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24,25-dihydrolanosterol + 6 reduced ferredoxin [iron-sulfur] cluster + 6 H+ + 3 O2
4,4-dimethyl-5alpha-cholesta-8,14-diene-3beta-ol + formate + 6 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O
estriol + 6 reduced ferredoxin [iron-sulfur] cluster + 6 H+ + 3 O2
? + formate + 6 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O
hydroxylanosterol + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
oxolanosterol + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
lanosterol + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
hydroxylanosterol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
lanosterol + 6 reduced ferredoxin [iron-sulfur] cluster + 6 H+ + 3 O2
4,4-dimethylzymosterol + formate + 6 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O
lanosterol + 6 reduced ferredoxin [iron-sulfur] cluster + 6 H+ + 3 O2
? + formate + 6 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O
-
-
-
-
?
lanosterol + [reduced NADPH-hemoprotein reductase] + O2
? + formate + [oxidized NADPH-hemoprotein reductase] + H2O
-
activity depends on presence of natural fusion protein ferredoxin
-
-
?
obtusifoliol + 6 reduced ferredoxin [iron-sulfur] cluster + 6 H+ + 3 O2
4alpha-methyl-5alpha-ergost-8,14,24(28)-trien-3beta-ol + formate + 6 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O
oxolanosterol + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
4,4-dimethylzymosterol + formate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
additional information
?
-
24,25-dihydrolanosterol + 6 reduced ferredoxin [iron-sulfur] cluster + 6 H+ + 3 O2
4,4-dimethyl-5alpha-cholesta-8,14-diene-3beta-ol + formate + 6 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O
-
-
-
?
24,25-dihydrolanosterol + 6 reduced ferredoxin [iron-sulfur] cluster + 6 H+ + 3 O2
4,4-dimethyl-5alpha-cholesta-8,14-diene-3beta-ol + formate + 6 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O
-
-
-
?
estriol + 6 reduced ferredoxin [iron-sulfur] cluster + 6 H+ + 3 O2
? + formate + 6 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O
-
-
-
?
estriol + 6 reduced ferredoxin [iron-sulfur] cluster + 6 H+ + 3 O2
? + formate + 6 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O
-
-
-
?
hydroxylanosterol + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
oxolanosterol + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
M2UZT3
-
-
-
?
hydroxylanosterol + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
oxolanosterol + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
M2UZT3
-
-
-
?
lanosterol + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
hydroxylanosterol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
M2UZT3
-
-
-
?
lanosterol + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
hydroxylanosterol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
M2UZT3
-
-
-
?
lanosterol + 6 reduced ferredoxin [iron-sulfur] cluster + 6 H+ + 3 O2
4,4-dimethylzymosterol + formate + 6 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O
-
-
-
?
lanosterol + 6 reduced ferredoxin [iron-sulfur] cluster + 6 H+ + 3 O2
4,4-dimethylzymosterol + formate + 6 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O
-
-
-
?
lanosterol + 6 reduced ferredoxin [iron-sulfur] cluster + 6 H+ + 3 O2
4,4-dimethylzymosterol + formate + 6 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O
-
-
-
?
lanosterol + 6 reduced ferredoxin [iron-sulfur] cluster + 6 H+ + 3 O2
4,4-dimethylzymosterol + formate + 6 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O
-
-
-
?
lanosterol + 6 reduced ferredoxin [iron-sulfur] cluster + 6 H+ + 3 O2
4,4-dimethylzymosterol + formate + 6 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O
M2UZT3
-
-
-
?
lanosterol + 6 reduced ferredoxin [iron-sulfur] cluster + 6 H+ + 3 O2
4,4-dimethylzymosterol + formate + 6 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O
M2UZT3
-
-
-
?
obtusifoliol + 6 reduced ferredoxin [iron-sulfur] cluster + 6 H+ + 3 O2
4alpha-methyl-5alpha-ergost-8,14,24(28)-trien-3beta-ol + formate + 6 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O
-
-
-
?
obtusifoliol + 6 reduced ferredoxin [iron-sulfur] cluster + 6 H+ + 3 O2
4alpha-methyl-5alpha-ergost-8,14,24(28)-trien-3beta-ol + formate + 6 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O
-
-
-
?
oxolanosterol + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
4,4-dimethylzymosterol + formate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
M2UZT3
-
-
-
?
oxolanosterol + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
4,4-dimethylzymosterol + formate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
M2UZT3
-
-
-
?
additional information
?
