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IUBMB Comments An iron-sulfur protein found in plant chloroplasts and cyanobacteria that contains a [4Fe-4S]cf . EC 1.17.7.3 ). The enzyme from the plant Arabidopsis thaliana is active with photoreduced 5-deazaflavin but not with flavodoxin .
Synonyms
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1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase
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4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase
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EC 1.17.4.3
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formerly
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gcpE gene product
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GcpE
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GcpE
Thermosynechococcus vestitus
ambiguous
IspG
ambiguous
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(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + oxidized ferredoxin = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + reduced ferredoxin + 2 H+
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MetaCyc
methylerythritol phosphate pathway II
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(E)-4-hydroxy-3-methylbut-2-en-1-yl-diphosphate:oxidized ferredoxin oxidoreductase
An iron-sulfur protein found in plant chloroplasts and cyanobacteria that contains a [4Fe-4S] cluster [1]. Forms part of an alternative non-mevalonate pathway for isoprenoid biosynthesis. Bacteria have a similar enzyme that uses flavodoxin rather than ferredoxin (cf. EC 1.17.7.3). The enzyme from the plant Arabidopsis thaliana is active with photoreduced 5-deazaflavin but not with flavodoxin [1].
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(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + oxidized ferredoxin
2-C-methyl-D-erythritol 2,4-cyclodiphosphate + reduced ferredoxin
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Substrates: -
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2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin
2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin
Substrates: -
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2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin
Substrates: the enzyme is involved in isoprenoid biosynthesis via the methylerythritol phosphate pathway
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2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin
Substrates: the enzyme is involved in the non-mevalonate pathway for isoprenoid biosynthesis
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2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin
Substrates: in the presence of light and via ferredoxin the electron flow from photosynthesis can directly provide the electrons required for the GcpE catalyzed reaction
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2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin
Thermosynechococcus vestitus
Substrates: -
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2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin
Thermosynechococcus vestitus
Substrates: essential enzyme of the non-mevalonate (2-C-methyl-D-erythritol-4-phosphate (MEP)) pathway for isoprenoid biosynthesis
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(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + oxidized ferredoxin
2-C-methyl-D-erythritol 2,4-cyclodiphosphate + reduced ferredoxin
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Substrates: -
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2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin
2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin
Substrates: the enzyme is involved in isoprenoid biosynthesis via the methylerythritol phosphate pathway
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2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin
Substrates: the enzyme is involved in the non-mevalonate pathway for isoprenoid biosynthesis
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2-C-methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H2O + 2 oxidized ferredoxin
Thermosynechococcus vestitus
Substrates: essential enzyme of the non-mevalonate (2-C-methyl-D-erythritol-4-phosphate (MEP)) pathway for isoprenoid biosynthesis
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[4Fe-4S]2+ cluster
Thermosynechococcus vestitus
[4Fe-4S]2 metalloprotein
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SwissProt
brenda
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brenda
Thermosynechococcus vestitus
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SwissProt
brenda
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brenda
Highest Expressing Human Cell Lines
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physiological function
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GcpE/IspG converts 2-C-methyl-D-erythritol-2,4-cyclodiphosphate into (E)-4-hydroxy-3-methyl-but-2-enyl diphosphate for isoprene biosynthesis
metabolism
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(E)-4-hydroxy-3-methylbut-2-enyl diphosphate synthase converts 2-C-methyl-D-erythritol-2,4-cyclodiphosphate into (E)-4-hydroxy-3-methyl-but-2-enyl diphosphate in the penultimate step of the methyl-erythritol phosphate pathway for isoprene biosynthesis, pathway overview
metabolism
Thermosynechococcus vestitus
essential enzyme of the non-mevalonate (2-C-methyl-D-erythritol-4-phosphate (MEP)) pathway for isoprenoid biosynthesis
metabolism
the enzyme is involved in isoprenoid biosynthesis via the methylerythritol phosphate pathway
metabolism
the enzyme is involved in the non-mevalonate pathway for isoprenoid biosynthesis
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x * 100000, SDS-PAGE
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x * 98700, calculated from amino acid sequence
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Ni-NTA agarose column chromatography, and Superdex 200 gel filtration
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recombinant GcpE protein is purified by an N-terminal His6 tag and reconstituted as a [4Fe-4S]2 metalloprotein
Thermosynechococcus vestitus
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expressed in Escherichia coli BL21(DE3) cells
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expression in Escherichia coli
Thermosynechococcus vestitus
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expression is induced by light
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Seemann, M.; Tse Sum Bui, B.; Wolff, M.; Miginiac-Maslow, M.; Rohmer, M.
Isoprenoid biosynthesis in plant chloroplasts via the MEP pathway: direct thylakoid/ferredoxin-dependent photoreduction of GcpE/IspG
FEBS Lett.
580
1547-1552
2006
Arabidopsis thaliana (F4K0E8), Arabidopsis thaliana
brenda
Okada, K.; Hase, T.
Cyanobacterial non-mevalonate pathway: (E)-4-hydroxy-3-methylbut-2-enyl diphosphate synthase interacts with ferredoxin in Thermosynechococcus elongatus BP-1
J. Biol. Chem.
280
20672-20679
2005
Thermosynechococcus vestitus (Q8DK70)
brenda
Hsieh, M.H.; Goodman, H.M.
The Arabidopsis IspH homolog is involved in the plastid nonmevalonate pathway of isoprenoid biosynthesis
Plant Physiol.
138
641-653
2005
Arabidopsis thaliana (F4K0E8)
brenda
Saggu, G.S.; Garg, S.; Pala, Z.R.; Yadav, S.K.; Kochar, S.K.; Kochar, D.K.; Saxena, V.
Characterization of 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (IspG) from Plasmodium vivax and its potential as an antimalarial drug target
Int. J. Biol. Macromol.
96
466-473
2017
Plasmodium vivax
brenda
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