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3,4-dehydro-L-proline + acceptor + H2O
? + reduced acceptor
efficient substrate
-
-
?
4-methylene-L-proline + acceptor + H2O
DELTA1-pyrroline-3-methylene-5-carboxylic acid + reduced acceptor
-
-
-
?
glycine + 2,6-dichlorophenolindophenol + H2O
? + reduced 2,6-dichlorophenolindophenol
L-alanine + 2,6-dichlorophenolindophenol + H2O
? + reduced 2,6-dichlorophenolindophenol
L-alanine + 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium + H2O
?
L-arginine + 2,6-dichlorophenolindophenol + H2O
? + reduced 2,6-dichlorophenolindophenol
L-histidine + 2,6-dichlorophenolindophenol + H2O
? + reduced 2,6-dichlorophenolindophenol
L-hydroxyproline + 2,6-dichlorophenolindophenol + H2O
? + reduced 2,6-dichlorophenolindophenol
L-hydroxyproline + 2,6-dichlorophenolindophenol + H2O
DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
100% activity compared to L-proline
-
-
?
L-hydroxyproline + oxidized 2,6-dichlorophenolindophenol + H2O
?
-
reaction rate is 39% of that with L-proline
-
-
?
L-leucine + 2,6-dichlorophenolindophenol + H2O
? + reduced 2,6-dichlorophenolindophenol
-
42% activity compared to L-proline
-
-
?
L-pipecolic acid + acceptor + H2O
? + reduced acceptor
-
-
-
-
?
L-proline + 2,6-dichlorophenolindophenol
(S)-1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
L-proline + 2,6-dichlorophenolindophenol + H2O
(S)-1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
L-proline + 2,6-dichlorophenolindophenol + H2O
DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
L-proline + 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium
DELTA1-pyrroline-5-carboxylate + reduced 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium
-
-
-
-
?
L-proline + 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium + H2O
DELTA1-pyrroline-5-carboxylate + reduced 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium
L-proline + 4-iodonitrotetrazolium violet + H2O
DELTA1-pyrroline-5-carboxylate + reduced 4-iodonitrotetrazolium violet
L-proline + acceptor
(S)-1-pyrroline-5-carboxylate + reduced acceptor
L-proline + acceptor + H2O
(S)-1-pyrroline-5-carboxylate + reduced acceptor
L-proline + coenzyme Q1 + H2O
DELTA1-pyrroline-5-carboxylate + reduced coenzyme Q1
-
-
-
?
L-proline + cytochrome c + H2O
(S)-1-pyrroline-5-carboxylate + reduced cytochrome c
L-proline + FAD
(S)-1-pyrroline-5-carboxylate + FADH2
L-proline + ferricyanide
DELTA1-pyrroline-5-carboxylate + ferrocyanide
L-proline + H2O
(S)-1-pyrroline-5-carboxylate + H2O2
-
-
-
?
L-proline + menadione
(S)-1-pyrroline-5-carboxylate + menadiol
-
-
-
-
r
L-proline + oxidized 2,6-dichlorophenolindophenol + H2O
(S)-1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
the enzyme does not utilize ferricyanide or 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide as the electron acceptor, activity with phenazine methosulfate-p-iodonitrotetrazolium violet is 10% of that with 2,6-dichlorophenolindophenol
-
-
?
L-proline + oxidized dichlorophenolindophenol
1-pyrroline-5-carboxylate + reduced dichlorophenolindophenol
L-proline + oxidized ferrocenecarboxylic acid
1-pyrroline-5-carboxylate + reduced ferrocenecarboxylic acid
L-proline + oxidized phenazine methosulfate
(S)-1-pyrroline-5-carboxylate + reduced phenazine methosulfate
-
-
-
-
?
L-proline + p-iodonitrotetrazolium + H2O
(S)-1-pyrroline-5-carboxylate + reduced p-iodonitrotetrazolium
-
-
-
?