-
both flavodoxin and ferredoxin redox systems are able to support the enzymatic activity. Substrates require a 14alpha methyl group and a 8,9 C-C double bond
-
-
?
additional information
?
-
both flavodoxin and ferredoxin redox systems are able to support the enzymatic activity. Substrates require a 14alpha methyl group and a 8,9 C-C double bond
-
-
?
additional information
?
-
M2UZT3
exogenous electron donor systems, Fdx-FdR i.e. Acinetobacter sp. OC4 ferredoxin and ferredoxin reductase, Fld-FdR2 i.e. Escherichia coli flavodoxin and flavodoxin reductase, and Nocardia farcinica iron-sulfur containing NADPH-P450 reductase NfFdR can be used. Fdx-FdR is the most efficient electron donor
-
-
?
additional information
?
-
M2UZT3
exogenous electron donor systems, Fdx-FdR i.e. Acinetobacter sp. OC4 ferredoxin and ferredoxin reductase, Fld-FdR2 i.e. Escherichia coli flavodoxin and flavodoxin reductase, and Nocardia farcinica iron-sulfur containing NADPH-P450 reductase NfFdR can be used. Fdx-FdR is the most efficient electron donor
-
-
?
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Jackson, C.J.; Lamb, D.C.; Marczylo, T.H.; Warrilow, A.G.; Manning, N.J.; Lowe, D.J.; Kelly, D.E.; Kelly, S.L.
A novel sterol 14alpha-demethylase/ferredoxin fusion protein (MCCYP51FX) from Methylococcus capsulatus represents a new class of the cytochrome P450 superfamily
J. Biol. Chem.
277
46959-46965
2002
Methylococcus capsulatus
brenda
Ke, X.; Ding, G.; Ma, B.; Liu, Z.; Zhang, J.; Zheng, Y.
Characterization of a novel CYP51 from Rhodococcus triatomae and its NADH-ferredoxin reductase-coupled application in lanosterol 14alpha-demethylation
Process Biochem.
62
59-68
2017
Rhodococcus triatomae (M2UZT3), Rhodococcus triatomae BKS 15-14 (M2UZT3)
-
brenda
Jackson, C.J.; Lamb, D.C.; Marczylo, T.H.; Parker, J.E.; Manning, N.L.; Kelly, D.E.; Kelly, S.L.
Conservation and cloning of CYP51 a sterol 14 alpha-demethylase from Mycobacterium smegmatis
Biochem. Biophys. Res. Commun.
301
558-563
2003
Mycolicibacterium smegmatis
brenda
McLean, K.J.; Warman, A.J.; Seward, H.E.; Marshall, K.R.; Girvan, H.M.; Cheesman, M.R.; Waterman, M.R.; Munro, A.W.
Biophysical characterization of the sterol demethylase P450 from Mycobacterium tuberculosis, its cognate ferredoxin, and their interactions
Biochemistry
45
8427-8443
2006
Mycobacterium tuberculosis (P9WPP9), Mycobacterium tuberculosis H37Rv (P9WPP9)
brenda
Choi, K.Y.; Jung, E.O.; Jung, D.H.; Pandey, B.P.; Lee, N.; Yun, H.; Park, H.Y.; Kim, B.G.
Novel iron-sulfur containing NADPH-reductase from Nocardia farcinica IFM10152 and fusion construction with CYP51 lanosterol demethylase
Biotechnol. Bioeng.
109
630-636
2012
Nocardia farcinica (Q5YWK5), Nocardia farcinica IFM 10152 (Q5YWK5)
brenda
Bellamine, A.; Mangla, A.T.; Nes, W.D.; Waterman, M.R.
Characterization and catalytic properties of the sterol 14alpha-demethylase from Mycobacterium tuberculosis
Proc. Natl. Acad. Sci. USA
96
8937-8942
1999
Mycobacterium tuberculosis (P9WPP9), Mycobacterium tuberculosis H37Rv (P9WPP9)
brenda