Any feedback?
No. of entries
Information on EC 1.14.99.38 - cholesterol 25-monooxygenase
for references in articles please use BRENDA:EC1.14.99.38
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree 1 Oxidoreductases
1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
1.14.99 Miscellaneous
1.14.99.38 cholesterol 25-monooxygenase
IUBMB Comments
Unlike most other sterol hydroxylases, this enzyme is not a cytochrome P-450. Instead, it uses diiron cofactors to catalyse the hydroxylation of hydrophobic substrates . The diiron cofactor can be either Fe-O-Fe or Fe-OH-Fe and is bound to the enzyme through interactions with clustered histidine or glutamate residues [4,5]. In cell cultures, this enzyme down-regulates cholesterol synthesis and the processing of sterol regulatory element binding proteins (SREBPs). cf. EC 1.17.99.10, cholesterol C-25 hydroxylase.
The enzyme appears in viruses and cellular organisms
- 1.14.99.38
-
pregnane
-
cyp2b10
- 25-hydroxycholesterol
-
androstane
- oxysterols
-
drug-metabolizing
- phenobarbital
-
cyp2c29
- ugt1a1
-
interferon-stimulated
-
cyp3a13
-
apap-induced
-
lithocholic
-
acetaminophen-induced
-
pxr-mediated
-
cyp2a4
-
pxr-null
-
tcpobop
- medicine
showSynonyms
cyp3a11, ch25h, cholesterol-25-hydroxylase, more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
CH25H
cholesterol 25-hydroxylase
cholesterol 25-monooxygenase
cholesterol-25-hydroxylase
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cholesterol + reduced acceptor + O2 = 25-hydroxycholesterol + acceptor + H2O
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
reduction
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PATHWAY SOURCE
PATHWAYS
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
cholesterol,hydrogen-donor:oxygen oxidoreductase (25-hydroxylating)
Unlike most other sterol hydroxylases, this enzyme is not a cytochrome P-450. Instead, it uses diiron cofactors to catalyse the hydroxylation of hydrophobic substrates [1]. The diiron cofactor can be either Fe-O-Fe or Fe-OH-Fe and is bound to the enzyme through interactions with clustered histidine or glutamate residues [4,5]. In cell cultures, this enzyme down-regulates cholesterol synthesis and the processing of sterol regulatory element binding proteins (SREBPs). cf. EC 1.17.99.10, cholesterol C-25 hydroxylase.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CAS REGISTRY NUMBER
COMMENTARY
60202-07-5
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
Substrates: -
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
-
Substrates: -
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
Substrates: -
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
-
Substrates: -
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
-
Substrates: -
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
Substrates: -
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
-
Substrates: -
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
Substrates: -
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
Substrates: -
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
Substrates: -
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
Substrates: cholesterol 25-hydroxylase has the capacity to play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein processing and ultimately leads to inhibition of gene transcription
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
-
Substrates: genetic variability in the set of cholesterol metabolism genes: cholesterol 25-hydroxylase, cholesterol 24-hydroxylase and ATP-binding cassette transporter A1 does not influence the development of AlzheimerĀs disease
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
Substrates: the enzyme has the capacity to synthesize an active and potent regulator of the sterol response element binding protein pathway within the cell
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
Substrates: lower 25-hydroxylation enzyme activity by CYP1A2 compared to marker enzyme CYP3A4
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
Substrates: lower 25-hydroxylation enzyme activity by CYP2C9 compared to marker enzyme CYP3A4
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
Substrates: lower 25-hydroxylation enzyme activity by CYP2D6 compared to marker enzyme CYP3A4
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
Substrates: production of 25-hydroxycholesterol by intact CYP3A4, most significant activity is 25-hydroxylation, which is higher than that of 4beta-hydroxylation, a marker activity of CYP3A4
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
Substrates: the reaction might be catalyzed by CYP3A4
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
Substrates: -
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
-
Substrates: -
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
Substrates: -
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
Substrates: -
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
Substrates: cholesterol 25-hydroxylase has the capacity to play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein processing and ultimately leads to inhibition of gene transcription
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
Substrates: the enzyme has the capacity to synthesize an active and potent regulator of the sterol response element binding protein pathway within the cell
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
Substrates: the reaction might be catalyzed by CYP3A4
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
-
Substrates: -
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
Substrates: -
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
-
Substrates: testicular macrophagers play an important role in the differentiation of Leydig cells through the secretion of 25-hydroxycholesterol
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
-
Substrates: expression of 25-hydroxylase mRNA and production of 25-hydroxycholesterol by cultured testicular macrophages is significantly inhibited by testosterone at 0.01 mg/ml
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
-
Substrates: -
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
-
Substrates: -
Products: -
Products: -
?