L-serine + 2,6-dichlorophenolindophenol + H2O
? + reduced 2,6-dichlorophenolindophenol
-
55% activity compared to L-proline
-
-
?
L-threonine + 2,6-dichlorophenolindophenol + H2O
? + reduced 2,6-dichlorophenolindophenol
-
66% activity compared to L-proline
-
-
?
L-threonine + 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium + H2O
?
L-valine + 2,6-dichlorophenolindophenol + H2O
? + reduced 2,6-dichlorophenolindophenol
-
33% activity compared to L-proline
-
-
?
sarcosine + acceptor + H2O
? + reduced acceptor
-
-
-
-
?
additional information
?
-
glycine + 2,6-dichlorophenolindophenol + H2O
? + reduced 2,6-dichlorophenolindophenol
-
52% activity compared to L-proline
-
-
?
glycine + 2,6-dichlorophenolindophenol + H2O
? + reduced 2,6-dichlorophenolindophenol
-
52% activity compared to L-proline
-
-
?
L-alanine + 2,6-dichlorophenolindophenol + H2O
? + reduced 2,6-dichlorophenolindophenol
-
48% activity compared to L-proline
-
-
?
L-alanine + 2,6-dichlorophenolindophenol + H2O
? + reduced 2,6-dichlorophenolindophenol
-
48% activity compared to L-proline
-
-
?
L-alanine + 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium + H2O
?
-
weak activity
-
-
?
L-alanine + 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium + H2O
?
-
weak activity
-
-
?
L-arginine + 2,6-dichlorophenolindophenol + H2O
? + reduced 2,6-dichlorophenolindophenol
-
62% activity compared to L-proline
-
-
?
L-arginine + 2,6-dichlorophenolindophenol + H2O
? + reduced 2,6-dichlorophenolindophenol
-
62% activity compared to L-proline
-
-
?
L-histidine + 2,6-dichlorophenolindophenol + H2O
? + reduced 2,6-dichlorophenolindophenol
-
72% activity compared to L-proline
-
-
?
L-histidine + 2,6-dichlorophenolindophenol + H2O
? + reduced 2,6-dichlorophenolindophenol
-
72% activity compared to L-proline
-
-
?
L-hydroxyproline + 2,6-dichlorophenolindophenol + H2O
? + reduced 2,6-dichlorophenolindophenol
-
72% activity compared to L-proline
-
-
?
L-hydroxyproline + 2,6-dichlorophenolindophenol + H2O
? + reduced 2,6-dichlorophenolindophenol
-
72% activity compared to L-proline
-
-
?
L-proline + 2,6-dichlorophenolindophenol
(S)-1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
artificial electron acceptor
-
-
?
L-proline + 2,6-dichlorophenolindophenol
(S)-1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
artificial electron acceptor
-
-
?
L-proline + 2,6-dichlorophenolindophenol
(S)-1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
artificial electron acceptor
-
-
?
L-proline + 2,6-dichlorophenolindophenol + H2O
(S)-1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
L-proline + 2,6-dichlorophenolindophenol + H2O
(S)-1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
L-proline + 2,6-dichlorophenolindophenol + H2O
(S)-1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
L-proline + 2,6-dichlorophenolindophenol + H2O
DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
L-proline + 2,6-dichlorophenolindophenol + H2O
DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
L-proline + 2,6-dichlorophenolindophenol + H2O
DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
100% activity
-
-
?
L-proline + 2,6-dichlorophenolindophenol + H2O
DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
L-proline is the most preferred substrate and 2,6-dichlorophenolindophenol is the most preferred electron acceptor
-
-
?
L-proline + 2,6-dichlorophenolindophenol + H2O
DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
L-proline is the most preferred substrate and 2,6-dichlorophenolindophenol is the most preferred electron acceptor
-
-
?
L-proline + 2,6-dichlorophenolindophenol + H2O
DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
the alphabetagammadelta-type beta subunit of L-proline dehydrogenase prefers 2, 6-dichloroindophenol as electron acceptor
-
-
?
