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IUBMB Comments The enzyme from Pseudomonas sp. AK2 also acts on quinoline-8-carboxylate, whereas that from Serratia marcescens 2CC-1 will oxidize nicotinate; quinaldate is a substrate for both of these enzymes. 2,4,6-Trinitrobenzene sulfonate, 1,4-benzoquinone, 1,2-naphthoquinone, nitroblue tetrazolium, thionine and menadione will serve as an electron acceptor for the former enzyme and ferricyanide for the latter; Meldola blue, iodonitrotetrazolium chloride, phenazine methosulfate, 2,6-dichlorophenolindophenol and cytochrome c will act as electron acceptors for both.
The enzyme appears in viruses and cellular organisms
Synonyms
quinaldic acid 4-oxidoreductase,
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Oxidase, quinaldate 4-
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Quinaldic acid 4-oxidoreductase
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Quinaldate + acceptor + H2O = kynurenate + reduced acceptor
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quinoline-2-carboxylate:acceptor 4-oxidoreductase (hydroxylating)
The enzyme from Pseudomonas sp. AK2 also acts on quinoline-8-carboxylate, whereas that from Serratia marcescens 2CC-1 will oxidize nicotinate; quinaldate is a substrate for both of these enzymes. 2,4,6-Trinitrobenzene sulfonate, 1,4-benzoquinone, 1,2-naphthoquinone, nitroblue tetrazolium, thionine and menadione will serve as an electron acceptor for the former enzyme and ferricyanide for the latter; Meldola blue, iodonitrotetrazolium chloride, phenazine methosulfate, 2,6-dichlorophenolindophenol and cytochrome c will act as electron acceptors for both.
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nicotinic acid + acceptor
6-hydroxynicotinic acid + reduced acceptor
quinaldate + 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride + H2O
kynurenate + reduced 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride
quinaldate + acceptor
kynurenate + reduced acceptor
quinoline 8-carboxylic acid + reduced acceptor
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nicotinic acid + acceptor
6-hydroxynicotinic acid + reduced acceptor
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nicotinic acid + acceptor
6-hydroxynicotinic acid + reduced acceptor
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quinaldate + 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride + H2O
kynurenate + reduced 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride
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r
quinaldate + 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride + H2O
kynurenate + reduced 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride
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r
quinaldate + 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride + H2O
kynurenate + reduced 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride
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r
quinaldate + 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride + H2O
kynurenate + reduced 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride
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r
Quinaldate + acceptor
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inducible enzyme
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Quinaldate + acceptor
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inducible enzyme
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quinaldate + acceptor
kynurenate + reduced acceptor
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acceptors: 1,4-benzoquinone, 1,2-naphthoquinone, nitro blue tetrazolium chloride, 2,6-dichlorophenol indophenol, thionine, menadione, 2,4,6-trinitrobenzo sulfonic acid
i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate
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quinaldate + acceptor
kynurenate + reduced acceptor
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i.e. quinoline 2-carboxylic acid
i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate
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quinaldate + acceptor
kynurenate + reduced acceptor
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acceptors: phenazine methosulfate, medola's blue, cytochrome c, 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride
i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate
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quinaldate + acceptor
kynurenate + reduced acceptor
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acceptors: 1,4-benzoquinone, 1,2-naphthoquinone, nitro blue tetrazolium chloride, 2,6-dichlorophenol indophenol, thionine, menadione, 2,4,6-trinitrobenzo sulfonic acid
i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate
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quinaldate + acceptor
kynurenate + reduced acceptor
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i.e. quinoline 2-carboxylic acid
i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate
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quinaldate + acceptor
kynurenate + reduced acceptor
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acceptors: phenazine methosulfate, medola's blue, cytochrome c, 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride
i.e. 4-hydroxyquinoline 2-carboxylic acid. 20% of the activity with quinaldate
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quinaldate + acceptor
kynurenate + reduced acceptor
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acceptor: K3[Fe(CN)6]
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quinaldate + acceptor
kynurenate + reduced acceptor
