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EC Tree
The enzyme appears in viruses and cellular organisms
Synonyms
cis-1,2-dihydroxycyclohexa-3,5-diene-1-carboxylate dehydrogenase, dhb dehydrogenase, dihydrodihydroxybenzoate dehydrogenase, 3,5-cyclohexadiene-1,2-diol-1-carboxylic acid dehydrogenase,
more
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1,2-dihydroxycyclohexa-3,5-diene dehydrogenase
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1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate:NAD+ oxidoreductase (decarboxylating)
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2-hydro-1,2-dihydroxybenzoate dehydrogenase
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3,5-cyclohexadiene-1,2-diol-1-carboxylate dehydrogenase
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3,5-cyclohexadiene-1,2-diol-1-carboxylic acid dehydrogenase
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cis-1,2-dihydroxy-3,4-cyclohexadiene-1-carboxylate dehydrogenase
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cis-Diol dehydrogenase (Acinetobacter calcoaceticus reduced)
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cis-diol dehydrogenase (Pseudomonas putida reduced)
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dehydrogenase 1,2-dihydro-1,2-dihydroxybenzoate (Acinetobacter strain ADP1 gene benD)
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dehydrogenase, 1,2-dihydro-1,2-dihydroxybenzoate (Acinetobacter calcoaceticus strain ADP1 gene benD reduced)
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dehydrogenase, 1,2-dihydro-1,2-dihydroxybenzoate (plasmid pWWO clone pPL392 gene xylL reduced)
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dehydrogenase, 3,5-cyclohexadiene-1,2-diol-1-carboxylate
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dihydrodihydroxybenzoate dehydrogenase
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EC 1.3.1.55
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formerly
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GenBank AF009224-derived protein GI 2996624
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DHB dehydrogenase
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(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+ = catechol + CO2 + NADH + H+
(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+ = catechol + CO2 + NADH + H+
mechanism
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(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+ = catechol + CO2 + NADH + H+
mechanism
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(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+ = catechol + CO2 + NADH + H+
by growth on 3-chloro-, 4-chloro-, and 3,5-dichlorobenzoate enzyme is induced when combinated with recombinant toluate-1,2-dioxygenase
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(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+ = catechol + CO2 + NADH + H+
by growth on 3-chloro-, 4-chloro-, and 3,5-dichlorobenzoate enzyme is induced when combinated with recombinant toluate-1,2-dioxygenase
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(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+ = catechol + CO2 + NADH + H+
mechanism
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(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD+ = catechol + CO2 + NADH + H+
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(1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate:NAD+ oxidoreductase (decarboxylating)
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146888-19-9
formerly EC 1.3.1.55
146888-20-2
formerly EC 1.3.1.55
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(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+
catechol + CO2 + NADH
3-methyl-4,5-cyclohexadiene-cis-1,2-diol-1-carboxylic acid + NAD+
3-methylcatechol + CO2 + NADH
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3-methyldihydrodihydroxybenzoate + NAD+
3-methylcatechol + CO2 + NADH
cis-1,2-cyclohexanediol + NAD+
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at 95% of the activity with cis-benzene dihydrodiol
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cis-benzene dihydrodiol + NAD+
dihydroxybenzene + NADH
additional information
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(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+
catechol + CO2 + NADH
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(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+
catechol + CO2 + NADH
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(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+
catechol + CO2 + NADH
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enzyme in the pathway of dissimilation of benzoate via catechol
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(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+
catechol + CO2 + NADH
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(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+
catechol + CO2 + NADH
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free carboxyl groups essential
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(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+
catechol + CO2 + NADH
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(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+
catechol + CO2 + NADH
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(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+
catechol + CO2 + NADH
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(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+
catechol + CO2 + NADH
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(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+
catechol + CO2 + NADH
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enzyme is involved in the metabolism of benzene. A bedD mutant in which the gene is disrupted by a kanamycin resistance cassette is unable to utilize benzene for growth
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(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+
catechol + CO2 + NADH
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(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+
catechol + CO2 + NADH
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(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+
catechol + CO2 + NADH
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free carboxyl groups essential
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(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+
catechol + CO2 + NADH
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(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+
catechol + CO2 + NADH
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free carboxyl groups essential
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1,2-propanediol + NAD+
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at 189% of the activity with cis-benzene dihydrodiol
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1,2-propanediol + NAD+
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at 189% of the activity with cis-benzene dihydrodiol
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3-methyldihydrodihydroxybenzoate + NAD+
3-methylcatechol + CO2 + NADH
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assay substrate
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3-methyldihydrodihydroxybenzoate + NAD+
3-methylcatechol + CO2 + NADH
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assay substrate
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cis-benzene dihydrodiol + NAD+
dihydroxybenzene + NADH
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cis-benzene dihydrodiol + NAD+
dihydroxybenzene + NADH
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cis-benzene dihydrodiol + NAD+
dihydroxybenzene + NADH
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glycerol + NAD+