L-proline + 2,6-dichlorophenolindophenol + H2O
DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
the alphabetagammadelta-type beta subunit of L-proline dehydrogenase prefers 2, 6-dichloroindophenol as electron acceptor
-
-
?
L-proline + 2,6-dichlorophenolindophenol + H2O
DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
the alphabetagammadelta-type beta subunit of L-proline dehydrogenase prefers 2, 6-dichloroindophenol as electron acceptor
-
-
?
L-proline + 2,6-dichlorophenolindophenol + H2O
DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
the alphabetagammadelta-type beta subunit of L-proline dehydrogenase prefers 2, 6-dichloroindophenol as electron acceptor
-
-
?
L-proline + 2,6-dichlorophenolindophenol + H2O
DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
-
the alphabetagammadelta-type beta subunit of L-proline dehydrogenase prefers 2, 6-dichloroindophenol as electron acceptor
-
-
?
L-proline + 2,6-dichlorophenolindophenol + H2O
DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
the alphabetagammadelta-type beta subunit of L-proline dehydrogenase prefers 2, 6-dichloroindophenol as electron acceptor
-
-
?
L-proline + 2,6-dichlorophenolindophenol + H2O
DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
the alphabetagammadelta-type beta subunit of L-proline dehydrogenase prefers 2, 6-dichloroindophenol as electron acceptor
-
-
?
L-proline + 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium + H2O
DELTA1-pyrroline-5-carboxylate + reduced 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium
-
-
-
-
?
L-proline + 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium + H2O
DELTA1-pyrroline-5-carboxylate + reduced 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium
-
-
-
-
?
L-proline + 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium + H2O
DELTA1-pyrroline-5-carboxylate + reduced 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium
-
-
-
-
?
L-proline + 4-iodonitrotetrazolium violet + H2O
DELTA1-pyrroline-5-carboxylate + reduced 4-iodonitrotetrazolium violet
-
9% activity compared to 2,6-dichlorophenolindophenol
-
-
?
L-proline + 4-iodonitrotetrazolium violet + H2O
DELTA1-pyrroline-5-carboxylate + reduced 4-iodonitrotetrazolium violet
-
9% activity compared to 2,6-dichlorophenolindophenol
-
-
?
L-proline + 4-iodonitrotetrazolium violet + H2O
DELTA1-pyrroline-5-carboxylate + reduced 4-iodonitrotetrazolium violet
-
-
-
-
?
L-proline + 4-iodonitrotetrazolium violet + H2O
DELTA1-pyrroline-5-carboxylate + reduced 4-iodonitrotetrazolium violet
-
-
-
-
?
L-proline + 4-iodonitrotetrazolium violet + H2O
DELTA1-pyrroline-5-carboxylate + reduced 4-iodonitrotetrazolium violet
-
-
-
-
?
L-proline + 4-iodonitrotetrazolium violet + H2O
DELTA1-pyrroline-5-carboxylate + reduced 4-iodonitrotetrazolium violet
-
-
-
-
?
L-proline + 4-iodonitrotetrazolium violet + H2O
DELTA1-pyrroline-5-carboxylate + reduced 4-iodonitrotetrazolium violet
-
-
-
-
?
L-proline + 4-iodonitrotetrazolium violet + H2O
DELTA1-pyrroline-5-carboxylate + reduced 4-iodonitrotetrazolium violet
-
-
-
?
L-proline + 4-iodonitrotetrazolium violet + H2O
DELTA1-pyrroline-5-carboxylate + reduced 4-iodonitrotetrazolium violet
-
-
-
?
L-proline + acceptor
(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
-
-
-
?
L-proline + acceptor
(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
-
-
?
L-proline + acceptor
(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
-
-
?
L-proline + acceptor
(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
-
-
?
L-proline + acceptor + H2O
(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
-
-
-
?
L-proline + acceptor + H2O
(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
-
-
-
?