additional information
?
-
Substrates: 25-hydroxycholesterol is a secondary autoxidation product derived from 3beta-hydroxy-cholest-5-ene-25-hydroperoxide, a hydroperoxide identified in air-aged cholesterol. Thermal decomposition of 3beta-hydroxy-cholest-5-ene-25-hydroperoxide gives rise to 25-hydroxycholesterol
Products: -
Products: -
?
additional information
?
-
Substrates: 25-hydroxycholesterol is a secondary autoxidation product derived from 3beta-hydroxy-cholest-5-ene-25-hydroperoxide, a hydroperoxide identified in air-aged cholesterol. Thermal decomposition of 3beta-hydroxy-cholest-5-ene-25-hydroperoxide gives rise to 25-hydroxycholesterol
Products: -
Products: -
?
additional information
?
-
-
Substrates: 25-hydroxycholesterol is a secondary autoxidation product derived from 3beta-hydroxy-cholest-5-ene-25-hydroperoxide, a hydroperoxide identified in air-aged cholesterol. Thermal decomposition of 3beta-hydroxy-cholest-5-ene-25-hydroperoxide gives rise to 25-hydroxycholesterol
Products: -
Products: -
?
additional information
?
-
-
Substrates: 25-hydroxycholesterol is a secondary autoxidation product derived from 3beta-hydroxy-cholest-5-ene-25-hydroperoxide, a hydroperoxide identified in air-aged cholesterol. Thermal decomposition of 3beta-hydroxy-cholest-5-ene-25-hydroperoxide gives rise to 25-hydroxycholesterol
Products: -
Products: -
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
Substrates: -
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
-
Substrates: -
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
Substrates: -
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
-
Substrates: -
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
-
Substrates: -
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
Substrates: -
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
Substrates: -
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
Substrates: cholesterol 25-hydroxylase has the capacity to play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein processing and ultimately leads to inhibition of gene transcription
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
-
Substrates: genetic variability in the set of cholesterol metabolism genes: cholesterol 25-hydroxylase, cholesterol 24-hydroxylase and ATP-binding cassette transporter A1 does not influence the development of AlzheimerĀs disease
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
Substrates: the enzyme has the capacity to synthesize an active and potent regulator of the sterol response element binding protein pathway within the cell
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
Substrates: lower 25-hydroxylation enzyme activity by CYP1A2 compared to marker enzyme CYP3A4
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
Substrates: lower 25-hydroxylation enzyme activity by CYP2C9 compared to marker enzyme CYP3A4
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
Substrates: lower 25-hydroxylation enzyme activity by CYP2D6 compared to marker enzyme CYP3A4
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
Substrates: production of 25-hydroxycholesterol by intact CYP3A4, most significant activity is 25-hydroxylation, which is higher than that of 4beta-hydroxylation, a marker activity of CYP3A4
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
Substrates: the reaction might be catalyzed by CYP3A4
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
Substrates: -
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
-
Substrates: -
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
Substrates: -
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
Substrates: cholesterol 25-hydroxylase has the capacity to play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein processing and ultimately leads to inhibition of gene transcription
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
Substrates: the enzyme has the capacity to synthesize an active and potent regulator of the sterol response element binding protein pathway within the cell
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
Substrates: the reaction might be catalyzed by CYP3A4
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
-
Substrates: -
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
Substrates: -
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
-
Substrates: testicular macrophagers play an important role in the differentiation of Leydig cells through the secretion of 25-hydroxycholesterol
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
-
Substrates: -
Products: -
Products: -
?
cholesterol + AH2 + O2
25-hydroxycholesterol + A + H2O
-
Substrates: -
Products: -
Products: -
?
additional information
?