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acceptors: phenazine methosulfate, medola's blue, cytochrome c, 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride
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quinaldate + acceptor
kynurenate + reduced acceptor
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acceptor: K3[Fe(CN)6]
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quinaldate + acceptor
kynurenate + reduced acceptor
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acceptors: phenazine methosulfate, medola's blue, cytochrome c, 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride
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quinoline 8-carboxylic acid + reduced acceptor
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quinoline 8-carboxylic acid + reduced acceptor
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Quinaldate + acceptor
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inducible enzyme
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Quinaldate + acceptor
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inducible enzyme
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FAD
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enzyme contains 1.8 mol of FAD per mol of enzyme
Pterin molybdenum cofactor
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molybdenum is probably associated with molybdopterin cytosine dinucleotide as organic part of the cofactor, 2 mol of molybdopterin cytosine dinucleotide per mol of enzyme
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Iron
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contains 7.6 g-atom of iron per mol of enzyme and 7.5 g-atom of acid-labile sulfur per mol of enzyme
Iron
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enzyme contains 2.3-3.7 gatom of iron per mol of enzyme
Molybdenum
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enzyme contains 1.6 g-atom per mol of enzyme
Molybdenum
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enzyme contains 0.5-0.6 gatom of molybdenum per mol of enzyme
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Cu2+
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KCN
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74% reactivation with S2-
methanol
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Mn2+
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NaAsO2
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p-hydroxymercuribenzoate
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p-hydroxymercuribenzoate
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0.085 - 1.6
2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride
0.085
2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride
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with quinaldate as cosubstrate
1.6
2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride
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with quinaldate as cosubstrate
0.023
Quinaldate
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with 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride as cosubstrate
1.45
Quinaldate
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with 2-(4-iodophenyl)-3-nitrophenyl-5-phenyltetrazolium chloride as cosubstrate
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AK-2
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brenda
AK-2
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brenda
2CC-1
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brenda
2CC-1
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brenda
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20000
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2 * 20000 + 2 * 32000 + 2 * 90000, SDS-PAGE
32000
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2 * 20000 + 2 * 32000 + 2 * 90000, SDS-PAGE
90000
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2 * 20000 + 2 * 32000 + 2 * 90000, SDS-PAGE
90000 - 100000
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gel filtration
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dimer
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1 * 18000-19000, beta, + 1 * 75000-80000, alpha, SDS-PAGE
dimer
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1 * 18000-19000, beta, + 1 * 75000-80000, alpha, SDS-PAGE
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hexamer
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2 * 20000 + 2 * 32000 + 2 * 90000, SDS-PAGE
hexamer
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2 * 20000 + 2 * 32000 + 2 * 90000, SDS-PAGE
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75
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5 min, 32% loss of activity
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ethylene glycol, 0.1%, Triton X-10, 0.1%, and Tween, 0.01%, inactivate the enzyme within 1-2 days at 4 C
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-80°C, 8 days, 25% loss of activity
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4°C, 8 days, 30% loss of activity
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Sauter, M.; Tshisuaka, B.; Fetzner, S.; Lingens, F.
XX. Quinaldic acid 4-oxidoreductase from Pseudomonas sp. AK-2 compared to other procaryotic molybdenum-containing hydroxylases
Biol. Chem. Hoppe-Seyler
374
1037-1046
1993
Pseudomonas sp., Pseudomonas sp. AK-2
brenda
Fetzner, S.; Lingens, F.
XVIII. Purification and some properties of the molybdenum- and iron-containing quinaldic acid 4-oxidoreductase from Serratia marcescens 2CC-1
Biol. Chem. Hoppe-Seyler
374
363-376
1993
Serratia marcescens, Serratia marcescens 2CC-1
brenda
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1.3.99.18 quinaldate 4-oxidoreductase
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