L-proline + acceptor + H2O
(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
-
-
-
?
L-proline + acceptor + H2O
(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
-
-
-
?
L-proline + acceptor + H2O
(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
-
-
ir
L-proline + acceptor + H2O
(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
-
-
?
L-proline + acceptor + H2O
(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate, ferricyanide, menadione, cytochrome c
-
?
L-proline + acceptor + H2O
(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
-
-
?
L-proline + acceptor + H2O
(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate, ferricyanide, menadione, cytochrome c
-
?
L-proline + acceptor + H2O
(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
-
-
?
L-proline + acceptor + H2O
(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
-
-
-
?
L-proline + acceptor + H2O
(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
-
-
?
L-proline + acceptor + H2O
(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate, ferricyanide, menadione, cytochrome c
-
?
L-proline + acceptor + H2O
(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
highly specific for L-form of proline
-
?
L-proline + acceptor + H2O
(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
preferred substrate
-
-
?
L-proline + acceptor + H2O
(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
-
-
?
L-proline + acceptor + H2O
(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
-
-
-
?
L-proline + acceptor + H2O
(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate, ferricyanide, menadione, cytochrome c
-
?
L-proline + acceptor + H2O
(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
-
-
?
L-proline + acceptor + H2O
(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate, ferricyanide, menadione, cytochrome c
-
?
L-proline + acceptor + H2O
(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
-
-
?
L-proline + acceptor + H2O
(S)-1-pyrroline-5-carboxylate + reduced acceptor
-
acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate, ferricyanide, menadione, cytochrome c
-
?
L-proline + cytochrome c + H2O
(S)-1-pyrroline-5-carboxylate + reduced cytochrome c
-
-
-
-
?
L-proline + cytochrome c + H2O
(S)-1-pyrroline-5-carboxylate + reduced cytochrome c
-
-
-
?
L-proline + FAD
(S)-1-pyrroline-5-carboxylate + FADH2
-
-
-
?
L-proline + FAD
(S)-1-pyrroline-5-carboxylate + FADH2
-
-
-
-
r
L-proline + ferricyanide
DELTA1-pyrroline-5-carboxylate + ferrocyanide
-
93% activity compared to 2,6-dichlorophenolindophenol
-
-
?
L-proline + ferricyanide
DELTA1-pyrroline-5-carboxylate + ferrocyanide
-
93% activity compared to 2,6-dichlorophenolindophenol
-
-
?
L-proline + ferricyanide
DELTA1-pyrroline-5-carboxylate + ferrocyanide
-
the alpha4beta4-type beta subunit of L-proline dehydrogenase utilizes ferricyanide as the most preferable electron acceptor
-
-
?
L-proline + ferricyanide
DELTA1-pyrroline-5-carboxylate + ferrocyanide
-
the alpha4beta4-type beta subunit of L-proline dehydrogenase utilizes ferricyanide as the most preferable electron acceptor
-
-
?
L-proline + ferricyanide
DELTA1-pyrroline-5-carboxylate + ferrocyanide
-
the alpha4beta4-type beta subunit of L-proline dehydrogenase utilizes ferricyanide as the most preferable electron acceptor
-
-
?
L-proline + ferricyanide
DELTA1-pyrroline-5-carboxylate + ferrocyanide
-
the alpha4beta4-type beta subunit of L-proline dehydrogenase utilizes ferricyanide as the most preferable electron acceptor
-
-
?
L-proline + ferricyanide
DELTA1-pyrroline-5-carboxylate + ferrocyanide
-
the alpha4beta4-type beta subunit of L-proline dehydrogenase utilizes ferricyanide as the most preferable electron acceptor
-
-
?
L-proline + ferricyanide
DELTA1-pyrroline-5-carboxylate + ferrocyanide
the alpha4beta4-type beta subunit of L-proline dehydrogenase utilizes ferricyanide as the most preferable electron acceptor
-
-
?