-
Substrates: 25-hydroxycholesterol is a secondary autoxidation product derived from 3beta-hydroxy-cholest-5-ene-25-hydroperoxide, a hydroperoxide identified in air-aged cholesterol. Thermal decomposition of 3beta-hydroxy-cholest-5-ene-25-hydroperoxide gives rise to 25-hydroxycholesterol
Products: -
Products: -
?
additional information
?
-
Substrates: 25-hydroxycholesterol is a secondary autoxidation product derived from 3beta-hydroxy-cholest-5-ene-25-hydroperoxide, a hydroperoxide identified in air-aged cholesterol. Thermal decomposition of 3beta-hydroxy-cholest-5-ene-25-hydroperoxide gives rise to 25-hydroxycholesterol
Products: -
Products: -
?
additional information
?
-
-
Substrates: 25-hydroxycholesterol is a secondary autoxidation product derived from 3beta-hydroxy-cholest-5-ene-25-hydroperoxide, a hydroperoxide identified in air-aged cholesterol. Thermal decomposition of 3beta-hydroxy-cholest-5-ene-25-hydroperoxide gives rise to 25-hydroxycholesterol
Products: -
Products: -
?
additional information
?
-
-
Substrates: 25-hydroxycholesterol is a secondary autoxidation product derived from 3beta-hydroxy-cholest-5-ene-25-hydroperoxide, a hydroperoxide identified in air-aged cholesterol. Thermal decomposition of 3beta-hydroxy-cholest-5-ene-25-hydroperoxide gives rise to 25-hydroxycholesterol
Products: -
Products: -
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Fe
Fe2+
Fe
diiron-containing enzyme. The iron is present as Fe-O-Fe or Fe-OH-Fe and is coordinated with clusters of conserved histidine residues
Fe
diiron-containing enzyme. The iron is present as Fe-O-Fe or Fe-OH-Fe and is coordinated with clusters of conserved histidine residues
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
desmosterol
a potent inhibitor of CH25H; a potent inhibitor of CH25H; a potent inhibitor of CH25H
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
incubation with LPS derived from Escherichia coli or Salmonella enterica induces CH25H mRNA
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
additional information
patients with chronic obstructive pulmonary disease have increased expression of cholesterol 25-hydroxylase in the lung
brenda
-
testicular, 10-day-old animals have the highest steady-state levels of message
brenda
additional information
25-hydroxycholesterol concentrations in normal human sera correlates positively with the levels of 4beta-hydroxycholesterol, but does not significantly correlate with the levels of 27-hydroxycholesterol or (24S)-hydroxycholesterol
brenda
additional information
25-hydroxycholesterol concentrations in normal human sera correlates positively with the levels of 4beta-hydroxycholesterol, but does not significantly correlate with the levels of 27-hydroxycholesterol or (24S)-hydroxycholesterol
brenda
additional information
25-hydroxycholesterol concentrations in normal human sera correlates positively with the levels of 4beta-hydroxycholesterol, but does not significantly correlate with the levels of 27-hydroxycholesterol or (24S)-hydroxycholesterol
brenda
additional information
25-hydroxycholesterol concentrations in normal human sera correlates positively with the levels of 4beta-hydroxycholesterol, but does not significantly correlate with the levels of 27-hydroxycholesterol or (24S)-hydroxycholesterol
brenda
additional information
-
quantitative enzyme expression analysis in macrophages and dendritic cells, overview
brenda
additional information
-
quantitative enzyme expression analysis in macrophages and dendritic cells, overview
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE