas stutzeri
-
-
-
?
nitrous oxide + reduced benzyl viologen + H+
nitrogen + H2O + oxidized benzyl viologen
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-
-
-
r
nitrous oxide + reduced methyl viologen + H+
nitrogen + H2O + oxidized methyl viologen
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-
-
-
r
N2O + H2O + benzyl viologen cation radical
N2 + reduced benzyl viologen
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-
-
-
?
N2O + H2O + benzyl viologen cation radical
N2 + reduced benzyl viologen
-
-
-
-
?
N2O + reduced acceptor
N2 + H2O + acceptor
Pseudomonas denitrificans (nom. rej.) CCUG 2519
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-
-
-
?
N2O + reduced benzyl viologen
N2 + H2O + benzyl viologen
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-
-
-
?
N2O + reduced benzyl viologen
N2 + H2O + benzyl viologen
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-
-
-
?
N2O + reduced benzyl viologen
N2 + H2O + benzyl viologen
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-
-
-
?
N2O + reduced cytochrome c550
N2 + H2O + cytochrome c550
-
-
-
?
N2O + reduced methyl viologen
N2 + H2O + methyl viologen
-
-
-
-
?
N2O + reduced methyl viologen
N2 + oxidized methyl viologen
-
-
-
?
N2O + reduced methyl viologen
N2 + oxidized methyl viologen
-
-
-
?
nitrogen + H2O + acceptor
nitrous oxide + reduced acceptor
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-
-
?
nitrogen + H2O + acceptor
nitrous oxide + reduced acceptor
under some conditions, electron transfer may be rate limiting in N2O reduction. The transition state is stabilized by H-bonding interactions between the active site and an N2O-derived intermediate bound to the catalytic CuZ cluster
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-
?
nitrogen + H2O + acceptor
nitrous oxide + reduced acceptor
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-
-
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?
nitrous oxide + 2 ferrocytochrome c + 2 H+
nitrogen + H2O + 2 ferricytochrome c
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-
-
-
?
nitrous oxide + 2 ferrocytochrome c + 2 H+
nitrogen + H2O + 2 ferricytochrome c
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-
-
-
?
nitrous oxide + 2 ferrocytochrome c + 2 H+
nitrogen + H2O + 2 ferricytochrome c
Marinobacter nauticus
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-
-
-
?
nitrous oxide + 2 ferrocytochrome c + 2 H+
nitrogen + H2O + 2 ferricytochrome c
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-
-
?
nitrous oxide + 2 ferrocytochrome c + 2 H+
nitrogen + H2O + 2 ferricytochrome c
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-
-
?
nitrous oxide + 2 ferrocytochrome c + 2 H+
nitrogen + H2O + 2 ferricytochrome c
Stutzerimonas stutzeri
-
-
-
?
nitrous oxide + 2 ferrocytochrome c + 2 H+
nitrogen + H2O + 2 ferricytochrome c
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-
-
-
?
nitrous oxide + 2 ferrocytochrome c + 2 H+
nitrogen + H2O + 2 ferricytochrome c
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-
-
-
?
nitrous oxide + 2 ferrocytochrome c + 2 H+
nitrogen + H2O + 2 ferricytochrome c
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-
-
-
?
nitrous oxide + 2 reduced cytochrome c
nitrogen + H2O + 2 cytochrome c
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-
-
?
nitrous oxide + 2 reduced cytochrome c
nitrogen + H2O + 2 cytochrome c
-
-
-
?
nitrous oxide + 2 reduced cytochrome c
nitrogen + H2O + 2 cytochrome c
Marinobacter nauticus
-
-
-
?
nitrous oxide + 2 reduced cytochrome c
nitrogen + H2O + 2 cytochrome c
Marinobacter nauticus 617
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-
-
?
nitrous oxide + 2 reduced cytochrome c
nitrogen + H2O + 2 cytochrome c
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-
-
-
?
nitrous oxide + 2 reduced cytochrome c
nitrogen + H2O + 2 cytochrome c
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-
-
?
nitrous oxide + 2 reduced cytochrome c
nitrogen + H2O + 2 cytochrome c
the copper enzyme nitrous oxide reductase catalyzes the two-electron reduction of nitrous oxide (N2O) to dinitrogen
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?
nitrous oxide + 2 reduced cytochrome c
nitrogen + H2O + 2 cytochrome c
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-
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?
nitrous oxide + 2 reduced cytochrome c
nitrogen + H2O + 2 cytochrome c
the copper enzyme nitrous oxide reductase catalyzes the two-electron reduction of nitrous oxide (N2O) to dinitrogen
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?
nitrous oxide + 2 reduced cytochrome c
nitrogen + H2O + 2 cytochrome c
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-
-
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?
nitrous oxide + 2 reduced cytochrome c
nitrogen + H2O + 2 cytochrome c
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-
-
-
?
nitrous oxide + 2 reduced cytochrome c
nitrogen + H2O + 2 cytochrome c
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-
-
?
nitrous oxide + 2 reduced methyl viologen
nitrogen + H2O + 2 methyl viologen
Marinobacter nauticus
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-
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?
nitrous oxide + 2 reduced methyl viologen
nitrogen + H2O + 2 methyl viologen
Marinobacter nauticus 617
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?
nitrous oxide + reduced acceptor
nitrogen + H2O + acceptor
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?