L-proline + ferricyanide
DELTA1-pyrroline-5-carboxylate + ferrocyanide
the alpha4beta4-type beta subunit of L-proline dehydrogenase utilizes ferricyanide as the most preferable electron acceptor
-
-
?
L-proline + oxidized dichlorophenolindophenol
1-pyrroline-5-carboxylate + reduced dichlorophenolindophenol
-
-
-
?
L-proline + oxidized dichlorophenolindophenol
1-pyrroline-5-carboxylate + reduced dichlorophenolindophenol
-
-
-
?
L-proline + oxidized ferrocenecarboxylic acid
1-pyrroline-5-carboxylate + reduced ferrocenecarboxylic acid
-
-
-
?
L-proline + oxidized ferrocenecarboxylic acid
1-pyrroline-5-carboxylate + reduced ferrocenecarboxylic acid
-
-
-
?
L-threonine + 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium + H2O
?
-
weak activity
-
-
?
L-threonine + 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium + H2O
?
-
weak activity
-
-
?
additional information
?
-
-
no activity with: D-proline, glycine, L-glutamate, L-aspartate, L-arginine, L-lysine, L-serine, L-leucine, L-alanine, L-threonine, L-methionine,L-tryptophan, L-tyrosine
-
-
?
additional information
?
-
-
no activity with D-proline, L-tryptophan, L-glutamate, and L-aspartate
-
-
?
additional information
?
-
-
no activity with D-proline, L-tryptophan, L-glutamate, and L-aspartate
-
-
?
additional information
?
-
-
D-proline, L-hydroxyproline, L-arginine, L-aspartate, and glycine are inert as substrates
-
-
?
additional information
?
-
-
D-proline, L-hydroxyproline, L-arginine, L-aspartate, and glycine are inert as substrates
-
-
?
additional information
?
-
-
sarcosine, D-proline, L-ornithine, L-glutamate, L-arginine, L-serine, L-leucine, L-valine, and L-alanine are inert as substrates. NAD+, NADP+, and bovine heart cytochrome c are inert as electron acceptors
-
-
?
additional information
?
-
-
sarcosine, D-proline, L-ornithine, L-glutamate, L-arginine, L-serine, L-leucine, L-valine, and L-alanine are inert as substrates. NAD+, NADP+, and bovine heart cytochrome c are inert as electron acceptors
-
-
?
additional information
?
-
-
does not reduce NAD+
-
-
?
additional information
?
-
-
D-proline, L-hydroxyproline, pyrrolidone-5-carboxylate, sarcosine, and glycine are inert as substrates
-
-
?
additional information
?
-
-
isoform PDH1, no substrate: L-hydroxyproline, D-proline, cis-4-hydroxy-D-proline, L-2-pyrrolidone-5-carboxylate, pyrrole-5-carboxylate, pipecolic acid
-
-
?
additional information
?
-
-
D-proline, L-hydroxyproline, pyrrolidone-5-carboxylate, sarcosine, and glycine are inert as substrates
-
-
?
additional information
?
-
-
D-proline, L-hydroxyproline, pyrrolidone-5-carboxylate, sarcosine, and glycine are inert as substrates
-
-
?
additional information
?
-
-
isoform PDH1, no substrate: L-hydroxyproline, D-proline, cis-4-hydroxy-D-proline, L-2-pyrrolidone-5-carboxylate, pyrrole-5-carboxylate, pipecolic acid
-
-
?
additional information
?
-
-
D-proline, L-hydroxyproline, pyrrolidone-5-carboxylate, sarcosine, and glycine are inert as substrates
-
-
?
additional information
?
-
-
D-proline, L-hydroxyproline, pyrrolidone-5-carboxylate, sarcosine, and glycine are inert as substrates
-
-
?
additional information
?
-
D-proline, L-hydroxyproline, pyrrolidone-5-carboxylate, sarcosine, and glycine are inert as substrates
-
-
?
additional information
?
