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4-amino-L-phenylalanyl-[CmlP-peptidyl-carrier-protein],acceptor:oxygen 3-oxidoreductase
The enzyme, characterized from the bacterium Streptomyces venezuelae, participates in the biosynthesis of the antibiotic chloramphenicol. It carries an oxygen-bridged dinuclear iron cluster. The native electron donor remains unknown, and the enzyme was assayed in vitro using sodium dithionite. The enzyme only acts on its substrate when it is loaded onto the peptidyl-carrier domain of the CmlP non-ribosomal peptide synthase.
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4-amino-L-phenylalanyl-[CmlP-peptidyl-carrier-protein] + reduced acceptor + O2
2-(4-aminophenyl)-L-seryl-[CmlP-peptidyl-carrier-protein] + acceptor + H2O
additional information
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4-amino-L-phenylalanyl-[CmlP-peptidyl-carrier-protein] + reduced acceptor + O2
2-(4-aminophenyl)-L-seryl-[CmlP-peptidyl-carrier-protein] + acceptor + H2O
Substrates: -
Products: -
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4-amino-L-phenylalanyl-[CmlP-peptidyl-carrier-protein] + reduced acceptor + O2
2-(4-aminophenyl)-L-seryl-[CmlP-peptidyl-carrier-protein] + acceptor + H2O
Substrates: -
Products: -
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additional information
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Substrates: CmlA contains an oxo-bridged dinuclear iron cluster. CmlA is functional in the diferrous state, and substrate L-PAPA is covalently bound to CmlP. The product is hydroxylated L-PAPA and O2 is the oxygen source
Products: -
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additional information
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Substrates: CmlA contains an oxo-bridged dinuclear iron cluster. CmlA is functional in the diferrous state, and substrate L-PAPA is covalently bound to CmlP. The product is hydroxylated L-PAPA and O2 is the oxygen source
Products: -
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Makris, T.M.; Knoot, C.J.; Wilmot, C.M.; Lipscomb, J.D.
Structure of a dinuclear iron cluster-containing beta-hydroxylase active in antibiotic biosynthesis
Biochemistry
52
6662-6671
2013
Streptomyces venezuelae (F2RB80), Streptomyces venezuelae DSM 40230 (F2RB80)
brenda
Jasniewski, A.J.; Knoot, C.J.; Lipscomb, J.D.; Que, L.
A carboxylate shift regulates dioxygen activation by the diiron nonheme beta-hydroxylase CmlA upon binding of a substrate-loaded nonribosomal peptide synthetase
Biochemistry
55
5818-5831
2016
Streptomyces venezuelae (F2RB80), Streptomyces venezuelae DSM 40230 (F2RB80)
brenda
Tanaka, E.; Taniguchi, T.; Sawa, Y.; Ohmori, S.; Kitada, M.; Horie, T.
Trimethadione metabolism and microsomal monooxygenases in untreated and phenobarbital-treated rhesus monkeys
Comp. Biochem. Physiol. C
120
317-320
1998
Macaca mulatta
brenda
Vu, V.V.; Makris, T.M.; Lipscomb, J.D.; Que, L.
Active-site structure of a beta-hydroxylase in antibiotic biosynthesis
J. Am. Chem. Soc.
133
6938-6941
2011
Streptomyces venezuelae (F2RB80), Streptomyces venezuelae DSM 40230 (F2RB80)
brenda
Makris, T.M.; Chakrabarti, M.; Muenck, E.; Lipscomb, J.D.
A family of diiron monooxygenases catalyzing amino acid beta-hydroxylation in antibiotic biosynthesis
Proc. Natl. Acad. Sci. USA
107
15391-15396
2010
Streptomyces venezuelae (F2RB80), Streptomyces venezuelae DSM 40230 (F2RB80)
brenda
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