nitrous oxide + reduced acceptor
nitrogen + H2O + acceptor
-
methyl viologen as electron donor
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?
nitrous oxide + reduced acceptor
nitrogen + H2O + acceptor
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?
nitrous oxide + reduced acceptor
nitrogen + H2O + acceptor
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dithionite-reduced methyl viologen as electron donor
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?
nitrous oxide + reduced acceptor
nitrogen + H2O + acceptor
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?
nitrous oxide + reduced acceptor
nitrogen + H2O + acceptor
-
photochemically-reduced benzyl viologen as electron donor
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?
nitrous oxide + reduced acceptor
nitrogen + H2O + acceptor
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photochemically reduced benzyl viologen and 3,3',5,5'-tetramethylbenzidine as electron donor
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?
nitrous oxide + reduced acceptor
nitrogen + H2O + acceptor
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dithionite-reduced benzyl viologen as electron donor
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?
nitrous oxide + reduced acceptor
nitrogen + H2O + acceptor
Marinobacter nauticus
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-
-
-
?
nitrous oxide + reduced acceptor
nitrogen + H2O + acceptor
Marinobacter nauticus 617
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-
-
-
?
nitrous oxide + reduced acceptor
nitrogen + H2O + acceptor
-
-
-
-
?
nitrous oxide + reduced acceptor
nitrogen + H2O + acceptor
-
methyl viologen as electron donor
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?
nitrous oxide + reduced acceptor
nitrogen + H2O + acceptor
-
photochemically-reduced benzyl viologen as electron donor
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?
nitrous oxide + reduced acceptor
nitrogen + H2O + acceptor
-
a mixture of N,N,N',N'-tetramethyl-p-phenylenediamine and ascorbate as electron donor
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?
nitrous oxide + reduced acceptor
nitrogen + H2O + acceptor
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-
-
-
?
nitrous oxide + reduced acceptor
nitrogen + H2O + acceptor
-
methyl viologen as electron donor, activity after incubation with ferricyanide, ascorbate or dithionite
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-
?
nitrous oxide + reduced acceptor
nitrogen + H2O + acceptor
-
dithionite-reduced methyl viologen as electron donor
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-
?
nitrous oxide + reduced acceptor
nitrogen + H2O + acceptor
-
-
-
-
?
nitrous oxide + reduced acceptor
nitrogen + H2O + acceptor
-
dithionite-reduced methyl viologen as electron donor
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-
?
nitrous oxide + reduced acceptor
nitrogen + H2O + acceptor
-
-
-
-
?
nitrous oxide + reduced acceptor
nitrogen + H2O + acceptor
-
photochemically-reduced benzyl viologen as electron donor
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-
?
nitrous oxide + reduced acceptor
nitrogen + H2O + acceptor
-
-
-
-
?
nitrous oxide + reduced acceptor
nitrogen + H2O + acceptor
-
methyl viologen as electron donor
-
-
?
nitrous oxide + reduced acceptor
nitrogen + H2O + acceptor
-
photochemically-reduced benzyl viologen as electron donor
-
-
?
nitrous oxide + reduced acceptor
nitrogen + H2O + acceptor
-
-
-
-
?
nitrous oxide + reduced acceptor
nitrogen + H2O + acceptor
-
-
-
-
?
nitrous oxide + reduced acceptor
nitrogen + H2O + acceptor
-
-
-
-
?
nitrous oxide + reduced acceptor
nitrogen + H2O + acceptor
-
-
-
-
?
nitrous oxide + reduced acceptor
nitrogen + H2O + acceptor
-
-
-
-
?
nitrous oxide + reduced acceptor
nitrogen + H2O + acceptor
-
photochemically-reduced benzyl viologen as electron donor
-
-
?
nitrous oxide + reduced methyl viologen
nitrogen + H2O + methyl viologen
-
-
-
?
nitrous oxide + reduced methyl viologen
nitrogen + H2O + methyl viologen
-
-
-
?
additional information
?
-
Marinobacter nauticus
-
the purple form of enzyme, in which the copper centre is in the oxidized [2Cu2+:2Cu+] redox state, is redox active, although it is still catalytically non-competent, as its specific activity is lower than that of the activated fully reduced enzyme and comparable with that of the enzyme with the copper centre in either the [1Cu2+:3Cu+] redox state or in the redox inactive state
-
-
?
additional information
?
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-
determination of detailed gas kinetics and transcription patterns from batch culture experiments with Paracoccus denitrificans, allowing in vivo estimation of electron flow to O2 and N2O under various O2 regimes
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?
additional information
?
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-
enzyme shows Cu+/Fe2+ oxidation kinetics that follow the Michaelis-Menten model, with 2fold to 10fold higher efficiencies for Cu+ and Fe2+ as compared with the tested aromatic compounds
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
N2O + reduced pseudoazurin
N2 + oxidized pseudoazurin
blue copper electron-transfer protein pseudoazurin is capable of mediating electron transfer to the nitrous oxide reductase. Pseudoazurin binds near copper site CuA, with parameters consistent with the formation of a transient, weakly-bound complex. Pseudoazurin a strong candidate for the physiological electron donor to th enzyme
-
-
?
nitrogen + H2O + 2 ferricytochrome c
nitrous oxide + 2 ferrocytochrome c + 2 H+
Stutzerimonas stutzeri
-
-
-
?
nitrogen + H2O + acceptor
nitrous oxide + reduced acceptor
-
-
-
?