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at 18% of the activity with cis-benzene dihydrodiol
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glycerol + NAD+
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at 18% of the activity with cis-benzene dihydrodiol
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additional information
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no substrates: benzoic acid, cis-3,5-cyclohexadien-1,2-diol
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additional information
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purified recombinant enzyme also shows methyl transfer activity, methyl group is transferred from 4-methyl-3,5-cyclohexadiene-cis-1,2-diol-1-carboxylic acid to 3-methyl, only in presence of adenosylcobalamin as methyl group acceptor
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(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+
catechol + CO2 + NADH
(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+
catechol + CO2 + NADH
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enzyme in the pathway of dissimilation of benzoate via catechol
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(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+
catechol + CO2 + NADH
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(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+
catechol + CO2 + NADH
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free carboxyl groups essential
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(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+
catechol + CO2 + NADH
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(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+
catechol + CO2 + NADH
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(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+
catechol + CO2 + NADH
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(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+
catechol + CO2 + NADH
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(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+
catechol + CO2 + NADH
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enzyme is involved in the metabolism of benzene. A bedD mutant in which the gene is disrupted by a kanamycin resistance cassette is unable to utilize benzene for growth
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(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+
catechol + CO2 + NADH
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(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+
catechol + CO2 + NADH
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(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+
catechol + CO2 + NADH
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free carboxyl groups essential
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(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+
catechol + CO2 + NADH
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(-)-3,5-cyclohexadiene-1,2-diol-1-carboxylic acid + NAD+
catechol + CO2 + NADH
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free carboxyl groups essential
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?
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adenosylcobalamin
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required as methyl acceptor for the methyl transfer activity of recombinant enzyme from E. coli
NAD+
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m-toluate
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recombinant in Rhodobacter sphaeroides
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0.2
(-)-3,5-cyclohexadien-1,2-diol-1-carboxylate
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0.286
cis-benzene dihydrodiol
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additional information
additional information
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0.0435
NAD+
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additional information
additional information
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dependency on pH
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additional information
additional information
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substituted substrates
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additional information
additional information
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substituted substrates
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additional information
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wild-type enzyme, native and recombinant, and mutants, activity depending on growth substrates
additional information
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additional information
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8
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basonym Alcaligenes eutrophus
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brenda
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brenda
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brenda
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brenda
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brenda
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brenda
wild-type ADP1 and spontaneous mutants ADP136, ADP137, ADP138
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brenda
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brenda
only TOL plasmid is mentioned, which is derived from Pseudomonas putida, but not Pseudomonas putida itself
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brenda
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24000
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? * 24000, SDS-PAGE
28000
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? * 28000, DNA sequence determination, recombinant from Escherichia coli
31000
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2 * 31000, SDS-PAGE
94600
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sedimentation equilibrium centrifugation
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dimer
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2 * 31000, SDS-PAGE
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? * 24000, SDS-PAGE
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? * 28000, DNA sequence determination, recombinant from Escherichia coli
tetramer
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4 * 110000
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additional information
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operon insertion mutants of recombinant Escherichia coli expressing the TOL plasmid encoded enzymes including 1,2-dihydroxy--3,5-cyclohexadiene-1-carboxylate dehydrogenase, sequence analysis
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native enzyme: 10 min, 90% activity, recombinant enzyme: 5 min, complete inactivation
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partial, recombinant from Escherichia coli and Acinetobacter calcoaceticus
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recombinant from Escherichia coli
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addition of recombinant enzyme on plasmid TOL plasmid of donor PaW1 to native one in B13 derivative WR1
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expression of wild-type benD gene and native mutant genes in Escherichia coli and Acinetobacter calcoaceticus
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expression of xylL gene in Escherichia coli strain from TOL plasmid
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expression of xylL gene in Escherichia coli, plasmid hybrid pBR322 and pPl401
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expression of xylL gene in Rhodobacter sphaeroides encoded on TOL plasmid
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Neidle, E.; Hartnett, C.; Ornston, L.N.; Bairoch, A.; Rekik, M.; Harayama, S.