-
D-proline, L-hydroxyproline, pyrrolidone-5-carboxylate, sarcosine, and glycine are inert as substrates
-
-
?
additional information
?
-
trans-4-hydroxy-L-proline, cis-4-hydroxy-L-proline, L-azetidine-2-carboxylic acid, and L-pipecolinic acid are no substrates of PRODH
-
-
?
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0.026 - 3.4
2,6-dichlorophenolindophenol
4
3,4-dehydro-L-proline
in 50 mM Tris-HCl, at 25°C
0.028 - 0.155
coenzyme Q1
6.05
L-hydroxyproline
-
in 200 mM Tris-HCl buffer (pH 8.0), at 50°C
212.3
L-pipecolic acid
-
wild type enzyme, in 100 mM potassium phosphate buffer, pH 7.5, at 80°C
0.14
oxidized 2,6-dichlorophenolindophenol
-
pH 6.5, 50°C
additional information
additional information
-
0.026
2,6-dichlorophenolindophenol
-
with L-proline as cosubstrate, in 200 mM Tris-HCl buffer (pH 8.0), at 50°C
0.03
2,6-dichlorophenolindophenol
-
isoform PDH1
0.035
2,6-dichlorophenolindophenol
-
with L-hydroxyproline as cosubstrate, in 200 mM Tris-HCl buffer (pH 8.0), at 50°C
3.4
2,6-dichlorophenolindophenol
pH 7.1, 25°C, recombinant wild-type enzyme
0.028
coenzyme Q1
mutant enzyme G63A, at 23°C, in 50 mM potassium phosphate and 25 mM NaCl (pH 7.5)
0.032
coenzyme Q1
mutant enzyme E64A, at 23°C, in 50 mM potassium phosphate and 25 mM NaCl (pH 7.5)
0.155
coenzyme Q1
wild type enzyme, at 23°C, in 50 mM potassium phosphate and 25 mM NaCl (pH 7.5)
0.174
L-proline
-
PF1798 (alphabetagammadelta-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C
0.28
L-proline
-
pH 6.5, 50°C
0.28
L-proline
-
wild type enzyme, at pH 8.0 and 50°C
0.334
L-proline
-
PH1751 (alphabetagammadelta-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C
0.47
L-proline
-
in 10 mM MOPS, pH 7.5, at 25°C
0.689
L-proline
-
PF1246 (alpha4beta4-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C
1.46
L-proline
-
TK0117 (alpha4beta4-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C
1.67
L-proline
-
in 200 mM Tris-HCl buffer (pH 8.0), at 50°C
1.95
L-proline
-
mutant enzyme Y215F, in 100 mM potassium phosphate buffer, pH 7.5, at 60°C
2 - 6
L-proline
-
in presence of ADP
2 - 6
L-proline
-
depends on assay method
2.38
L-proline
-
TK0122 (alphabetagammadelta-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C
2.46
L-proline
TPpdhB (alphabetagammadelta-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C
2.5 - 5
L-proline
-
PF1364 (alpha4beta4-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C
3.5
L-proline
TPpdhB2 (alpha4beta4-type beta subunit of L-proline dehydrogenase) in 200 mM Tris-HCl (pH 8.0), at 50°C
4
L-proline
-
isoform PDH1
5.3
L-proline
-
wild type enzyme, in 100 mM potassium phosphate buffer, pH 7.5, at 50°C
5.6
L-proline
-
wild type enzyme, in 100 mM potassium phosphate buffer, pH 7.5, at 40°C
5.7
L-proline
pH 7.1, 25°C, recombinant wild-type enzyme
6.5
L-proline
pH 7.4, 45°C, recombinant wild-type enzyme, Haldane kinetics
9.9
L-proline
-
wild type enzyme, in 100 mM potassium phosphate buffer, pH 7.5, at 60°C
11.5
L-proline
pH 7.4, 25°C, recombinant wild-type enzyme, Michaelis-Menten kinetics
13
L-proline
with O2 as cosubstrate, in 50 mM Tris-HCl, at 25°C
14.46
L-proline
-
mutant enzyme H225Q, in 100 mM potassium phosphate buffer, pH 7.5, at 60°C
16.5
L-proline
pH 7.4, 25°C, recombinant wild-type enzyme, Haldane kinetics
19.67
L-proline
-
mutant enzyme H225A, in 100 mM potassium phosphate buffer, pH 7.5, at 60°C
19.9
L-proline
-
wild type enzyme, in 100 mM potassium phosphate buffer, pH 7.5, at 70°C
27
L-proline
with 2,6-dichlorophenolindophenol as cosubstrate, in 50 mM Tris-HCl, at 25°C
30.8
L-proline
-
wild type enzyme, in 100 mM potassium phosphate buffer, pH 7.5, at 80°C
31
L-proline
-
in 100 mM TrisHCl, 10 mM MgCl2, 10% (v/v) glycerol, pH 8.5, at 25°C
33
L-proline
-