cis-Diol dehydrogenases encoded by the TOL pWW0 plasmid xylL gene and the Acinetobacter calcoaceticus chromosomal benD gene are members of the short-chain alcohol dehydrogenase superfamily
Eur. J. Biochem.
204
113-120
1992
Acinetobacter calcoaceticus
brenda
Fong, K.P.Y.; Goh, C.B.H.; Tan, H.M.
Characterization and expression of the plasmid-borne bedD gene from Pseudomonas putida ML2, which codes for a NAD+-dependent cis-benzene dihydrodiol dehydrogenase
J. Bacteriol.
178
5592-5601
1996
Pseudomonas putida, Pseudomonas putida ML2
brenda
Lee, J.Y.; Park, H.S.; Kim, H.S.
Adenosylcobalamin-mediated methyl transfer by toluate cis-dihydrodiol dehydrogenase of the TOL plasmid pWW0
J. Bacteriol.
181
2953-2957
1999
Pseudomonas sp., Pseudomonas putida
brenda
Neidle, E.L.; Shapiro, M.K.; Ornston, L.N.
Cloning and expression in Escherichia coli of Acinetobacter calcoaceticus genes for benzoate degradation
J. Bacteriol.
169
5496-5503
1987
Acinetobacter calcoaceticus
brenda
Lehrbach, P.R.; Zeyer, J.; Reineke, W.; Knackmuss, H.J.; Timmis, K.N.
Enzyme recruitment in vitro: use of cloned genes to extend the range of haloaromatics degraded by Pseudomonas sp. strain B13
J. Bacteriol.
158
1025-1032
1984
Pseudomonas putida
brenda
Reiner, A.M.
Metabolism of aromatic compounds in bacteria. Purification and properties of the catechol-forming enzyme, 3,5-cyclohexadiene-1,2-diol-1-carboxylic acid (NAD +) oxidoreductase (decarboxylating)
J. Biol. Chem.
247
4960-4965
1972
Cupriavidus necator
brenda
Harayama, S.; Rekik, M.
The meta cleavage operon of TOL degradative plasmid pWW0 comprises 13 genes
Mol. Gen. Genet.
221
113-120
1990
Pseudomonas putida
brenda
Reineke, W.; Knackmuss, H.J.
Hybrid pathway for chlorobenzoate metabolism in Pseudomonas sp. B13 derivatives
J. Bacteriol.
142
467-473
1980
Pseudomonas sp., Pseudomonas sp. B13
brenda
Zeyer, J.; Lehrbach, P.R.; Timmis, K.N.
Use of cloned genes of Pseudomonas TOL plasmid to effect biotransformation of benzoates to cis-dihydrodiols and catechols by Escherichia coli cells
Appl. Environ. Microbiol.
50
1409-1413
1985
Pseudomonas putida
brenda
Calero, S.; Gari, E.; Gibert, I.; Barbe, J.
Expression of the meta-cleavage pathway operon of the TOL plasmid of Pseudomonas putida in the phototrophic bacterium Rhodobacter sphaeroides
J. Biotechnol.
12
231-246
1989
Pseudomonas putida
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brenda
Reineke, W.; Knackmuss, H.J.
Chemical structure and biodegradability of halogenated aromatic compounds. Substituent effects on dehydrogenation of 3,5-cyclohexadiene-1,2-diol-1-carboxylic acid
Biochim. Biophys. Acta
542
424-429
1978
Cupriavidus necator, Pseudomonas sp., Pseudomonas sp. B13
brenda
Mason, J.R.; Geary, P.J.
cis-1,2-Dihydroxycyclohexa-3,5-diene (NAD) oxidoreductase cis-benzene dihydrodiol dehydrogenase from Pseudomonas putida NCIB 12190
Methods Enzymol.
23
134-137
1990
Pseudomonas putida, Pseudomonas putida NCIB12190
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brenda
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