without addition of ADP
33.8
L-proline
pH 7.4, 45°C, recombinant mutant F10E/L12E, Haldane kinetics
35
L-proline
-
at pH 8.5 and 30°C
50
L-proline
mutant enzyme E64A, at 23°C, in 50 mM potassium phosphate and 25 mM NaCl (pH 7.5)
67.6
L-proline
pH 7.4, 25°C, recombinant mutant F10E/L12E, Michaelis-Menten kinetics
175.1
L-proline
pH 7.4, 25°C, recombinant mutant F10E/L12E, Haldane kinetics
230
L-proline
-
DELTAL428 mutant enzyme, at pH 8.0 and 50°C
290
L-proline
wild type enzyme, at 23°C, in 50 mM potassium phosphate and 25 mM NaCl (pH 7.5)
384
L-proline
mutant enzyme G63A, at 23°C, in 50 mM potassium phosphate and 25 mM NaCl (pH 7.5)
860
L-proline
pH 7.1, 25°C, recombinant mutant Y203F
additional information
additional information
Michaelis-Menten and Haldane kinetics
-
additional information
additional information
Michaelis-Menten kinetics, steady-state kinetics and pre-steady-state kinetics of wild-type and mutant enzymes, stopped-flow kinetic isotope effects, bisubstrate kinetic analysis, overview
-
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Meile, L.; Leisinger, T.
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1982
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brenda
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42
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2006
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-
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369
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2008
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55
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Homo sapiens
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18
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43
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-
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89
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Process integration for the recovery and purification of recombinant Pseudomonas fluorescens proline dehydrogenase using aqueous two-phase systems
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929
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Pseudomonas fluorescens
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Proline dehydrogenase contributes to pathogen defense in Arabidopsis
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Tallarita, E.; Pollegioni, L.; Servi, S.; Molla, G.
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Homo sapiens
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Satomura, T.; Hara, Y.; Suye, S.; Sakuraba, H.; Ohshima, T.
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Aeropyrum pernix
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Mycobacterium tuberculosis (O50444), Mycobacterium tuberculosis H37Rv (O50444)
brenda
Monteoliva, M.I.; Rizzi, Y.S.; Cecchini, N.M.; Hajirezaei, M.R.; Alvarez, M.E.
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21
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Mohammadi, H.S.; Omidinia, E.
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929
11-17
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Huijbers, M.; van Berkel, W.
A more polar N-terminal helix releases MBP-tagged Thermus thermophilus proline dehydrogenase from tetramer-polymer self-association
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134
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Thermus thermophilus (Q72IB8)
-
brenda
Tonooka, A.; Komatsu, T.; Tanaka, S.; Sakamoto, H.; Satomura, T.; Suye, S.I.
A L-proline/O2 biofuel cell using L-proline dehydrogenase (LPDH) from Aeropyrum pernix
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45
1821-1825
2018
Aeropyrum pernix (Q9YCJ0), Aeropyrum pernix DSM 11879 (Q9YCJ0)
brenda